초록 ▼

본 과제에서는 근육줄기세포/생리활성물질 함유 나노캡슐이 균일하게 혼합되어 있고, 체외에서 용액상태로 손쉽게 주사주입이 가능하며, 체내에서는 이들이 안정하게 유지될 수 있는 겔 상태로 바뀌는 새로운 개념의 주사주입형 요실금 치료제제를 제조하고자 하였음. 변형된 전 플라스크법으로 분리, 동정된 근육줄기세포의 근육세포로의 분화 (in vitro & in vivo), 요실금 치료효과 (in vivo) 및 세포치료제 (gene transfection)로의 응용가능성을 확인할 수 있었으며, 근육줄기세포의 분화 조절 및 세포 성장에 영향

본 과제에서는 근육줄기세포/생리활성물질 함유 나노캡슐이 균일하게 혼합되어 있고, 체외에서 용액상태로 손쉽게 주사주입이 가능하며, 체내에서는 이들이 안정하게 유지될 수 있는 겔 상태로 바뀌는 새로운 개념의 주사주입형 요실금 치료제제를 제조하고자 하였음. 변형된 전 플라스크법으로 분리, 동정된 근육줄기세포의 근육세포로의 분화 (in vitro & in vivo), 요실금 치료효과 (in vivo) 및 세포치료제 (gene transfection)로의 응용가능성을 확인할 수 있었으며, 근육줄기세포의 분화 조절 및 세포 성장에 영향을 미치는 생리활성물질의 서방형 방출이 가능한 나노캡슐, 그리고 이들이 혼합되어 주사주입이 용이하고 체내 안정성을 지녀 요실금 치료효과가 우수한 주사제제를 개발할 수 있었음. 또한 단순 요도 지지체가 아닌 요도 주변 근육조직의 기능까지 향상시킬 수 있는, 근육줄기세포, 생리활성물질이 도입된 나노섬유 부직포로 구성된 요실금 수술 치료용 sling 재료를 개발할 수 있었음.

Abstract ▼

1. Background and Purpose
- Urinary incontinence, which is defined as the involuntary loss of urine, causes serious medical and social problems, having a deep influence of a patient`s psychological state.
- These disease is a common, distressing condition that affects at least 14% of women age

1. Background and Purpose
- Urinary incontinence, which is defined as the involuntary loss of urine, causes serious medical and social problems, having a deep influence of a patient`s psychological state.
- These disease is a common, distressing condition that affects at least 14% of women aged 30 years or older.
- In this study, we purposed to develop muscle stem cell (MSC)/biocompatible polymer hybrid materials (gel or solid type) for the effective treatment of urinary incontinence.
II. Methods
The muscle stem cells (MSCs) were isolated from the gastrocnemius muscles of Sprague-Dawley rats and humans by a modified preplate technique and characterized them through FACS and immunostaining (CD34, CD4S and desmin)
- The growth factors-loaded PLGA nanospheres were prepared to provide sustained release of them.
- The therapeutic efficiency for urinary incontinence of MSCs/biocompatible polymer hybrid materials and contractable (bioactive) scaffold was investigated by in vivo animal study using rat model.
III. Results
- More primitive stem cells were obtained by a modified preplate technique, compared to a conventional method
- It was observed that the MSCs are differentiated to smooth muscle celsl and skeletal muscle cells by co-culture method and VEGF addition in cell culture medium (in vitro). The MSCs were also differentiated to muscle cells by interaction with surrounding tissues (in vivo).
- The transfection of EGFP and VEGF into the MSCs was also observed, which can be applied cell therapy
- The growth factors were continuously released from the growth factors-loaded nanospheres with time and their activity was similar to the original stuff
- It was observed that the MSCs/polymer hybrid materials (gel type) had good injectability, volume retention and biocompatibility, inducing effective cure of the urinary incontinence as a bulking agent (in vivo)
- It was observed that the MSCs/polymer hybrid materials (nanofiber nonwoven sheet) showed also effective cure of the urinary incontinence due to synergistic effect of the nanofibrous nonwoven sheet (physical support for urethral) and MSCs (differentiation to muscle)
IV. Applications
- The MSCs/polymer hybrid materials can be applied to various diseases such as fecal incontinence, esophageal reflux, cardiac insufficiency etc.
- The MSCs/polymer hybrid materials can be also used for the tissues regeneration including bladder, skin, bone cartilage and nerve etc.
- The growth factor loading technique onto the nanoshpere can be applied for delivery system of protein drugs and anti-cancer drugs

목차 Contents

• 제 1 장 연구개발과제의 개요...40
• 제 2 장 국내외 기술개발 현황...45
• 제 3 장 연구개발수행 내용 및 결과...47
• 제 4 장 목표달성도 및 관련분야에의 기여도...81
• 제 5 장 연구개발결과의 활용계획...83
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보...84
• 제 7 장 참고문헌...87
• 제 8 장 첨부서류...89