초록

$\bullet$ 마이크로시스틴 단일 클론 항체를 이용한 형광 면역 chromatograph rapid kit의 개발
$\bullet$ 마이크로시스틴 정량에 필요한 고효율 휴대용 형광 reader의 개발
$\bullet$ 마이크로시스틴 원격 검침 시스템의 개발

Abstract ▼

Recently, the researches on freshwater cyanobacteria' toxin have been paid a great attention. Not only have toxin micorcystins (MCs) produced by blue-green algae become a great threat to the welfare of human and animal but also are in danger to worldwide natural resources because MCs are capable of

Recently, the researches on freshwater cyanobacteria' toxin have been paid a great attention. Not only have toxin micorcystins (MCs) produced by blue-green algae become a great threat to the welfare of human and animal but also are in danger to worldwide natural resources because MCs are capable of blooming by eutrophication of water ecosystem and contaminating the drinking water. In this study, we tried to develop a biosensor for Mcs to take a pre-action against toxins of Mcs, and apply it to system of water administration. The developed MCs' sensor, if it is installed at the process of water quality control, could play an important role in evaluating water system.
We produced mouse monoclonal antibody against MCs. The immunization, cell fusion, andscreening hybridoma cells were conducted according to a standard method. The MCs from cyanobacteria were purified by supercritical fluid extraction method, which is an assay method developed by our group. We report rapid quantification methods of MCs using fluorescence for a detection signal and a lateral-flow-type immuno-chromatography as a separation system. The developed biosensor systemshowed a high specificity for immune assay, a rapidity to complete test within 10 min, and a high sensitivity associated with fluorescence reader. The fluorescence immune assay system for quantification of MCs, which fluorescence bead is incorporated instead of Alexa 647, has a linear dynamic range of 50-1600 pg/ml and a stability for 24 month at room temperature.
Since the developed assay method through this study is applicable to other contaminants such as dioxin, PCBs and so on, it will give a great impact on water/air pollution-related fields.

목차 Contents

• 제 1 장 서론...9
• 제 1 절 연구개발의 필요성...9
• 제 2 장 국내외 기술개발 현황...20
• 제 1 절 마이크로시스틴 바이오센서의 개발...20
• 제 3 장 연구개발 수행 내용 및 결과...25
• 제 1-1 절 마이크로시스틴 LR, RR, 등을 많이 함유한 새로운 종의 검색 및 실험실...25
• 제 1-2 절 새로운 남조류 종으로부터 마이크로시스틴 추출...28
• 제 1-3 절 마이크로시스틴 생산 및 다량 정제법 개발...34
• 제 1-4 절 마이크로시스틴 센서의 마이크로시스틴 RR과 YR에 대한 Cross Reactivity Test...46
• 제 1-5 절 마이크로시스틴 센서의 Linear dynamic range Test...49
• 제 1-6 절 마이크로시스틴 센서의 Sensitivity 와 Stablility Test...51
• 제 2-1 절 마이크로시스틴 항체의 대량 생산...54
• 제 2-2 절 마이크로시스틴 검출 형광 스트립 개발...61
• 제 3-1 절 검지 장치의 개발...102
• 제 4 장 연구개발목표 달성도 및 대외기여도...139
• 제 1 절 연구개발목표 달성도...139
• 제 2 절 대외기여도...140
• 제 5 장 연구개발결과의 활용계획...142
• 제 1 절 연구개발사업성과의 사업화 가능성...142
• 제 2 절 기술 개발에 따라 예상되는 파급효과 및 활용방안...142
• 제 6 장 참고문헌...146