초록 ▼

1차년도에는 정상인과 요농축능 장애 환자에서 탈수 및 항이뇨 호르몬 투여 전후에 요량, 요 전해질 배설률, 요 삼투질 배설률, 신세뇨관 수분 재흡수율, 요수분 재흡수율 둥의 신기능 지표를 비교하여 기존 표준 평가법의 문제점을 검토하였다. 2차년도의 연구에서는 웅성 Sprague-Dawley 백서를 대상으로 기저상태, 탈수상태 및 항이뇨 호르몬인 vasopressin, oxytocin 투여 후 요지표를 측정하고 각 실험군에서 AQP2 항체를 사용하여 immunoblot과 신조직내에서의 면역조직 화학법을 시행하였다. 이를 통하여 동물모

1차년도에는 정상인과 요농축능 장애 환자에서 탈수 및 항이뇨 호르몬 투여 전후에 요량, 요 전해질 배설률, 요 삼투질 배설률, 신세뇨관 수분 재흡수율, 요수분 재흡수율 둥의 신기능 지표를 비교하여 기존 표준 평가법의 문제점을 검토하였다. 2차년도의 연구에서는 웅성 Sprague-Dawley 백서를 대상으로 기저상태, 탈수상태 및 항이뇨 호르몬인 vasopressin, oxytocin 투여 후 요지표를 측정하고 각 실험군에서 AQP2 항체를 사용하여 immunoblot과 신조직내에서의 면역조직 화학법을 시행하였다. 이를 통하여 동물모텔의 결파롤 인체에 적용할 기법을 도출하였다. 3차년도에서는 요 AQP 측정법을 정립하여 청상인과 요농축능 장애 환자에서 질병에 따른 요 AQP을 측정하였고, 수분배설에 장애가 있는 신증후군 환자의 신조직내에서 AQP의 발현양상을 관찰하였다. 연구결과 요 AQP치를 이용한 새로운 기능 평가법의 기반 기술을 확보하였다.

Abstract ▼

In the first year, we investigated whether exogenous antidiuretic hormones or water restriction affected urinary concentration in human. The study was performed in 10 healthy male volunteers, 7 patients with central diabetes insipidus (CDI) and 3 patients with nephrogenic diabetes insipidus (NDI). T

In the first year, we investigated whether exogenous antidiuretic hormones or water restriction affected urinary concentration in human. The study was performed in 10 healthy male volunteers, 7 patients with central diabetes insipidus (CDI) and 3 patients with nephrogenic diabetes insipidus (NDI). Two-hour urine was collected before and after the administration of antidiuretic hormone. Urine volume and free-water clearance decreased significantly with the adminstration of oxytocin and dDA VP in normal volunteers and CDI patients but not in NDI patients. Urine osmolality increased with the administration of oxytocin and dDA VP in normal volunteers and CDI patients but not in NDI patients. These results indicate that exogenous oxytocin has an antidiuretic effect in human.
In the second year, to examine whether oxytocin is involved in the regulation of AQP2 in renal collecting ducts as an antidiuretic honnone, the changes in the distribution and the expression of AQP2 in the rat kidney after adminstration of oxytocin was observed by immunohistochemistry and immunoblot Male Sprague-Dawley rats were divided into four groups as control, oxytocin, vasopressin and water restriction group(48-hour water restriction). Urine volume was significantly decreased after intraperitoneal injection of 5U vasopressin and after 48-hour water restriction compared with controls. After intraperi~oneal injection of IOU oxytocin, there was no significant change in urine volume. Urine osmolality was significantly increased in oxytocin group, vasopressin group and water restriction group compared with controls. The most striking changes in the distribution of AQP2 were observed in the IMCD. In the control rats, IMCD cells exhibited predominant diffuse cytoplasmic lebeling of both apical and basolateral membrane. In oxytocin group. IMCD cells exhibited distinct labeling of both basolateral and apical membrane. In vasopressin and water restriction group, labeling of IMCD cells was confined to apical membrane. AQP2 was also redistributed from basolateral membrane and cytoplasm to apical membrane in connecting tubules in vasopressin and water restriction group. Oxytocin and 48-hour dehydration induced about two-fold increase in the amount of AQP2 protein comparing with that of control group. In summary, oxytocin induced the redistribution of AQP2 from cytoplasm to both apical and basolateral membrane and the upregulation of AQP2 protein expression in rat kidney inner medulla. These findings suggest that oxytocin acts as an antidiuretic hormone by regulation of AQP2 in kidney collecting duct.

목차 Contents

• 표지...1
• 제출문...2
• 요약문...3
• SUMMARY...6
• CONTENTS...7
• 목차...8
• 제1장. 서론...9
• 제1절. 연구개발의 목적...9
• 제2절. 연구개발의 연차별 목표...9
• 제3절. 연구개발의 필요성 및 범위...9
• 제2장. 국내외 기술개발 현황...13
• 제3장. 연구개발수행 내용 및 결과...15
• 제1절. 1차년도...15
• 1. 연구수행 내용...15
• 2. 연구방법...15
• 3. 연구결과...16
• 제2절. 2차년도...20
• 1. 연구수행 내용...20
• 2. 연구방법...20
• 3. 연구결과...21
• 제3절. 3차년도...30
• 1. 연구수행 내용...30
• 2. 연구방법...30
• 3. 연구결과...31
• 제4장. 연구개발목표 달성도 및 대외기여도...38
• 제1절. 1차년도...38
• 제2절. 2차년도...39
• 제3절. 3차년도...40
• 제5장. 연구개발결과의 활용성과 및 계획...42
• 1. 계량적 성과...42
• 2. 성과내용 기술...42
• 3. 활용계획...43
• 제6장. 참고문헌...45