Coaxial needle for fabricating a multi scale, multi layer blood vessel or vascular network employing 3D bioprinting
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61L-027/38
A61L-027/50
A61L-027/52
A61F-002/06
A61L-027/16
A61L-027/18
B29C-064/209
B33Y-010/00
B33Y-030/00
B29C-064/118
A61L-027/22
B29C-064/135
B33Y-070/00
출원번호
15864387
(2018-01-08)
등록번호
10857260
(2020-12-08)
발명자
/ 주소
Cui, Haitao
Zhu, Wei
Zhang, Lijie Grace
출원인 / 주소
The George Washington University
대리인 / 주소
Christensen, Kory D.
인용정보
피인용 횟수 :
0인용 특허 :
0
초록▼
A coaxial needle having two or more passageways for forming a biomimetic bioprinted blood vessel tubular construct. The coaxial needle has an external needle passageway and an internal needle passageway separated by an internal barrier, and having a nozzle at an end thereof. A bioink is provided flo
A coaxial needle having two or more passageways for forming a biomimetic bioprinted blood vessel tubular construct. The coaxial needle has an external needle passageway and an internal needle passageway separated by an internal barrier, and having a nozzle at an end thereof. A bioink is provided flowable through the external needle passageway and a crosslinking solution is provided flowable through the internal needle passageway. The crosslinking solution contacts the bioink as the crosslinking solution and bioink exit the nozzle thereby forming a tubular blood vessel construct.
대표청구항▼
1. A method of forming a biomimetic blood vessel comprising: flowing a bioink through an external needle passageway of a coaxial needle and out through a nozzle of the coaxial needle, the nozzle being at one end of the coaxial needle;flowing a crosslinking solution through an internal needle passage
1. A method of forming a biomimetic blood vessel comprising: flowing a bioink through an external needle passageway of a coaxial needle and out through a nozzle of the coaxial needle, the nozzle being at one end of the coaxial needle;flowing a crosslinking solution through an internal needle passageway of the coaxial needle and out through the nozzle of the coaxial needle, the external needle passageway and internal needle passageway being separated by a barrier; andcontacting the crosslinking solution with the bioink as the crosslinking solution and the bioink exit the nozzle thereby forming a tubular blood vessel construct,wherein the bioink comprises a gelatin methacrylate functionalized with a catechol. 2. The method of claim 1, wherein the bioink is cell-laden. 3. The method of claim 2, wherein the bioink is cell-laden with smooth muscle cells. 4. The method of claim 1, wherein the crosslinking solution comprises a crosslinking agent. 5. The method of claim 4, wherein the crosslinking agent is an oxidant. 6. The method of claim 5, wherein the oxidant is a periodate salt or a derivative thereof. 7. The method of claim 1, wherein the crosslinking solution comprises a removable fugitive ink. 8. The method of claim 7, wherein the fugitive ink comprises a block copolymer of ethylene oxide and propylene oxide. 9. The method of claim 1, wherein the crosslinking solution is cell-laden. 10. The method of claim 1, wherein the crosslinking solution is cell-laden with endothelial cells. 11. The method of claim 1, further comprising flowing a cell-laden fluid in a passageway between the external needle passageway and the internal needle passageway. 12. A system comprising: a coaxial needle having an external needle passageway and an internal needle passageway separated by an internal barrier, and having a nozzle at an end thereof;a bioink flowable through the external needle passageway; anda crosslinking solution flowable through the internal needle passageway,the crosslinking solution contacting the bioink as the crosslinking solution and bioink exit the nozzle thereby forming a tubular blood vessel construct,wherein the bioink comprises a gelatin methacrylate functionalized with a crosslinkable group, andthe crosslinking solution comprises a crosslinking agent, wherein the crosslinking agent is a periodate salt or a derivative thereof. 13. The system of claim 12, wherein the crosslinking solution comprises a removable fugitive ink. 14. The system of claim 13, wherein the fugitive ink comprises a block copolymer of ethylene oxide and propylene oxide. 15. The system of claim 12, wherein the bioink is cell-laden with smooth muscle cells, andthe crosslinking solution is cell-laden with endothelial cells,wherein the tubular blood vessel construct comprises a bilayer of cells having an outer layer containing smooth muscle cells and an inner layer containing endothelial cells. 16. The system of claim 12, further comprising the tubular blood vessel construct being embedded in a scaffold. 17. A method of forming a biomimetic blood vessel comprising: flowing a bioink through an external needle passageway of a coaxial needle and out through a nozzle of the coaxial needle, the nozzle being at one end of the coaxial needle;flowing a crosslinking solution through an internal needle passageway of the coaxial needle and out through the nozzle of the coaxial needle, the external needle passageway and internal needle passageway being separated by a barrier;flowing a cell-laden fluid in a passageway between the external needle passageway and the internal needle passageway; andcontacting the crosslinking solution with the bioink as the crosslinking solution and the bioink exit the nozzle thereby forming a tubular blood vessel construct. 18. The method of claim 17, wherein the crosslinking solution comprises an oxidant and a removable fugitive ink, wherein the oxidant is a periodate salt or a derivative thereof, and the removable fugitive ink comprises a block copolymer of ethylene oxide and propylene oxide. 19. The method of claim 17, wherein the bioink is cell-laden with smooth muscle cells. 20. The method of claim 17, wherein the crosslinking solution is cell-laden with endothelial cells. 21. A system comprising: a coaxial needle having an external needle passageway and an internal needle passageway separated by an internal barrier, and having a nozzle at an end thereof;a bioink flowable through the external needle passageway, wherein the bioink comprises a gelatin methacrylate functionalized with a catechol; anda crosslinking solution flowable through the internal needle passageway, wherein the crosslinking solution comprises an oxidant or a derivative thereof,the crosslinking solution contacting the bioink as the crosslinking solution and bioink exit the nozzle thereby forming a tubular blood vessel construct. 22. The system of claim 21, wherein the crosslinking solution comprises a removable fugitive ink, the fugitive ink comprising a block copolymer of ethylene oxide and propylene oxide. 23. The system of claim 22, wherein the crosslinking solution comprises endothelial cells.
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