Microfluidic devices, and methods of making and using the same
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/558
B01L-003/00
B01F-013/00
B01F-005/06
출원번호
US-0703928
(2017-09-13)
등록번호
US-10073093
(2018-09-11)
발명자
/ 주소
Bornheimer, Scott Joseph
Sugarman, Jeffrey
Huang, Wei
Goldberg, Edward Michael
Tan, Ming
출원인 / 주소
Becton, Dickinson and Company
대리인 / 주소
Field, Bret E.
인용정보
피인용 횟수 :
0인용 특허 :
127
초록▼
The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a
The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.
대표청구항▼
1. An assay method using a microfluidic device; the method comprising: (a) providing a microfluidic device comprising: a sample application site;a flow channel in fluid communication with the sample application site; anda porous component positioned between the sample application site and flow chann
1. An assay method using a microfluidic device; the method comprising: (a) providing a microfluidic device comprising: a sample application site;a flow channel in fluid communication with the sample application site; anda porous component positioned between the sample application site and flow channel, wherein the porous component comprises a porous matrix comprising pores and an assay reagent comprising an analyte-specific optically detectable label positioned within the pores of the porous matrix;(b) contacting a sample to the sample application site so that the sample flows through the porous matrix and into the flow channel, wherein the porous matrix is configured to mix the sample with the assay reagent to produce a labelled sample that then flows into the flow channel;(c) illuminating the labelled sample in the flow channel with a light source; and(d) detecting light from the labelled sample. 2. The method according to claim 1, wherein the analyte-specific optically detectable label comprises an analyte-specific binding member conjugated to an optically detectable label. 3. The method according to claim 2, wherein the analyte-specific binding member is an antibody or antibody fragment. 4. The method according to claim 3, wherein the antibody or antibody fragment specifically binds to a target selected from the group consisting of CD14, CD4, CD45RA, CD3 and a combination thereof. 5. The method according to claim 2, wherein the optically detectable label comprises a fluorescent dye. 6. The method according to claim 5, wherein the fluorescent dye comprises a compound selected from the group consisting of rhodamine, coumarin, cyanine, xanthene, polymethine, pyrene, dipyrromethene borondifluoride, napthalimide, phycobiliprotein, peridinium chlorophyll proteins, conjugates thereof and a combination thereof. 7. The method according to claim 1, wherein 95% or greater of the sample passes through the porous matrix into the flow channel. 8. The method according to claim 1, wherein the method comprises illuminating the sample with a broad spectrum light source. 9. The method according to claim 8, wherein the broad spectrum light source comprises an ultraviolet light source and a visible light source. 10. The method according to claim 8, wherein the method comprises illuminating the sample with light having a wavelength between 200 nm and 800 nm. 11. The method according to claim 1, wherein detecting light from the labelled sample comprises capturing an image of the sample in the flow channel. 12. The method according to claim 1, wherein the sample is a biological fluid. 13. The method according to claim 12, wherein the biological fluid is whole blood. 14. The method according to claim 12, wherein the biological fluid is plasma. 15. The method according to claim 1, wherein the assay reagent is positioned within the pores of the porous matrix. 16. The method according to claim 15, wherein the assay reagent is dry. 17. The method according to claim 1, wherein the porous matrix comprises pores having diameters between 1 μm and 200 μm. 18. An assay method using a microfluidic device; the method comprising: contacting a sample to a sample application site of a microfluidic device, the microfluidic device comprising: a flow channel in fluid communication with the sample application site; anda porous component positioned between the sample application site and flow channel, wherein the porous component comprises a porous matrix and an assay reagent;wherein the porous matrix comprises pores; wherein the assay reagent is positioned within the pores of the porous reagent; andwherein the porous matrix is configured to mix the sample with the assay reagent to produce a labeled sample that flows into the flow channel. 19. The method according to claim 18, wherein the assay reagent comprises an analyte-specific binding member conjugated to an optically detectable label. 20. The method according to claim 19, wherein the analyte-specific binding member is an antibody or antibody fragment.
Brooker Gary (Potomac MD) McDonald J. Scott (Germantown MD) Brooker Jeffrey S. (Herndon VA), Apparatus and method for multiple emission ratio photometry and multiple emission ratio imaging.
Baer Thomas M. (Mountain View CA) Dietz Louis J. (Mountain View CA) Dubrow Robert S. (San Carlos CA) Hayter Paul G. (Los Altos CA) Hodges Michael (Palo Alto CA) Manian Bala S. (Los Altos Hills CA) Sh, Apparatus and method for volumetric capillary cytometry.
Aslund Nils R. D. (Skontorpsvgen 126 ; 9 tr S-121 65 Johanneshov SEX) Carlsson Kjell S. (Malmbodavgen 17 S-186 42 Vallentuna SEX), Apparatus for quantitative imaging of multiple fluorophores.
Watanabe Sadakazu (Kawasaki JA) Shinoda Hidenori (Yokohama JA), Apparatus for selectively segmenting red and white blood corpuscles contained in blood smear.
Andrews Jeffrey P. ; O'Keefe Christian V. ; Scrivens Brian G. ; Pope Willard C. ; Hansen Timothy ; Failing Frank L., Automated optical reader for nucleic acid assays.
Berndt Klaus W. (Baltimore) Gryczynski Ignacy (Baltimore) Lakowicz Joseph R. (Columbia MD), Fluorometry method and apparatus using a semiconductor laser diode as a light source.
Brooker Gary (9212 Bentridge Ave. Potomac MD 20854), High-speed multiple wavelength illumination source, apparatus containing the same, and applications thereof to methods o.
Zeng,Haishan; Lam,Stephen; Palcic,Branko Mihael, Imaging methods for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices.
Stimpson Donald I. (Gurnee IL) Gordon Julian (Lake Bluff IL) Hoijer Joanell V. (Arlington Heights IL), Light scattering optical waveguide method for detecting specific binding events.
Peters Richard K. (Tallmadge OH) Elmerick Donald V. (Tallmadge OH) Spayer James L. (Brecksville OH) Walter Gerald E. (Cleveland OH 4), Method and apparatus for cell analysis.
James K. Riley ; Michael G. Meyer ; David J. Perry ; Andrew D. Silber, Method and apparatus for deriving separate images from multiple chromogens in a branched image analysis system.
Wardlaw, Stephen C.; Levine, Robert A.; Unfricht, Darryn W.; Lalpuria, Niten V.; Hill, Jeremy R., Method and apparatus for determining red blood cell indices of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells.
Brunhouse Robert F. (Coral Gables FL) Hajek Constance M. (Miami Lakes FL) Russell Thomas (Miami FL) Coulter Wallace H. (Miami Springs FL), Method and apparatus for obtaining an absolute white blood cell subset count and white blood cell multipart differential.
Hudson James Carey ; Russell Thomas ; Rodriguez Carlos M. ; Coulter Wallace H., Method and apparatus for screening obscured or partially obscured cells.
Sterling, Bernhard B.; Braig, James R.; Goldberger, Daniel S.; Hartstein, Philip C.; Gaffney, Robert D., Method of determining an analyte concentration in a sample from an absorption spectrum.
Sterling,Bernhard B.; Braig,James R.; Goldberger,Daniel S.; Witte,Kenneth G., Method of determining an analyte concentration in a sample from an absorption spectrum.
Sterling,Bernhard B.; Braig,James R.; Goldberger,Daniel S.; Hartstein,Philip C.; Gaffney,Robert D., Method of determining analyte concentration in a sample using infrared transmission data.
Zeng, Haishan; Lam, Stephen; Palcic, Branko Michael, Methods and apparatus for fluorescence and reflectance imaging and spectroscopy and for contemporaneous measurements of electromagnetic radiation with multiple measuring devices.
McDevitt, John T.; Christodoulides, Nicolaos J.; Floriano, Pierre N.; Douglas, Gary N.; Rogers, Patrick E., Methods and compositions related to determination and use of white blood cell counts.
Modlin, Douglas N.; Owicki, John C.; Petersen, Jon F.; French, Todd E.; Wright, Carl L.; Ruiz, Jeanne A.; Bechtel, Lorne E., Multi-mode light detection system.
Rice Mark J. ; Sweat ; Jr. Robert H. ; Rioux James M. ; Williams William T. ; Routt Wilson, Non-invasive measurement of blood component using retinal imaging.
Mark J. Rice ; Robert H. Sweat, Jr. ; James M. Rioux ; William T. Williams ; Wilson Routt, Non-invasive measurement of blood components using retinal imaging.
Ward Anthony J. (San Ramon CA) Mercolino Thomas J. (Pleasanton CA) Recktenwald Diether J. (Cupertino CA), One step method for detection and enumeration of absolute counts of one more cell populations in a sample.
Lee Wylie I. ; Alderete Jason E. ; Fowler William V.CTY Minneapolis, Optical measurement of blood hematocrit incorporating a self-calibration algorithm.
Lilge Lothar,CAX ; Pennefather Peter S.,CAX ; Ross Stephen M.,CAX ; Tang Cha-Min ; Zhang Kai,CAX, Semiconductor based excitation illuminator for fluorescence and phosphorescence microscopy.
Lindberg John M. ; McGlashen Michael L., Sensor utilizing Raman spectroscopy for non-invasive monitoring of analytes in biological fluid and method of use.
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