초록 ▼

A nucleic acid amplification device includes: an introduction unit into which a reaction solution including a target nucleic acid is introduced; a nucleic acid amplification reaction section in which at least two temperature zones of different temperature are present, for amplifying the target nucle

A nucleic acid amplification device includes: an introduction unit into which a reaction solution including a target nucleic acid is introduced; a nucleic acid amplification reaction section in which at least two temperature zones of different temperature are present, for amplifying the target nucleic acid included in the reaction solution introduced into the introduction unit; and a channel arranged to pass back and forth or in cyclic fashion through the at least two temperature zones, and having a capillary force transport mechanism for feeding the reaction solution by capillary force.

대표청구항 ▼

1. A nucleic acid amplification device comprising: an introduction unit into which a reaction solution including a target nucleic acid is introduced;a nucleic acid amplification reaction section including at least two temperature zones of different temperature, for amplifying the target nucleic acid

1. A nucleic acid amplification device comprising: an introduction unit into which a reaction solution including a target nucleic acid is introduced;a nucleic acid amplification reaction section including at least two temperature zones of different temperature, for amplifying the target nucleic acid included in the reaction solution introduced into the introduction unit;a channel arranged to pass back and forth or in cyclic fashion through the at least two temperature zones, and having a capillary force transport mechanism for feeding the reaction solution by capillary force;a fed solution retention section for retaining the reaction solution; anda discharge unit configured to discharge the reaction solution including the target nucleic acid after amplification,wherein the channel includes a first channel disposed in the nucleic acid amplification reaction section and a second channel disposed in the fed solution retention section,a first end of the second channel is connected to the first channel,a second end of the second channel is connected to the discharge unit,a volumetric capacity of the second channel is 10% or more of a total volumetric capacity of the channel, andthe second channel retains the reaction solution fed from the first channel. 2. The nucleic acid amplification device according to claim 1, wherein the channel is connected to an external space only in the introduction unit and the discharge unit. 3. The nucleic acid amplification device according to claim 1, wherein the channel has, as the capillary force transport mechanism, a wall surface that is a hydrophilic surface and has an acute contact angle. 4. The nucleic acid amplification device according to claim 3, wherein wall surfaces around an entire perimeter of the channel in a cross section perpendicular to a feeding direction of the reaction solution are hydrophilic surfaces. 5. The nucleic acid amplification device according to claim 3, wherein the hydrophilic surface of the channel is a surface of a hydrophilic film provided in the channel. 6. The nucleic acid amplification device according to claim 5, wherein the hydrophilic film comprises a material including a hydrophilic group and a hydrophobic group. 7. The nucleic acid amplification device according to claim 6, wherein the hydrophilic film comprises a surfactant. 8. The nucleic acid amplification device according to claim 7, wherein the surfactant is a non-ionic surfactant. 9. The nucleic acid amplification device according to claim 1, wherein the introduction unit comprises a plurality of introduction units,the nucleic acid amplification device further comprises a mixer disposed between the nucleic acid amplification reaction section and the plurality of introduction units,the plurality of introduction units are connected as one in the mixer via introduction channels corresponding to the plurality of introduction units, anda plurality of solutions introduced via the plurality of introduction units mix together in the mixer to produce the reaction solution. 10. The nucleic acid amplification device according to claim 1, wherein the channel includes, at least in the nucleic acid amplification reaction section, a region having a cross-sectional area that decreases in the feeding direction. 11. A nucleic acid amplification apparatus comprising: the nucleic acid amplification device according to claim 1; anda temperature controller configured to control a temperature of the nucleic acid amplification reaction section. 12. The nucleic acid amplification apparatus according to claim 11, further comprising a detector configured to detect nucleic acid amplification of the target nucleic acid. 13. The nucleic acid amplification apparatus according to claim 12, wherein the detector includes:a light output configured to output light for irradiating the nucleic acid amplification device; anda light receptor configured to receive reflected light of the light irradiating the nucleic acid amplification device. 14. The nucleic acid amplification apparatus according to claim 13, wherein the detector further includes an optical scanner configured to scan the light over the channel of the nucleic acid amplification device. 15. A nucleic acid amplification method for amplifying a target nucleic acid using a nucleic acid amplification device, the nucleic acid amplification method comprising: introducing the target nucleic acid and a reagent for amplifying the target nucleic acid into an introduction unit of the nucleic acid amplification device; andas a reaction solution including the target nucleic acid and the reagent is fed by capillary force, amplifying the target nucleic acid included in the reaction solution,wherein the nucleic acid amplification device includes a fed solution retention section for retaining the reaction solution, the fed solution retention section including a channel including a channel portion having a volumetric capacity that is 10% or more of a total volumetric capacity of the channel; and a discharge unit that discharges the reaction solution including the target nucleic acid after amplification, andwhen a fluid front of the reaction solution fed by capillary force reaches the discharge unit, introduction of a solution including the target nucleic acid into the introduction unit is interrupted. 16. The nucleic acid amplification method according to claim 15, wherein in the introducing, a premixed solution of (i) a solution including the target nucleic acid and (ii) the reagent is introduced into the nucleic acid amplification device as the reaction solution. 17. The nucleic acid amplification method according to claim 15, wherein in the introducing, a first solution including the target nucleic acid and a second solution including the reagent are separately introduced into the nucleic acid amplification device, andin the amplifying, as a solution of the first solution and the second solution mixed together in the nucleic acid amplification device is fed by capillary force as the reaction solution, the target nucleic acid included in the reaction solution is amplified. 18. The nucleic acid amplification method according to claim 15, wherein in the amplifying, the target nucleic acid included in the reaction solution is amplified by subjecting the reaction solution to cyclic temperature changes. 19. The nucleic acid amplification apparatus according to claim 1, wherein the second channel includes a meandering path. 20. The nucleic acid amplification method according to claim 15, the channel portion including a meandering path.