보건의료기술진흥사업;국산 소나무껍질추출물을 이용한 주름개선용 패취 개발 Health&Medical Technology R&D Program;Development of a wrinkle care patch using the Korean pine bark extract원문보기
보고서 정보
주관연구기관
뉴트라팜(주)
연구책임자
한완택
참여연구자
김대덕
보고서유형
최종보고서
발행국가
대한민국
언어
한국어
발행년월
2007-05
과제시작연도
2006
주관부처
보건복지부
사업 관리 기관
한국보건산업진흥원 Korea Health Industry Development Institute
국산 소나무껍질로부터 강력한 항산화 물질(조추출물)을 5.7Kg 추출하였고, 알콜용액에 대한 용해도 차이에 의하여 조추출물의 분획물을 제조한 후 분획물의 항산화활성을 측정한 결과 기존 제품에 비하여 1.5배의 활성이 증가되었다. 분획물의 수용액에 대한 강제환경시험을 통하여 상온에서 비교적 안정성이 높은 물질로 확인되었으며, 분획물에 대한 HPLC분석결과 수용성분획물과 난용성분획물의 Profile이 상이함을 알수 있었다. 분획물을 이용한 패치 제제화시험를 성공적으로 실시하였고, 분획물의 수용액과 겔 상태에서 실험동물의 피부투과도와 피
국산 소나무껍질로부터 강력한 항산화 물질(조추출물)을 5.7Kg 추출하였고, 알콜용액에 대한 용해도 차이에 의하여 조추출물의 분획물을 제조한 후 분획물의 항산화활성을 측정한 결과 기존 제품에 비하여 1.5배의 활성이 증가되었다. 분획물의 수용액에 대한 강제환경시험을 통하여 상온에서 비교적 안정성이 높은 물질로 확인되었으며, 분획물에 대한 HPLC분석결과 수용성분획물과 난용성분획물의 Profile이 상이함을 알수 있었다. 분획물을 이용한 패치 제제화시험를 성공적으로 실시하였고, 분획물의 수용액과 겔 상태에서 실험동물의 피부투과도와 피부누적량을 측정한 결과 분획물이 실제로 피부를 통과하고 또한 피부에 누적됨을 밝혔다. 따라서 소나무껍질추출물의 분획물을 함유한 제제를 통하여 패치제 및 겔 제제의 개발이 가능함을 확인하였고, 외부 전문기관에 의뢰하여 분획물을 함유한 패치 시제품을 제작하였다.
Abstract▼
Pine bark extract is well-known functional material as very powerful antioxidant, anti-inflammatory, antibiotic, etc. French maritime pine bark extract (brand name: Pycnogenol) of Horphag Research has over 200 scientific research papers, which helps Pycnogenol for monopolizing the world market over
Pine bark extract is well-known functional material as very powerful antioxidant, anti-inflammatory, antibiotic, etc. French maritime pine bark extract (brand name: Pycnogenol) of Horphag Research has over 200 scientific research papers, which helps Pycnogenol for monopolizing the world market over 30 years and Korean red pine bark extract was first manufactured by the patent technology of Nutrapharm in Korea in 2006. However, there are few research papers on Korean red pine bark extract that cause serious barrier for marketing Korean product. Therefore, this research has been focused on developing the bark extract fraction suitable for patch application, stability study of the fraction, and skin permeation study of the patch with the fraction which aimed for developing very competitive core material for cosmetic application, internationally. Crude pine bark extract, a total of 5.7Kg, was obtained from grinding, extracting in ethanol solution, concentrating, drying and pulverizing of Korean red pine bark, and extraction conditions were established by different ethanol solutions and extraction temperature for determining the solubility of the crude extract. After obtaining the extract fractions by using different ethanol concentrations, the antioxidant activity (for DPPH method) was increased about 1.5 times over Pycnogenol as standard material. The extract fractions were tested under an accelerated atmospheres (low, room, and high temperatures) and showed relatively stable under room temperature. The HPLC analysis of the fractions showed that water soluble fractions(Pinexol 3-1 and 3-2) were very similar profiles but water insoluble fraction(Pinexol B) was quite different from the others. Formulation experiments were successfully performed using the water soluble fractions, which not only penetrated through the mouse skin, but also accumulated in the skin layer. For the standard compound for analysis of skin permeation experiment, catechin of the pine bark extract was selected for quantitative HPLC analysis of the fraction. Therefore, gel- and matrix-types of patch could be manufactured using the pine bark extract fraction and the patch test samples were made by a professional patch manufacturer. Functional cosmetic patch products with antiwrinkle, whitening. antibiotic, anti-inflammatory activities will be developed through additional patch research and official certification based on the current research achievements. Moreover, additional medical products for treating skin diseases such as atopy, acne, hives, etc. can be developed by applying this research results.
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