초록 ▼

$\blacktriangleright$ 세포분열 및 세포사멸 과정에서의 mammalian Polo-like kinase1 (Plk1) 표적단백질 동정 및 Plk1 표적 단백질간의 network 규명
$\blacktriangleright$ 유사분열기의 DNA damage checkpoint 과정 연구, 관련 인자들과 Plk1간의 상호 연관성 연구
$\blacktriangleright$ 종양 치료제 target 유전자로서의 Plk1의 기능 검증
Part 1. 세

$\blacktriangleright$ 세포분열 및 세포사멸 과정에서의 mammalian Polo-like kinase1 (Plk1) 표적단백질 동정 및 Plk1 표적 단백질간의 network 규명
$\blacktriangleright$ 유사분열기의 DNA damage checkpoint 과정 연구, 관련 인자들과 Plk1간의 상호 연관성 연구
$\blacktriangleright$ 종양 치료제 target 유전자로서의 Plk1의 기능 검증
Part 1. 세포 분열기 및 세포 사멸 과정에서 Plk1 target 단백질 검색 Plk1의 domain specific bait를 이용, yeast two hybrid및 면역 침강법에 의해 Plk1의 target 단백질들을 스크리닝 하였고, Plk1의 기질로써의 가능성을 타진. Plk1에 의해 인산화 되는지 검증.
$\circledcirc$ Young-Joo Jang 외 1 (2006) Screening of Domain-specific Target Proteins of Polo-like Kinase 1:Construction and Application of Centrosome/Kinetochore-specific Targeting Peptide. Journal of Biochemistry and Molecular Biology 39, 709-716.
Part 2. 유사분열기의 DNA damage checkpoint 과정 연구, 관련 인자들과 Plk1간의 상호 연관성 연구 유사분열기에만 특이적으로 작용하는 Plk1의 또 다른 조절기전의 발굴. 유사분열기 DNA damage에 반응하는 세포에서 Plk1의 탈인산화 최초 규명. $\circledcirc$ Young-Joo Jang 외 4 (2007) Regulation of Polo-like Kinase 1 by DNA Damage in Mitosis: inhibition of mitotic Plk-1 by protein phosphatase
2A. Journal of Biological chemistry 282, 2473-2482.
Part 3. 종양치료제 타겟 검증다양한 종양조직 및 암세포에서 Plk1의 발현 수준 조사하고 Plk1의 과다 발현 및 억제시 나타나는 세포학적 분자적 효과의 분석 연구
$\circledcirc$ Young-Joo Jang 외 2 (2006) Oncogenic effect of Polo-like kinase 1 expression in human gastric carcinomas. International Journal of
Oncology 29, 589-594
- 연구내용에서 주제별로 연구한 내용과 이로부터 얻은 연구성과를 나열하였음.
- 3년의 연구기간 동안 Plk1의 target protein 및 Plk1의 새로운 조절기전, 그리고 새로운 기능을 밝혀내었고, 특히 한국형 위암에서 Plk1이 암 표적 인자가 될 가능성을 밝혔음.
- SCI 급 논문 3편에 이미 게재완료 하였으며, 2005년-2007년에 걸쳐 국내 및 국제 학회에 관련된 결과물을 6편 발표하였고, 현재 Plk1의 표적단백질로써 세포사멸에 작용하거나 유사분열기 염색체 분열에 관련하는 인자들의 기능 연구결과를 논문 준비 중에 있음.

Abstract ▼

$\blacktriangleright$ Characterization of downstream target proteins of Polo-like kinase 1 (plk1)
in cell division cycle and apoptosis
$\blacktriangleright$ Studies on the novel regulatory mechanisms of Plk1 in mitotic DNA checkpoint
and apoptosis
$\blacktriang

$\blacktriangleright$ Characterization of downstream target proteins of Polo-like kinase 1 (plk1)
in cell division cycle and apoptosis
$\blacktriangleright$ Studies on the novel regulatory mechanisms of Plk1 in mitotic DNA checkpoint
and apoptosis
$\blacktriangleright$ Therapeutic application of Plk1 for pharmacological target of cell cycle
regulator and anti-tumor drug
Part 1. Characterization of downstream target proteins of Polo-like kinase 1 (plk1) in cell division cycle and apoptosis. By using domain specific baits of Plk1, Plk1-target proteins were screened and their functions and the possibility of a substrate of Plk1 were studied by yeast two hybrid method and immuno-protein biochemistry.
$\circledcirc$ Young-Joo Jang et al. (2006) Screening of Domain-specific Target Proteins of Polo-like Kinase 1:Construction and Application of Centrosome/Kinetochore-specific Targeting Peptide. Journal ofBiochemistry and Molecular Biology 39, 709-716.
Part 2. Studies on the novel regulatory mechanisms of Plk1 in mitotic DNA checkpoint and apoptosis
A novel regulatory mechanism of Plk1 in mitotic DNA checkpoint was investigated in human cancer cells. We first up found that Plk1 is dephosphoyrlated in vivo.
$\circledcirc$ Young-Joo Jang et al. (2007) Regulation of Polo-like Kinase 1 by DNA Damage in Mitosis: inhibition of mitotic Plk-1 by protein phosphatase 2A. Journal of Biological chemistry 282, 2473-2482. Part 3. Therapeutic application of Plk1 for anti-cancer target The relationship between cancer and Plk1 was investigated in Human gastric tumor tissue. Plk1 could be a cancer marker or useful anti-cancer drug target in this cancer.
$\circledcirc$ Young-Joo Jang et al. (2006) Oncogenic effect of Polo-like kinase 1 expression in human gastric carcinomas. International Journal of
Oncology 29, 589-594
During 3 years, we found that a novel regulation mechanism of Plk1 in mitosis and several target proteins of Plk1. And, we investigated that Plk1 could be a target protein or a gastric cancer marker by using several
hundreds of cancer tissues from Korean gastric cancer patients. We already had published 3 papers (SCI level) and 3 presentations about Plk1 regulation and cancer-related study as mentioned above, and are preparing for publishing of 2-3 papers more about apoptotic target proteins of Plk1 and a substrate in chromosome segregation.

목차 Contents

• Ⅰ. 연구계획 요약문...3
• 1. 국문요약문 ...4
• Ⅱ. 연구결과 요약문...4
• 1. 국문요약문 ...4
• 2. 영문요약문 ...5
• Ⅲ. 연구내용...6
• 1. 서론 ...6
• 2. 연구방법 및 이론 ...13
• 3. 결과 및 고찰 ...16
• 4. 결론 ...42
• 5. 인용문헌 ...43
• 6. 연구성과 ...48