IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
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출원번호 |
US-0666214
(2017-08-01)
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등록번호 |
US-10252262
(2019-04-09)
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발명자
/ 주소 |
- Colin, Bruno
- O'Bear, Raymond
- Paris, Cecile
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 |
피인용 횟수 :
0 인용 특허 :
37 |
초록
▼
The present invention is directed to sample test cards having an increased sample well capacity for analyzing biological or other test samples. In one embodiment, the sample test cards of the present invention comprise one or more fluid over-flow reservoirs, wherein the over-flow reservoirs are oper
The present invention is directed to sample test cards having an increased sample well capacity for analyzing biological or other test samples. In one embodiment, the sample test cards of the present invention comprise one or more fluid over-flow reservoirs, wherein the over-flow reservoirs are operatively connected to a distribution channel by a fluid over-flow channel. In another embodiment, the sample test cards may comprise a plurality of flow reservoirs operable to trap air thereby reducing and/or preventing well-to-well contamination. The test card of this invention may comprise from 80 to 140 individual sample wells, for example, in a test card sample test cards of the present invention have a generally rectangular shape sample test card having dimensions of from about 90 to about 95 mm in width, from about 55 to about 60 in height and from about 4 to about 5 mm in thickness.
대표청구항
▼
1. A method for filling a test sample card with a test sample, the method comprising the following steps of: a) providing a test sample containing or suspected of containing a microorganism;b) providing a sample test card, the sample test card comprising: a card body defining a first surface and a s
1. A method for filling a test sample card with a test sample, the method comprising the following steps of: a) providing a test sample containing or suspected of containing a microorganism;b) providing a sample test card, the sample test card comprising: a card body defining a first surface and a second surface opposite the first surface, a fluid intake port and a plurality of sample wells disposed between the first and second surfaces;a fluid channel network connecting the fluid intake port to the sample wells, the fluid channel network comprising at least one distribution channels, a plurality of fill channels operatively connecting the at least one distribution channel to the sample wells; andwherein the test card further comprises one or more fluid over-flow reservoirs, the over-flow reservoirs being operatively connected to the distribution channel by a fluid over-flow channel;c) filling or loading the test sample into the sample test card via the fluid intake port;wherein the plurality of sample wells are substantially filled with the test sample; andd) subsequently directing air or a non-aqueous liquid into the fluid channel network to reduce well-to-well contamination. 2. The method of claim 1, wherein the total volume of the test sample loaded is more than the aggregate total volume of the sample wells, and less than the total aggregate volume of the sample wells, the fluid channel network and the one or more fluid over-flow reservoirs. 3. The method of claim 1, wherein the total volume of the test sample is sufficient to fill the sample wells. 4. The method of claim 3, wherein the total volume of the test sample is sufficient to fill the fluid network and the sample wells. 5. The method of claim 4, wherein directing the air or the non-aqueous liquid into the flow channel network comprises displacing a portion of the test sample from the fluid network to the one or more fluid over-flow reservoirs. 6. The method of claim 1, wherein directing the air or the non-aqueous liquid into the flow channel network comprises displacing a portion of the test sample from the fluid network to the one or more fluid over-flow reservoirs. 7. The method of claim 1, wherein an over-flow channel connecting the distribution channel to the one or more fluid over-flow reservoirs has a reduced cross-section compared to the distribution channel, such that each of the sample wells is configured to fill with the test sample before the one or more over-flow reservoirs. 8. The method of claim 1, wherein the total volume of air aspirated into the sample test card is sufficient to fill the fluid channel network with air. 9. The method of claim 1, wherein the aspiration of air into the sample test card fills the fluid channel network with air and/or allows any excess fluid to flow into, or be captured by, the one or more fluid over-flow reservoirs. 10. The method of claim 1, wherein the test sample loaded onto the sample test card is from about 2 mL to about 3 mL. 11. The method of claim 1, wherein the test sample loaded onto the sample test card is from about 2.25 mL to about 2.75 mL. 12. The method of claim 1, wherein providing the sample test card further comprises depositing one or more reagents in the sample wells. 13. The method of claim 1, further comprising draining at least a portion of the test sample from the fluid channel network after the filling or loading the test sample into the sample test card. 14. The method of claim 1, wherein directing the air or the non-aqueous liquid into the fluid channel network comprises directing the non-aqueous liquid into the fluid channel network. 15. The method of claim 14, wherein the non-aqueous liquid is added to the test sample before the filling or loading the test sample into the sample test card. 16. The method of claim 1, wherein directing the air or the non-aqueous liquid into the fluid channel network comprises directing the air into the fluid channel network. 17. The method of claim 1, wherein the air or the non-aqueous liquid do not include the microorganism. 18. The method of claim 1, wherein the one or more fluid over-flow reservoirs further comprise an adsorbent for adsorbing excess liquid from the fluid channel network. 19. The method of claim 18, wherein the first and second surfaces sealed with a sealant tape covering the plurality of sample wells, and wherein the adsorbent is configured to bulge the sealant tape outwards to increase the volume of the adsorbent. 20. A sample test card comprising: a card body defining a first surface and a second surface opposite the first surface, a fluid intake port and a plurality of sample wells disposed between the first and second surfaces;a fluid channel network connecting the fluid intake port to the sample wells, the fluid channel network comprising at least one distribution channels, a plurality of fill channels operatively connecting the at least one distribution channel to the sample wells;wherein the test card further comprises one or more fluid over-flow reservoirs, the over-flow reservoirs being operatively connected to the distribution channel by a fluid over-flow channel;wherein the sample test card is configured to receive a test sample via the fluid intake port, such that the plurality of sample wells are substantially filled with the test sample;wherein the sample test card is subsequently configured to receive air or a non-aqueous liquid in the fluid channel network. 21. The sample test card of claim 20, wherein the one or more fluid over-flow reservoirs are configured to receive a portion of the test sample from the fluid channel network when the air or the non-aqueous liquid is received by the sample test card. 22. The sample test card of claim 20, wherein each of the sample wells defines a volume that is less than a volume of the one or more fluid over-flow reservoirs. 23. The sample test card of claim 20, wherein the sample wells further include a reagent deposited therein. 24. The test card of claim 20, further comprising a bubble trap in fluid communication with each of the sample wells, the traps being positioned at least partly above the respective wells. 25. The test card of claim 24, wherein the bubble traps include an opening on the first surface of the sample test card and do not extend to the second surface of the sample test card. 26. The test card of claim 24, wherein the one or more fluid over-flow reservoirs do not include the bubble trap.
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