Method of preparing an adipose tissue derived matrix
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-035/35
A61L-027/36
출원번호
US-0388428
(2016-12-22)
등록번호
US-10092600
(2018-10-09)
발명자
/ 주소
Huang, Yen-Chen
Ivery, Asia
Choi, Bryan
Schilling, Benjamin
Ngo, Manh-Dan
출원인 / 주소
Musculoskeletal Transplant Foundation
대리인 / 주소
Cole Schotz, P.C.
인용정보
피인용 횟수 :
0인용 특허 :
250
초록▼
An acellular soft tissue-derived matrix includes a collagenous tissue that has been delipidated and decellularized. Adipose tissue is among the soft tissues suitable for manufacturing an acellular soft tissue-derived matrix. Exogenous tissuegenic cells and other biologically-active factors may be ad
An acellular soft tissue-derived matrix includes a collagenous tissue that has been delipidated and decellularized. Adipose tissue is among the soft tissues suitable for manufacturing an acellular soft tissue-derived matrix. Exogenous tissuegenic cells and other biologically-active factors may be added to the acellular matrix. The acellular matrix may be provided as particles, a slurry, a paste, a gel, or in some other form. The acellular matrix may be provided as a three-dimensional scaffold that has been reconstituted from particles of the three-dimensional tissue. The three-dimensional scaffold may have the shape of an anatomical feature and serve as a template for tissue repair or replacement. A method of making an acellular soft tissue-derived matrix includes steps of removing lipid from the soft tissue by solvent extraction and chemical decellularization of the soft tissue.
대표청구항▼
1. A method of preparing an adipose tissue-derived matrix, comprising the steps of: (a) obtaining an adipose tissue;(b) mechanically reducing the size of the adipose tissue;(c) delipidating the adipose tissue by contacting the adipose tissue with a polar organic solvent such that lipids are transfer
1. A method of preparing an adipose tissue-derived matrix, comprising the steps of: (a) obtaining an adipose tissue;(b) mechanically reducing the size of the adipose tissue;(c) delipidating the adipose tissue by contacting the adipose tissue with a polar organic solvent such that lipids are transferred from the adipose tissue to the polar organic solvent, thereby producing a delipidated adipose tissue from which substantially all of the lipids native to the adipose tissue have been removed;(d) decellularizing the delipidated adipose tissue after step (c), thereby producing a delipidated, decellularized adipose tissue which is essentially free of native cells and cellular components. 2. The method claim 1, comprising the further step of separating the adipose tissue from lipids released from the adipose tissue during the mechanically reducing step (b). 3. The method of claim 1, wherein said step of mechanically reducing the size of the adipose tissue includes grinding the adipose tissue with a coarse grinding plate, then grinding the ground adipose tissue with a fine grinding plate. 4. The method of claim 1, wherein said delipidating step includes washing the adipose tissue with a polar organic solvent, wherein the polar solvent includes a substituted hydrocarbon having a number of carbon atoms in the range of two carbon atoms to six carbon atoms, and said substituted hydrocarbon is selected from the group consisting of a chlorinated hydrocarbon, a fluorinated hydrocarbon, an alcohol, an ether, a ketone, an aldehyde, an ester, an organic acid, and combinations thereof. 5. The method of claim 1, wherein said delipidating step includes blending the adipose tissue with a polar organic solvent, Wherein the polar solvent includes a substituted hydrocarbon having a number of carbon atoms in the range of two carbon atoms to six carbon atoms, and said substituted hydrocarbon is selected from the group consisting of a chlorinated hydrocarbon, a fluorinated hydrocarbon, an alcohol, an ether, a ketone, an aldehyde, an ester, an organic acid, and combinations thereof. 6. The method of claim 1, wherein said delipidating step includes homogenizing the adipose tissue with a polar organic solvent, wherein the polar solvent includes a substituted hydrocarbon having a number of carbon atoms in the range of two carbon atoms to six carbon atoms, and said substituted hydrocarbon is selected from the group consisting of a chlorinated hydrocarbon, a fluorinated hydrocarbon, an alcohol, an ether, a ketone, an aldehyde, an ester, an organic acid, and combinations thereof. 7. The method of claim 1, wherein said decellularizing step includes contacting the delipidated adipose tissue with a detergent solution, wherein said detergent is present in said solution at a concentration in a range of from about 0.1% to about 5.0% (w/v). 8. The method of claim 7, wherein the detergent solution includes a deoxycholate salt. 9. The method of claim 7, wherein the detergent solution includes a mixture of water and alcohol. 10. The method of claim 9, wherein the alcohol is present in the detergent solution in an amount in a range of from about 20% to about 40% by volume. 11. The method of claim 1, comprising the further step of soaking the delipidated, decellularized adipose tissue in a disinfectant solution. 12. The method of claim 11, comprising the further step of blending the delipidated, decellularized adipose tissue with the disinfectant solution. 13. The method of claim 11, wherein the disinfectant solution is an acidic solution that includes peracetic acid. 14. The method of claim 11, wherein the disinfectant solution includes an alcohol and a glycol. 15. The method of claim 11, wherein substantially all substances that pose a significant risk of causing an immunogenic response in a patient receiving said adipose tissue-derived matrix are removed from the adipose tissue. 16. The method of claim 11, wherein substantially all native lipids and native nucleic acids are removed from the adipose tissue. 17. The method of claim 1, comprising the further step of contacting the delipidated, decellularized adipose tissue with a solution having an acidic pH so as to form a gel. 18. The method of claim 1, comprising the further step of blending the delipidated, decellularized adipose tissue in a polar liquid, whereby the delipidated, decellularized adipose tissue and the polar liquid form a flowable gel. 19. The method of claim 1, comprising the further steps of drying the delipidated, decellularized adipose tissue, and reducing the dried delipidated, decellularized adipose tissue to a dried particulate form. 20. The method of claim 8, wherein the detergent solution includes a mixture of water and alcohol. 21. The method of claim 12, wherein the disinfectant solution is an acidic solution that includes peracetic acid. 22. The method of claim 12 wherein the disinfectant solution includes an alcohol and a glycol. 23. The method of claim 12, wherein substantially all substances that pose a significant risk of causing an immunogenic response in a patient receiving said adipose tissue-derived matrix are removed from the adipose tissue. 24. The method of claim 12, wherein substantially all native lipids and native nucleic adds are removed from the adipose tissue.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (250)
Livesey, Stephen A.; del Campo, Anthony A.; Nag, Abhijit; Nichols, Ken B.; Griffey, Edward S.; Coleman, Christopher, Acellular dermal matrix and method of use thereof for grafting.
Katz, Adam J.; Llull, Ramon; Futrell, William J.; Hedrick, Marc H.; Benhaim, Prosper; Lorenz, Hermann Peter; Zhu, Min, Adipose-derived stem cells and lattices.
Hedrick, Marc H.; Fraser, John K.; Schulzki, Michael J.; Byrnes, Bobby; Carlson, Grace; Schreiber, Ronda Elizabeth; Wulur, Isabella, Automated methods for isolating and using clinically safe adipose derived regenerative cells.
Abatangelo, Giovanni; Callegaro, Lanfranco, Biological material containing bone marrow stem cells partially or completely differentiated into connective tissue cells and a hyaluronic acid ester matrix.
Brendel Klaus (Tucson AZ) Duhamel Raymond C. (Tucson AZ), Body implants of extracellular matrix and means and methods of making and using such implants.
Abraham Ginger A. ; Carr ; Jr. Robert M. ; Kemp Paul D.,GBX ; Mercer Ryan ; Baker Linda, Chemical treatment, without detergents or enzymes, of tissue to form an acellular, collagenous matrix.
Rosson, Gedge; Elisseeff, Jennifer H.; Nahas, Zayna; Ye, Zhaoyang; Hillel, Alexander, Compositions and methods for implantation of adipose tissue and adipose tissue products.
Boyden, Edward S.; Cook, Daniel B.; Hyde, Roderick A.; Leuthardt, Eric C.; Myhrvold, Nathan P.; Sweeney, Elizabeth A.; Wood, Jr., Lowell L., Compositions and methods for surface abrasion with frozen particles.
Shimko, Daniel Andrew; Noel, Scott P.; Drapeau, Susan J.; Roy, Josee, Compositions for enhancing the viability of animal cells, tissues, and organ explants comprising two low molecular weight PEGs.
Masinaei, Leila; Wolfinbarger, Jr., Lloyd; Chen, Silvia S.; Jones, Alyce Linthurst; Chen, Jingsong, Compositions for repair of defects in tissues, and methods of making the same.
Masinaei, Leila; Wolfinbarger, Jr., Lloyd; Chen, Silvia S.; Jones, Alyce Linthurst; Chen, Jingsong, Compositions for repair of defects in tissues, and methods of making the same.
Mills, C. Randal; Bianchi, John R.; Roberts, Michael R.; Cheung, David T.; Nataraj, Chandrasekaran; Howell, John W., Connective-tissue-based or dermal-tissue-based grafts/implants.
Alchas, Paul G.; Augello, Frank A.; Brooks, Christopher J.; Cutshall, Tony A.; DiPisa, Jr., Joseph A.; Williams, Stuart K.; Gabel, Jonathan B.; Mulhauser, Paul J.; Prais, Wes; Jarrell, Bruce E.; Rose, Endothelial cell procurement and deposition kit.
Williams Stuart K. (Tucson AZ) Jarrell Bruce E. (Tucson AZ), Implant materials, methods of treating the surface of implants with microvascular endothelial cells, and the treated imp.
Williams Stuart K. (Drexell PA) Jarrell Bruce E. (Philadelphia PA), Implantable prosthetic device for implantation into a human patient having a surface treated with microvascular endothel.
Gronthos, Stan; Zannettino, Andrew Christopher William; Simmons, Paul John, Isolation of adult multipotential cells by tissue non-specific alkaline phosphatase.
Shimko, Daniel A.; Noel, Scott; Drapeau, Susan J.; Roy, Josee, Method for enhancing the viability of mammalian cells, tissues and organs using a solution comprising two low molecular weight PEGs.
Livesey Stephen A. (Eltham TX AUX) del Campo Anthony A. (Houston TX) Nag Abhijit (Houston TX) Nichols Ken B. (The Woodlands TX) Coleman Christopher (Houston TX), Method for processing and preserving collagen-based tissues for transplantation.
Franz Rieser CH; Werner Muller CH; Pedro Bittmann CH; Pierre Mainil-Varlet CH; Christoph P. Saager CH, Method for producing cartilage tissue and implants for repairing enchondral and osteochondral defects as well as arrangement for carrying out the method.
Park, Jung-Keug; Yoon, Hee-Hoon; Yoo, Bo-Young; Kim, Young-Jin; Shin, Youn-Ho, Method for the preparation of dermal papilla tissue employing mesenchymal stem cells.
Chu, Benjamin; Hsiao, Benjamin S.; Hadjiargyrou, Michael; Fang, Dufei; Zong, Xinhua; Kim, Kwangsok, Method of cell storage in a delivery system comprising a fibrous matrix.
Williams Stuart K. (Wilmington DE) Jarrell Bruce E. (Philadelphia PA), Method of treating a synthetic naturally occurring surface with a collagen laminate to support microvascular endothelial.
Williams Stuart K. (Drexel Hill PA) Jarrell Bruce E. (Philadelphia PA), Method of treating a synthetic or naturally occuring surface with microvascular endothelial cells, and the treated surfa.
Lacy Paul E. (63 Marshall Pl. St. Louis MO 63119) Scharp David W. (1469 Willow Brook Cove St. Louis MO 63146) Ricordi Camillo (Corso Porta Nuova 10 20121 Milano ITX), Method to isolate clusters of cell subtypes from organs.
Hedrick, Marc H.; Fraser, John K.; Riley, Susan Lynn; Schreiber, Ronda Elizabeth, Methods of using regenerative cells in the treatment of musculoskeletal disorders.
Hampson, Brian; Goltry, Kristin; Smith, Douglas M.; Rowley, Jonathan A.; Venturi, Naia, Mixed cell populations for tissue repair and separation technique for cell processing.
Kang, Kyung Sun; Ra, Jeong Chan; Park, Jung Ran, Multipotent stem cells derived from human adipose tissue and cellular therapeutic agents comprising the same.
Mills, C. Randal; Bianchi, John R.; Roberts, Michael R.; Cheung, David T.; Nataraj, Chandrasekaran; Howell, Jr., John W., Muscle-based grafts/implants.
Tayot Jean-Louis (La Tour de Salvagny FRX) Tardy Michel (Lyons FRX), New preparation of placenta collagen, their extraction method and their applications.
Ke,HuaZhu; Thompson,David D., Pharmaceutical compositions, kits and methods comprising combinations of estrogen agonists/antagonists, estrogens and progestins.
Wolfinbarger, Jr., Lloyd; Lange, Perry; Linhurst, Alyce; Moore, Eric; Nolf, Barry, Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced.
Wolfinbarger, Jr., Lloyd; Lange, Perry; Linthurst Jones, Alyce; Moore, Eric; Nolf, Barry, Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced.
Wolfinbarger, Jr.,Lloyd; Lange,Perry; Linhurst Jones,Alyce; Moore,Eric; Nolf,Barry, Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced.
Chancellor, Michael B.; Huard, Johnny; Capelli, Christopher; Chung, Steve; Sacks, Michael S., Rapid preparation of stem cell matrices for use in tissue and organ treatment and repair.
Naughton, Gail K.; Ziegler, Frank; Baumgartner, Mark; Nickey, Kyle, Repair and/or regeneration of cells with a composition produced by culturing fibroblast cells under hypoxic conditions.
Niwa,Hideo; Fukuchi,Takeshi; Shimizu,Ichiro; Sato,Masao; Nishi,Akiko; Yamashita,Kenji; Kaneko,Tadashi; Ohgushi,Hajime; Hattori,Koji; Uematsu,Kota, Support for tissue regeneration and process for producing the same.
Hedrick, Marc H.; Fraser, John K.; Schulzki, Michael J.; Byrnes, Bobby; Carlson, Grace; Schreiber, Ronda; Wulur, Isabella, Systems and methods for isolating and using clinically safe adipose derived regenerative cells.
Hedrick, Marc H.; Fraser, John K.; Schulzki, Michael J.; Byrnes, Bobby; Carlson, Grace; Shanahan, Robert K., Systems and methods for separating and concentrating adipose derived stem cells from tissue.
Hamby, Joseph; Walsh, Steven; Heacox, Al; Goldstein, Steven; Gale, David; Arnold, Stacy; Updegrove, Ann Sands, Tissue implants for implantation and methods for preparing the same.
Halvorsen, Yuan-Di C.; Wilkison, William O.; Gimble, Jeffrey Martin, Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair.
Halvorsen,Yuan Di C.; Wilkison,William O.; Gimble,Jeffrey Martin, Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair.
Yuan-Di C. Halvorsen ; William O. Wilkison ; Jeffrey Martin Gimble, Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair.
Fernández Miguel, María Gema; González de la Peña, Manuel Angel; García Castro, Rosa Ana; García Arranz, Mariano; García Olmo, Damián, Use of adipose tissue-derived stromal stem cells in treating fistula.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.