[미국특허]
Methods and apparatus for particle aggregation using acoustic standing waves
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C02F-001/36
C12M-001/00
C02F-001/00
B01D-021/28
B01D-021/34
B01D-015/08
C12N-013/00
B06B-001/06
C12M-001/42
C02F-101/32
출원번호
US-0397326
(2017-01-03)
등록번호
US-10106770
(2018-10-23)
발명자
/ 주소
Presz, Jr., Walter M.
Chitale, Kedar
Lipkens, Bart
출원인 / 주소
FloDesign Sonics, Inc.
대리인 / 주소
Klein, Esq., Rick
인용정보
피인용 횟수 :
0인용 특허 :
125
초록▼
Methods for generating particulate clusters and nodal trapping lines having desired widths are disclosed. The devices include an acoustic chamber having an inlet and an outlet. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave that generates particulate cluster
Methods for generating particulate clusters and nodal trapping lines having desired widths are disclosed. The devices include an acoustic chamber having an inlet and an outlet. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave that generates particulate clusters separated by a channel of fluid running therebetween and creates nodal trapping lines. The frequency of the multi-dimensional acoustic standing wave can be selectively tuned so as to selectively control at least one of (a) a width of each particulate cluster, or (b) a width of each channel of fluid. The frequency of the multi-dimensional acoustic standing wave can also be selectively tuned so as to selectively control the width of each nodal trapping line. Also disclosed are particulate clusters separated by a channel of fluid, wherein a ratio of the widths of the particulate clusters and the channel of fluid can be varied as desired.
대표청구항▼
1. A method of generating particulate clusters, the method comprising: flowing a mixture of a host fluid and particulate through an acoustophoretic device, the device comprising: a housing that defines an acoustic chamber;at least one outlet from the acoustic chamber;at least one inlet to the acoust
1. A method of generating particulate clusters, the method comprising: flowing a mixture of a host fluid and particulate through an acoustophoretic device, the device comprising: a housing that defines an acoustic chamber;at least one outlet from the acoustic chamber;at least one inlet to the acoustic chamber; andat least one ultrasonic transducer coupled to the acoustic chamber and at least one reflector coupled to the acoustic chamber opposite the at least one ultrasonic transducer, the at least one ultrasonic transducer including a piezoelectric material; anddriving the at least one ultrasonic transducer to create a multi-dimensional acoustic standing wave in the acoustic chamber to generate at least a first particulate cluster and a second particulate cluster that are separated by a fluid channel running therebetween; andselectively driving the at least one ultrasonic transducer so as to selectively control either (a) a dimension of each particulate cluster, or (b) a dimension of the fluid channel. 2. The method of claim 1, further comprising driving the at least one ultrasonic transducer with a voltage signal, wherein the frequency of the voltage signal is selectively tuned such that a height of each of the first and second particulate clusters is from about 150 micrometers to about 1200 micrometers. 3. The method of claim 1, further comprising driving the at least one ultrasonic transducer with a voltage signal, wherein the frequency of the voltage signal is selectively tuned such that the fluid channel has a height of from about 50 micrometers to about 500 micrometers. 4. The method of claim 1, further comprising driving the at least one ultrasonic transducer with a voltage signal, wherein the frequency of the voltage signal is selectively tuned such that a ratio of the height of the first and second particulate clusters to the height of the fluid channel is from about 1:1 to about 5:1. 5. The method of claim 1, further comprising driving the at least one ultrasonic transducer with a voltage signal, wherein the frequency of the voltage signal is selectively tuned such that: the first and second particulate clusters are each about 150 micrometers to about 1200 micrometers in height; andthe fluid channel has a height of from about 50 micrometers to about 500 micrometers; anda ratio of the height of the first and second particulate clusters to the height of the fluid channel is from about 1:1 to about 5:1. 6. The method of claim 1, further comprising driving the at least one ultrasonic transducer with a voltage signal, wherein the frequency of the voltage signal is selectively tuned such that: the first and second particulate clusters are each about 200 micrometers to about 600 micrometers in height; andthe fluid channel has a height of from about 100 micrometers to about 250 micrometers; anda ratio of the height of the first and second particulate clusters to the height of the fluid channel is from about 1:1 to about 5:1. 7. The method of claim 1, wherein the particulates are selected from the group consisting of Chinese hamster ovary (CHO) cells, NS0 hybridoma cells, baby hamster kidney (BHK) cells, human cells, T cells, B cells, NK cells, algae, bacteria, viruses, or microcarriers. 8. The method of claim 1, wherein the acoustophoretic device is part of a filter train. 9. The method of claim 1, further comprising collecting the particulate clusters and sending the particulate clusters through at least one additional downstream filtration stage. 10. The method of claim 1, further comprising separating the particulate clusters from the host fluid to obtain a clarified host fluid, and sending the clarified host fluid through at least one additional downstream filtration stage. 11. A method of controlling particulate clusters, the method comprising: providing a mixture of a host fluid and particulate to an acoustophoretic device, the device comprising: an acoustic chamber; andat least one ultrasonic transducer coupled to the acoustic chamber and at least one reflector coupled to the acoustic chamber opposite the at least one ultrasonic transducer, the at least one ultrasonic transducer including a piezoelectric material;driving the at least one ultrasonic transducer to generate a multi-dimensional acoustic standing wave in the acoustic chamber to generate at least a first particulate cluster in a first nodal trapping line and a second particulate cluster in a second nodal trapping line that are spaced from each other in a direction of gravity; andselectively driving the at least one ultrasonic transducer such that one of the first particulate cluster or the second particulate cluster bleeds material into the other of the first particulate cluster or the second particulate cluster. 12. A method of controlling particulate clusters, the method comprising: providing a mixture of a host fluid and particulate to an acoustophoretic device, the device comprising: an acoustic chamber; andat least one ultrasonic transducer coupled to the acoustic chamber and at least one reflector coupled to the acoustic chamber opposite the at least one ultrasonic transducer, the at least one ultrasonic transducer including a piezoelectric material;driving the at least one ultrasonic transducer to generate a multi-dimensional acoustic standing wave in the acoustic chamber to generate at least a first particulate cluster and a second particulate cluster that are separated by a fluid channel running therebetween; andselectively driving the at least one ultrasonic transducer such that the first particulate cluster and the second particulate cluster are maintained in and do not leave the multi-dimensional acoustic standing wave.
Trampler Felix (Hinterbruehl ATX) Piret James M. (Vancouver CAX) Sonderhoff Stefan A. (Vancouver CAX) Kilburn Douglas G. (Vancouver CAX), Acoustic filter for separating and recycling suspended particles.
Bolleman Brent (4021 West 30th Avenue Vancouver ; British Columbia CAX V6S 1X4) Dunwoody A. Bruce (9571 Pickering Drive Richmond ; British Columbia CAX V7E 5A3), Acoustic liquid processing device.
Lipkens, Bart; Barnes, Jason; Mealey, Dane; Presz, Jr., Walter M.; Kowalski, III, Stanley; Masi, Louis; Kennedy, III, Thomas J.; McCarthy, Brian; Ross-Johnsrud, Ben, Acoustophoresis device with modular components.
Lipkens, Bart; Dionne, Jason; Presz, Jr., Walter M.; Kennedy, III, Thomas J., Acoustophoretic separation technology using multi-dimensional standing waves.
Sliwa ; Jr. John W. (Palo Alto CA) Curley Michael G. (Belmont CA) Mullen Donald R. (Fremont CA) Plugge Jay S. (Mountain View CA) Lyon Richard A. (Palo Alto CA), Active thermal control of ultrasound transducers.
Lipkens, Bart; Masi, Louis; Kowalski, III, Stanley; Presz, Jr., Walter M.; Dionne, Jason; Dutra, Brian; Mercado, Ari; Kennedy, III, Thomas J.; Martin, Arthur, Bioreactor using acoustic standing waves.
Bernou Jean-Louis (Fresnes FRX) Dufau Frederic (La Celle St Cloud FRX) Geahel Isabelle (St Germain En Laye FRX) Hache Jean (Voisins Le Bretonneux FRX), Filtration apparatus comprising an ultrasonic cleaning device and corresponding cleaning process.
Wada, H. Garrett; Kopf-Sill, Anne R.; Alajoki, Marja Liisa; Parce, J. Wallace; Wang, Benjamin N.; Chow, Andrea W.; Dubrow, Robert S.; Yurkovetsky, Yevgeny; Farinas, Javier Anibal, Focusing of microparticles in microfluidic systems.
Becker, Frederick F.; Gascoyne, Peter R. C.; Huang, Ying; Wang, Xiao-Bo; Yang, Jun, Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation.
Redeker,Fred C.; Boyd,John M.; Parks,John, Method and apparatus for semiconductor wafer cleaning using high-frequency acoustic energy with supercritical fluid.
Peterson Stephen C. (Salt Lake City UT) Brimhall Owen D. (Salt Lake City UT) McLaughlin Thomas J. (Salt Lake City UT) Baker Charles D. (Lehi UT) Sparks Sam L. (Alpine UT), Methods and apparatus for moving and separating materials exhibiting different physical properties.
Peterson Stephen C. (al of Salt Lake City UT) Brimhall Owen D. (al of Salt Lake City UT) McLaughlin Thomas J. (al of Salt Lake City UT) Baker Charles D. (Lehi UT) Sparks Sam L. (Alpine UT), Methods and apparatus for moving and separating materials exhibiting different physical properties.
Vivek, Vibhu; Hadimioglu, Babur; Dadi, Ratnakar, Methods and systems to form high efficiency and uniform fresnel lens arrays for ultrasonic liquid manipulation.
Trampler Felix (Hinterbruehl ATX) Benes Ewald (Biedermannsdorf ATX) Burger Wolfgang (Vienna ATX) Grschl Martin (Vienna ATX), Multilayered piezoelectric resonator for the separation of suspended particles.
Stuckart Wolfgang (Keinergasse 17/14 A - 1030 Wien ATX), Process for the separation of substances from a liquid and device for effecting such a process.
Lipkens, Bart; Dionne, Jason; Mercado, Ari; Dutra, Brian; Presz, Jr., Walter M.; Kennedy, III, Thomas J.; Masi, Louis, Separation of multi-component fluid through ultrasonic acoustophoresis.
Mariella, Jr., Raymond P.; Dougherty, George M.; Dzenitis, John M.; Miles, Robin R.; Clague, David S., Systems and methods for separating particles and/or substances from a sample fluid.
King Karl L. (Brown Deer WI) Weiss Bruce W. (Whitefish Bay WI) Endl Robert W. (Johnson Creek WI), Turbidimeter having a baffle assembly for removing entrained gas.
Lynnworth Lawrence C. (Waltham MA) Seger John L. (Waltham MA) Bradshaw James E. (Tyngsboro MA), Ultrasonic system for measuring fluid impedance or liquid level.
Lipkens, Bart; Mitchell, Eric; Carmichael, Joey; Mealey, Dane; Dionne, Jason, Ultrasound and acoustophoresis for collection and processing of oleaginous microorganisms.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.