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Provided are a PD-1 CAR-T cell, a preparation method therefor, and an application thereof. By means of chimeric antigen receptor-modified T cell transformation, PD-1-CD8TM-4-1BB-CD3�� molecules are expressed in a T cell. The CAR T cell prepared by the method can specifically recognize and bind with

Provided are a PD-1 CAR-T cell, a preparation method therefor, and an application thereof. By means of chimeric antigen receptor-modified T cell transformation, PD-1-CD8TM-4-1BB-CD3�� molecules are expressed in a T cell. The CAR T cell prepared by the method can specifically recognize and bind with tumor cells with high PDL-1 protein expression, and is applicable to preparation of a drug for preventing and treating tumor diseases.

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