초록

본연구에서는 해양 발광세균에서의 생체 발광 반응의 기작을 규명하기위하여 새로운 형광성 기질을 합성하고 이를 이용하여 기질 저해 현상의 원인과 발광시 에너지 전달을 규명하고자 하였다. 또 응용면에서는 이러한 생체 발광반응을 이용하여 생체내 생리 대사에 매우 중요한 효소들의 활성 측정법을 개발하였다.

Abstract ▼

In this research, in order to elucidate the reaction mechanism of bacterial bioluminescence, a new fluorescent substrate was synthesized and energy transfer and aldehyde substrate inhibition were studied.
For an application of bioluminescence reaction, an assay method for phospholipase A2 which

In this research, in order to elucidate the reaction mechanism of bacterial bioluminescence, a new fluorescent substrate was synthesized and energy transfer and aldehyde substrate inhibition were studied.
For an application of bioluminescence reaction, an assay method for phospholipase A2 which has a critical role in biometabolism was developed.

목차 Contents

• 해양 세균의 생체 발광 반응 기작 ...9
• Bloluminescent Assay of Phospholipase A2 Using Luminescent Marine Bacterium vibrio harveyi mutant m-17 ...14
• Mechanism of Aldehyde Inhibition of Vibrio harveyi Luciferase ...18
• Bacterial luciferese 반응 기작 규명을 위한 형광성 기질의 합성 ...25