Solid inoculum carried in a superabsorbent agro-pellet with a variable volume, operating in a natural minifermenter, and production method
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C05F-011/08
C05G-003/00
C12N-001/04
C12N-001/14
C12N-001/20
C12N-001/22
C12N-011/02
C12N-011/10
C12N-011/12
출원번호
US-0906024
(2014-07-22)
등록번호
US-10131586
(2018-11-20)
우선권정보
FR-13 01727 (2013-07-19)
국제출원번호
PCT/FR2014/000173
(2014-07-22)
국제공개번호
WO2015/007964
(2015-01-22)
발명자
/ 주소
Chelle, Rene
Makoumbou, Urbain
출원인 / 주소
AB7 Innovations S.A.S.U.
대리인 / 주소
Klauber & Jackson LLC
인용정보
피인용 횟수 :
0인용 특허 :
2
초록▼
The invention relates to a solid inoculum carried in a superabsorbent agro-pellet with a variable volume, operating in a natural mini fermenter preloaded with an anhydrous nutritive medium, encouraging rapid regrowth of the viable micro-organisms and the continuous multiplication thereof. The liquid
The invention relates to a solid inoculum carried in a superabsorbent agro-pellet with a variable volume, operating in a natural mini fermenter preloaded with an anhydrous nutritive medium, encouraging rapid regrowth of the viable micro-organisms and the continuous multiplication thereof. The liquid inoculum incorporated into the carrier at a low density of microbial cells is preferably comprised between 1/500e and 1/10e of the density recommended by the norm for a liquid inoculum of the same strain. The bioaugmentation of the microbial population is between 104 and 106 additional microbial cells. The solid inoculum according to the invention allows the preservation of the microorganisms and the handling thereof in advantageous conditions. It is not exposed to soil leaching caused by water. Said inoculum is used for the biological treatment of crops for the development and/or the preservation thereof from parasite attacks.
대표청구항▼
1. Solid inoculum carrier made of a superabsorbent biodegradable plasticized composite matrix agro-pellet capable of absorbing water or an aqueous solution up to 500% of its own weight, and having a porous structure, comprising: a) a mixture of biopolymer comprising i) from 55% to 60% by weight of a
1. Solid inoculum carrier made of a superabsorbent biodegradable plasticized composite matrix agro-pellet capable of absorbing water or an aqueous solution up to 500% of its own weight, and having a porous structure, comprising: a) a mixture of biopolymer comprising i) from 55% to 60% by weight of a protein biopolymer comprising globulin, albumin, prolamin and fibre, chosen from a by-product of a plant matter comprising protein, selected from the group consisting of sunflower, soy, colza, alfalfa, and linseed plant matter;ii) from 40% to 45% by weight of a polysaccharide biopolymer comprising starch, hemicellulose and pectin, selected from the group consisting of grain flour and sugar beet pulp,b) from 5% to 40% by weight of a solid mixture of natural hyaline or hydrocyst cell tissue selected from the group consisting of sphagnum and Carduus thistle;c) an anhydrous nutritive medium; andd) an inoculum of microorganisms having a density of 104 CFU/g to 2×106 CFU/g of the microorganisms; wherein said agro-pellet is capable of increasing in volume up to a naturally limited volume to avoid percolation of said water or an aqueous solution and prevent leaching of said anhydrous nutritive medium, wherein said agro-pellet performs simultaneously the functions of support and fermenter, enabling the multiplication within the agro-pellet of the microorganisms of said inoculum. 2. Solid inoculum carrier according to claim 1, wherein said agro-pellet incorporates said anhydrous nutritive medium at a level between 25% and 500% of its own weight. 3. Solid inoculum carrier according to claim 1, wherein the anhydrous nutritive medium contained in the agro-pellet is enabled to be dissolved partially and progressively by rain or irrigation water with no risk of leaching. 4. Solid inoculum carrier according to claim 1, wherein said agro-pellet is enabled for partial and progressive dissolution of said nutritional medium upon rehydration, to promote sustained increase in continuous multiplication of the microorganisms therein, including when said agro-pellet is interred. 5. Solid inoculum carrier according to claim 1, wherein the agro-pellet has a dry matter content of between 92% and 95% its weight. 6. Solid inoculum carrier according to claim 1, wherein said agro-pellet is enabled to vary in volume without disintegrating by swelling via water or aqueous solution absorption and retracting by releasing these said liquids without disintegrating. 7. Solid inoculum carrier according to claim 1, wherein the microorganisms are a mycorrhizal fungus or a rhizobacteria. 8. Solid inoculum carrier according to claim 7, wherein the microorganisms are an Azotobacter spp., an Acidovorax facilis, a Flavobacterium spp., a Pseudomonas spp., a Rhodococcus rhodochrous, a Bacillus subtilis, a Bacillus chitinoporus, a Bacillus laterosporus, a Bacillus thuringiensis, a Saccharopolyspora spinosa, a Meterhizium anisopliae or a Beauvaria bassiana. 9. Solid inoculum carrier according to claim 1, wherein the superabsorbent agro-pellet has a dry matter content of between 92% and 95% its weight. 10. Solid inoculum carrier according to claim 4, wherein said superabsorbent agro-pellet is enabled to vary in volume without disintegrating by swelling via water or aqueous solution absorption and retracting by releasing these said liquids without disintegrating. 11. Solid inoculum carrier according to claim 1, wherein said superabsorbent agro-pellet is enabled to vary in volume without disintegrating by swelling via water or aqueous solution absorption and retracting by releasing these said liquids without disintegrating. 12. Method for production of a solid inoculum carrier according to claim 1 consisting of the following steps: a) incorporating the liquid nutritive medium adapted to a strain to inoculate into: a first support comprising: a) a mixture of biopolymer comprising i) from 55% to 60% by weight of a protein biopolymer comprising globulin, albumin, prolamin and fibre, chosen from a by-product of a plant matter comprising protein, selected from the group consisting of sunflower, soy, colza, alfalfa, and linseed plant matter;ii) from 40% to 45% by weight of a polysaccharide biopolymer comprising starch, hemicellulose and pectin, selected from the group consisting of grain flour and sugar beet pulp, andb) from 5% to 40% by weight of a solid mixture of natural hyaline or hydrocyst cell tissue selected from the group consisting of sphagnum and Carduus thistle,at a level between about 25% and about 500% of its weight to obtain a second support;b) dehydrating the second support obtained in a) between 40° C. and 55° C. until the dry matter content reaches a level between 90% and 95% by weight, to obtain a dry support;c) sterilising the dry support obtained in b) in an air-tight container to obtain a sterile support;d) incorporating a liquid inoculum of microorganism into the sterile support obtained in c) to obtain a microbial density varying between 104 CFU/g to 2×106 CFU/g to obtain a loaded support;e) dehydrating the loaded support obtained in d) by dry heat at a temperature between 35° C. and 40° C. to obtain a solid inoculum carrier having a dry matter content between 90% and 95% by weight;f) packaging the solid inoculum carrier obtained in e) so as to avoid contact with humidity. 13. Method according to claim 12, wherein the incorporation of the liquid nutritive medium into said support is performed by sprinkling, spraying or rapid soaking. 14. Method according to claim 12, wherein the nutritive medium incorporated into the support comprises Yeast extract, mannitol, MgSO4, 7H2O, and NaCl. 15. Method according to claim 12, wherein the liquid inoculum of microorganisms is incorporated into the support by sprinkling or spraying. 16. Method according to claim 13, wherein the support is sterilized in an autoclave, by irradiation, or by tyndallisation.
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이 특허에 인용된 특허 (2)
Kremer Douglas A. (Minnetonka MN) Peterson David E. (Plymouth MN), Method for coating bareroot plants with a live microbial colony.
Weaver Mary Ollidene (Peoria IL) Bagley Edward B. (Morton IL) Fanta George F. (Peoria IL) Doane William M. (Morton IL), Method of reducing water content of emulsions, suspensions, and dispersions with highly absorbent starch-containing poly.
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