Coating process for microfluidic sample arrays
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
B01J-019/00
B01L-003/00
B01L-003/02
출원번호
US-0300100
(2014-06-09)
등록번호
US-10213761
(2019-02-26)
발명자
/ 주소
Brenan, Colin J. H.
Cho, Jamie
Garcia, Javier
Hess, Robert
Kanigan, Tanya S.
Katz, Arrin
Kim, Namyong
Linton, John
Srivastava, Shailesh
Yoder, Karl
출원인 / 주소
Life Technologies Corporation
대리인 / 주소
Weber, David
인용정보
피인용 횟수 :
0인용 특허 :
124
초록▼
A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interi
A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one exterior surface that is selectively coated with an optionally dissolvable coating agent physisorbed to at least one interior surface, wherein the optionally dissolvable coating agent comprises a coating agent and a first component for the plurality of specified reactions. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. A first one of the dispenser array and the microfluidic array is movable in relation to the frame, and the other of the first one of the dispenser array and the microfluidic array is fixed relative to the frame. Quantities related to a vector displacement from the alignment position to a fixed position on the one of the dispenser array and the microfluidic array is determined. The quantities thus determined are used to guide positioning of the dispenser array relative to the microfluidic array.
대표청구항▼
1. A method for differentially coating a substrate, the method comprising: supplying a substrate comprising an external surface and a plurality of through-hole channels each comprising an interior surface in communication with the exterior surface;applying a first coating agent simultaneously to bot
1. A method for differentially coating a substrate, the method comprising: supplying a substrate comprising an external surface and a plurality of through-hole channels each comprising an interior surface in communication with the exterior surface;applying a first coating agent simultaneously to both the exterior surface and to at least one of the interior surfaces;selectively oxidizing the at least one interior surface with an oxidizing agent;after selectively oxidizing, selectively modifying the first coating agent applied to the at least one interior surface with a first modifying agent such that, after modifying, the at least one interior surface has a first specified physicochemical property that differs with respect to a specified physicochemical property of the external surface; andapplying a second modifying agent to the at least one interior surface;wherein at least one of (1) the first coating agent comprises a vinyl terminated compound or (2) the first and second modifying agents each comprise a polyethylene glycol. 2. A method for differentially coating a substrate having an exterior surface and a plurality of through-hole channels each comprising an interior surface in communication with the exterior surface, the method comprising: creating a coated surface by applying a first coating agent simultaneously to both the exterior surface and to at least one of the interior surfaces;selectively oxidizing the at least one interior surface with an oxidizing agent; andafter selectively oxidizing, selectively modifying the first coating agent with a first modifying agent such that the coated surface comprises a modified surface and a non-modified surface, the modified surface comprising the at least one interior surface and having a first specified physicochemical property, the non-modified surface comprising a second specified physicochemical property that differs with respect to the first specified physicochemical property;wherein at least one of (1) the first coating agent comprises a vinyl terminated compound or (2) the first and second modifying agents each comprise a polyethylene glycol. 3. A method for differentially coating a substrate having an exterior surface and a plurality of through-hole channels each comprising an interior surface in communication with the exterior surface, the method comprising: creating a coated surface by applying a first coating agent simultaneously to both the exterior surface and to at least one of the interior surfaces;selectively oxidizing the at least one interior surface with an oxidizing agent; andafter selectively oxidizing, selectively modifying the first coating agent with a first modifying agent such that the coated surface comprises a modified surface and a non-modified surface, the modified surface comprising the at least one interior surface, the unmodified surface comprising exterior surface;wherein at least one of (1) the first coating agent comprises a vinyl terminated compound or (2) the first and second modifying agents each comprise a polyethylene glycol. 4. The method of claim 1, wherein the first coating agent comprises a vinyl terminated compound and the modifying agent comprises a polyethylene glycol. 5. The method of claim 1, further comprising, after applying the second modifying agent, applying a film by vapor deposition onto the external surface, wherein the second modifying agent protects the plurality of through-hole channels. 6. The method of claim 5, further comprising removing the second modifying agent, wherein the at least one interior surfaces comprises a hydrophilic surface and the exterior surface comprises a hydrophobic surface. 7. The method of claim 6, wherein the hydrophobic surface comprises a perfluorosilane film. 8. The method of claim 2, wherein the first coating agent comprises a vinyl terminated compound and the modifying agent comprises a polyethylene glycol. 9. The method of claim 2, further comprising applying a second modifying agent to the at least one interior surface. 10. The method of claim 9, further comprising, after applying the second modifying agent, applying a film by vapor deposition onto the external surface, wherein the second modifying agent protects the plurality of through-hole channels. 11. The method of claim 9, further comprising removing the second modifying agent, wherein the at least one interior surfaces comprises a hydrophilic surface and the exterior surface comprises a hydrophobic surface. 12. The method of claim 11, wherein the exterior surface comprises a perfluorosilane film. 13. The method of claim 3, wherein the first coating agent comprises a vinyl terminated compound and the modifying agent comprises a polyethylene glycol. 14. The method of claim 3, further comprising applying a second modifying agent to the at least one interior surface. 15. The method of claim 14, further comprising, after applying the second modifying agent, applying a film by vapor deposition onto the external surface, wherein the second modifying agent protects the plurality of through-hole channels. 16. The method of claim 14, further comprising removing the second modifying agent, wherein the at least one interior surfaces comprises a hydrophilic surface and the exterior surface comprises a hydrophobic surface. 17. The method of claim 16, wherein the exterior surface comprises a perfluorosilane film.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (124)
Heller Michael J. (Encinitas CA) Tu Eugene (San Diego CA), Active programmable electronic devices for molecular biological analysis and diagnostics.
Hess, Robert A.; Linton, John; Kanigan, Tanya S.; Brenan, Colin; Ozbal, Can, Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof.
Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Array of oligonucleotides on a solid substrate.
Roberts David A. (Carlsbad CA) Garg Diwakar (Macungie PA) Lagendijk Andr (Oceanside CA) Hochberg Arthur K. (Solana Beach CA) Fine Stephen M. (Far Hill NJ), Deposition of tungsten films from mixtures of tungsten hexafluoride organohydrosilanes and hydrogen.
J. Fraser Glickman ; James Inglese ; Bassam Damaj, Fluorescent capture assay for kinase activity employing anti-phosphotyrosine antibodies as capture and detection agents.
Arentzen Rene (Wilmington DE) Jadhav Prabhakar K. (Wilmington DE) Kobos Robert K. (Wilmington DE) Smart Bruce E. (Wilmington DE), Immobilization of biomolecules on a fluorocarbon surface with a poly(fluoroalkyl) sugar reagent.
Cassin Peter J. ; Harootunian Alec Tate ; Pham Andrew A. ; Tsien Roger Y., Low background multi-well plates with greater than 864 wells for fluorescence measurements of biological and biochemic.
Tiffany Thomas (E. 1305 56th Spokane WA 99223) Weyrauch Bruce (N. 13707 Coman Rd. Newman Lake WA 99025) Thayer Phillip (N. 8617 Seven Mile Rd. Nine Mile Falls WA 99026), Method and apparatus for conducting multiple chemical assays.
Schleifer Arthur ; Caren Michael P. ; Leonard Leslie A. ; Hotz Charles Z. ; Perbost Michel G. M., Method and apparatus for fabricating replicate arrays of nucleic acid molecules.
Thomson Kenneth S. (1282 S. 164th St. Omaha NE 68130) Thomson Susan A. (1282 S. 164th St. Omaha NE 68130), Method and apparatus for performing 3-dimensional antibiotic susceptibility tests.
Sutton Richard Calvin ; Ponticello Ignazio Salvatore ; Cummins Thomas Joseph ; Zander Dennis Roland ; Donish William Harold ; Chen Paul Hong-Dze ; Findlay John Bruce, Method for nucleic acid amplification and detection using adhered probes.
Roser Bruce J. (Balsham GB2) Colaco Camilo (Trumpington GB2), Method of preserving agarose gel structure during dehydration by adding a non-reducing glycoside of a straight-chain sug.
Lalouel Jean-Marc (Salt Lake City UT) Jeunemaitre Xavier (Paris UT FRX) Lifton Richard P. (Salt Lake City UT) Soubrier Florant (Paris FRX) Kotelevtsev Youri (Edinburgh GBX) Corval Pierre (Paris FRX), Methods to determine predisposition to hypertension and association of variant angiotensinogen gene and hypertension.
Bowers William F. (Topsfield MA) Fulton Scott P. (Brookline MA) Thompson Judith A. (Marblehead MA) Donofrio David M. (Rockport MA) Ivie Kert F. (Spruce Creek PA), Microfiltration apparatus with radially spaced nozzles.
Heller Michael J. (Encinitas CA) Tu Eugene (San Diego CA) Butler William F. (Carlsbad CA), Molecular biological diagnostic systems including electrodes.
Gavet Louis (Lyon FRX) Chatelin Roger (Lissieu Par FRX) Sotton Michel (St Cyr Au Mont D\Or FRX) Guigal Robert (Lyons FRX) Laurent Philippe (Oullins FRX), One-use device for biological tests.
Bensimon David,FRX ; Bensimon Aaron,FRX ; Heslot Fran.cedilla.ois,FRX, Process for aligning, adhering and stretching nucleic acid strands on a support surface by passage through a meniscus.
Schubert Klaus (Karlsruhe DEX) Bier Wilhelm (Eggenstein-Leopoldshafen DEX) Linder Gerd (Karlsruhe DEX) Schmid Peter (Munich DEX) Bichler Peter (Schondorf DEX) Brunner Winfried (Unterhaching DEX) Simo, Process for manufacturing fine-structured bodies.
Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Synthesis and screening of immobilized oligonucleotide arrays.
Campbell David A. ; Antonenko Valery V. ; Selick Harold E., System and method for solid-phase parallel synthesis of a combinatorial collection of compounds.
Stylli Chari ; Beckey Samuel S. ; Shumate Christopher Bentley ; Coassin Peter J., Systems and methods for rapidly identifying useful chemicals in liquid samples.
Atwood John G. (West Redding CT) Mossa Albert C. (Trumbull CT) Goven Lisa M. (Bridgeport CT) Williams Fenton (Brookfield CT) Woudenberg Timothy M. (Bethel CT) Margoulies Marcel (Scarsdale NY) Ragusa , Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control.
Atwood John G. (West Redding CT) Mossa Albert C. (Trumbull CT) Goven Lisa M. (Bridgeport CT) Williams Fenton (Brookfield CT) Woudenberg Timothy M. (Bethel CT) Margulies Marcel (Scarsdale NY) Ragusa R, Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control.
Hudson Derek (San Anselmo CA) Johnson Charles R. (Berkeley CA) Ross Michael J. (Hillsborough CA) Shoemaker Kevin R. (San Francisco CA) Cass Robert T. (San Mateo CA) Giebel Lutz B. (Burlingame CA), Thin film HPMP matrix systems and methods for constructing and displaying ligands.
Atwood John Girdner ; Mossa Albert Carmelo ; Goven Lisa May ; Williams Fenton ; Woudenberg Timothy M. ; Margulies Marcel ; Ragusa Robert P. ; Leath Richard ; Miles Clive, Two piece holder for PCR sample tubes.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.