Electromechanical lysis of bacterial pathogens using ion concentration polarization
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-013/00
C12N-001/06
출원번호
15728860
(2017-10-10)
등록번호
10640744
(2020-05-05)
발명자
/ 주소
Kim, Minseok
Han, Jongyoon
출원인 / 주소
Massachusetts Institute of Technology
대리인 / 주소
Elmore Patent Law Group, P.C.
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초록▼
Scalable, high throughput and power-efficient electromechanical lysis using low electric potential, which can be used for harvesting valuable intracellular biomolecules (DNA, RNA, and proteins) and metabolites (e.g., biodiesels, bioplastics, antibiotics, and antibodies), and for sterilizing large vo
Scalable, high throughput and power-efficient electromechanical lysis using low electric potential, which can be used for harvesting valuable intracellular biomolecules (DNA, RNA, and proteins) and metabolites (e.g., biodiesels, bioplastics, antibiotics, and antibodies), and for sterilizing large volume solutions (e.g. disinfection of bacterial contaminated drinking water). The method can be directly integrated with other microfluidic devices for all-in-one, fully integrated total-analysis systems for various bacterial (and cellular) studies and clinical applications.
대표청구항▼
1. A method of lysing a cell membrane comprising the steps: a. directing a fluid stream containing cells in a channel comprising an inlet and an outlet and defined, at least in part, by at least a first ion exchange membrane and at least a second ion exchange membrane, wherein the ion exchange membr
1. A method of lysing a cell membrane comprising the steps: a. directing a fluid stream containing cells in a channel comprising an inlet and an outlet and defined, at least in part, by at least a first ion exchange membrane and at least a second ion exchange membrane, wherein the ion exchange membranes are juxtaposed and characterized by the same charge;b. applying an electric field across the channel at a voltage and duration sufficient to cause helical electroconvective vortex formation across the channel, thereby lysing the cell membranes of the cells;c. collecting an output fluid stream comprising lysate from the outlet; andd. isolating the lysate from the output fluid stream. 2. The method of claim 1, wherein at least a first and at least a second juxtaposed ion exchange membrane are cationic exchange membranes. 3. The method of claim 1, wherein at least a first and at least a second juxtaposed ion exchange membrane are anionic exchange membranes. 4. The method of claim 1, wherein the channel is a microchannel. 5. The method of claim 1, further comprising the step of concentrating the lysate. 6. The method of claim 5, wherein at least one nonionic porous membrane is located at the outlet of the channel. 7. The method of claim 1, wherein the electric field is created by an electrode and a ground each located external and parallel to the channel. 8. The method of claim 7, wherein the electrode forms a second channel with at least a first ion exchange membrane and the ground forms a third channel with the at least a second ion exchange membranes. 9. The method of claim 8, wherein the second and third channel are filled with an electrolyte solution. 10. The method of claim 1, comprising a plurality or channels in parallel, each defined by an ion exchange membrane. 11. The method of claim 1, wherein the cell membrane is a bacterial cell membrane. 12. The method of claim 11, wherein isolating the lysate comprises isolating bacterial proteins, bacterial nucleic acids, or a combination thereof. 13. The method of claim 1, wherein isolating the lysate comprises isolating intracellular biomolecules. 14. The method of claim 13, wherein the intracellular biomolecules are selected from the group consisting of DNA, RNA, proteins, and bacterial metabolites, or a combination thereof. 15. The method of claim 14, wherein the bacterial metabolites comprise biodiesels, bioplastics, antibiotics, and antibodies.
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