초록 ▼

발암유전자 (E6, E7)와 항암유전자 (p53, Rb) 간의 결합 반응에 의한 ELISA 방법을 이용하여 발암유전자 (E6, E7)억제인자의 탐색 및 확보 Database화 함.
유효 성분 중 항암 및 항염증 효능이 뛰어난 Rosmarinic acid 유도체, 플라본 (Tetramethoxy flavone, jaceosidin) 등의 유도체의 개량 및 선별-항암 및 항염증 효능이 뛰어난 물질의 스크리닝 방법 개량 및 물질선별
추출 및 합성 유효성분들 중에서 항염증 및 항암활성이 뛰어난 성분의 선별 및 각 성분들의 효능

발암유전자 (E6, E7)와 항암유전자 (p53, Rb) 간의 결합 반응에 의한 ELISA 방법을 이용하여 발암유전자 (E6, E7)억제인자의 탐색 및 확보 Database화 함.
유효 성분 중 항암 및 항염증 효능이 뛰어난 Rosmarinic acid 유도체, 플라본 (Tetramethoxy flavone, jaceosidin) 등의 유도체의 개량 및 선별-항암 및 항염증 효능이 뛰어난 물질의 스크리닝 방법 개량 및 물질선별
추출 및 합성 유효성분들 중에서 항염증 및 항암활성이 뛰어난 성분의 선별 및 각 성분들의 효능 검정
발암억제 효능물질의 ELISA 검색시스템 및 암 및 염증질환억제 시스템 확립 및 활용
자궁경부암 바이러스 기능 억제효능 자생식물체 추출물 20 가지 이상 확보.
염증 및 항 종양제 스크리닝 시스템 개발 및 시스템의 검증완료-논문 발표
자생식물 200여 개로부터 20가지 이상 후보 추출물 확보
자생 식물 박하, 애엽, 인진, Pueraria 뿌리 등에서 분리된 Rosmarinic acid 유도체와 Caffeic acid 유도체, methoxyflavone, jaceosidin, caffeoyl quinic acld, spinasterol의 항산화, 항염증 및 항암 효과 입증-논문 발표
자궁경부암 억제물질 Tetramethoxy falvone, jaceosidin 등의 세포 독성 조사-효과 입증 논문 발표

Abstract ▼

Normal cells have special self-regulating activities; Positive regulators such as cyclins and cyclin-dependent kinases (cdk) which induce proliferation and growth, and negative regulatory components such as cdk inhibitors are tightly regulated to control homeostasis. Thus, many trials have been perf

Normal cells have special self-regulating activities; Positive regulators such as cyclins and cyclin-dependent kinases (cdk) which induce proliferation and growth, and negative regulatory components such as cdk inhibitors are tightly regulated to control homeostasis. Thus, many trials have been performing for the development of the diagnosis of cancer, therapeutics and screening system of anti-cancer agents. For the screening of anti-cancer agents target-oriented to inhibit the function and expression of cancer oncogenes E6, E7, this study is elucidated on the applications of screening systems to isolate new factors to inhibit the infection of virus and activity of oncogenes. Tetramethoxy flavones isolated from Artemesia and korean natural plants and their synthetic derivatives are being used to develope modulators for immune diseases of women infected by cervical carcinoma-inducing viruses. Over 50% of human cancers screened to date are caused by mutation of tumor suppressors p53 and Rb proteins. Other cancers have been described that p53, pRb have been inactivated by virus infection and other kinds of cell cycle modulators and tumor suppressors are inactivated or mutated by mutation or inactivation of proteins related to cell cycle. Therefore, based on interaction between oncogenes and tumor suppressors, proteins or genes related to cell cycle will be informed and much more progresses will be performed in the development of anti-cancer agents. Efficient various systems for screening modulators targeting to oncogenes from cancer cells and natural products have been elucidated based on interactions between oncogenes and tumor suppressors(ELISA based on interaction between oncogene E6 and E6AP; ELISA based on interaction between oncogene E6 and p53; ELISA based on interaction between oncogene E7 and Rb; ELISA based on interaction between oncogene E6AP and p53; Screening assay systems using transactivation of heterologous viral promoters of oncogenes E6, E7). Those systems can be applied to the screening of anti-oncogenes of HPV, or anti-inflammatory modulators and preventive and therapeutic trials to cervical cancers. Several screening systems to isolate new factors or materials to inhibit the function and activity of oncogenes have been used. In this study, we have produced, purified the oncogenes (E7, E6, E6AP) and tumor suppressor proteins(p53, Rb) by using gene recombination technology, and further established the in vitro binding screening system to test the function of these genes. These technologies were patented (277661, 329936, 380288). This study is focused as follows; By using in vitro systems based on the interactions between oncogenes and tumor suppressors (ELISA based on E6-p53 binding; ELISA based on E6AP-p53 binding; ELISA based on E6-E6AP binding; ELISA based on E7-Rb binding), cell cycle or apoptosis modulating factors can be identified and some of these factors can be used as putative anti cancer agent which can inhibit the growth of cancer cells or inhibit the inflammatory responses. These systems will be also used to screen the anti-cancer agents from the extracts of natural products such as oriental medicines and plants. By using these systems, several modulating factors such as tetramethoxy flavones are being isolated and characterized. Some of these factors are being derived from chemical synthesis and investigated how these factors are effective against infection and cervical cancers.

목차 Contents

• 표지...1
• 제출문...2
• 보고서 초록...3
• 요약문...4
• SUMMARY...12
• CONTENTS...14
• 목차...15
• 제1장 연구개발과제의 개요...16
• 제2장 국내외 기술개발 현황...20
• 제3장 연구개발수행 내용 및 결과...21
• 제4장 목표달성도 및 관련분야에의 기여도...33
• 제5장 연구개발결과의 활용계획...36
• 제6장 연구개발과정에서 수집한 해외과학기술정보...38
• 제7장 참고문헌...40