초록 ▼

소송채 CMS에 내병성이 없는 배추 계통을 2회 교잡한 것을 기본 CMS의 재료(공여친)로 이용하고 뿌리혹병과 TuMV 및 무름병의 3대병 복합내병성 순계(DH line) 중 결구력이 약한 A형 계통과 결구력이 강한 B형 계통을 반복친으로 하여 여고잡하였다. 요교잡 과정이 분리세대 개체를 DNA marker로 검정하여 반복친에 가장 가까운 개체를 강선발함으로서 적어도 $BC_3F_1$에서 고정된 CMS 계통을 얻도록 하였다. 그리고 이 CMS 계통에 기존의 다양한 보유 계통을 교잡하여 1대잡종을 만들고 그들의 성

소송채 CMS에 내병성이 없는 배추 계통을 2회 교잡한 것을 기본 CMS의 재료(공여친)로 이용하고 뿌리혹병과 TuMV 및 무름병의 3대병 복합내병성 순계(DH line) 중 결구력이 약한 A형 계통과 결구력이 강한 B형 계통을 반복친으로 하여 여고잡하였다. 요교잡 과정이 분리세대 개체를 DNA marker로 검정하여 반복친에 가장 가까운 개체를 강선발함으로서 적어도 $BC_3F_1$에서 고정된 CMS 계통을 얻도록 하였다. 그리고 이 CMS 계통에 기존의 다양한 보유 계통을 교잡하여 1대잡종을 만들고 그들의 성능을 검정하였다.
한편 바이러스병에 내병성인 순계와 이병성인 순계간의 $F_1$을 소포자 배양하여 얻은 반수성2배체(DH 계통)계통 또는 그 $F_1$에 이병성 친을 교잡하여 얻은 $BC_1F_1$을 이용하여 배추 바이러스병(TuMV-C4)의 내병성 개체 선발용 분자표지인자를 개발하였다.

Abstract ▼

Establishment of a system for rapid development of cytoplasmic male sterile line multi-resistant to club root and viral diseases and development of molecular markers for viral disease in Chinese cabbage This study was consisted of mainly two. subjects. The first was to introduce rapidly a new cytopl

Establishment of a system for rapid development of cytoplasmic male sterile line multi-resistant to club root and viral diseases and development of molecular markers for viral disease in Chinese cabbage This study was consisted of mainly two. subjects. The first was to introduce rapidly a new cytoplasmic male sterility, that is 'Komatsuna CMS', to inbred lines multiple resistant to club-root and turnip mosaic virus diseases of Chinese cabbage, Brassica rapa ssp. pekinensis, with the marker-assisted selection system The other was develop a selection marker for resistance to virus TuMV -C4 in Chinese cabbage.
No available CMS for Chinese cabbag was not identified among the various Brassica CMS such as Ogura of Raphnlls sativus, Anand of B. jllncea, and Polima of B. napus up to the beginning time of this project. All of them did not present the heterosis in the hybrid. The Komatsuna CMS found recently was strongly suggested to be studied because it was originated from the grafted zone of the scion of Komathuna which is the same species as Chinese cabbage and the cabbage stock. It was expected that there was no faults of CMS's studied previously, such as chlorosis under low temperature and no heterosis in the hybrid.
The first trial was carried out to identify that how many prinler pairs for AFLP could be needed in minimum to well-identification of near or remote plants to the recurrent parent(RP). A multiple resistant inbred(doubled haploid line) of B type with the compact head was crossed to unknown line of Komatsuna CMS Chinese cabbage. Ninety FJ pants were subjected to AFLP with 11 primer pairs with the RP. The order of nearness of them to the RP was determined with every primer pair and every combination with 11 pairs. A combination with certain 4 primer pairs almost identical to the 11 pairs combination in the nearness order in this population. The total polymorphic bands of 4 primer pairs were 325 out of 786 of 11 pairs. Thereafter these 4 primer pairs were generally used for selection of near plants to the RP in the further back cross procedure.
The second trial was to identify the effect of the marker-assisted selection in the back cross system. Although the number of plants was very small in certain generation and population because of the low fertility of CMS lines, a fixed CMS inbred in the B type could be established at the BC3Fl, which at least 2-3 generations were reduced compared to the traditional selection technique.
The third matter of concern was that the Komatsuna CMS could be available or not for the Fl hybrid breeding in Chinese cabbage. Most CMS populations derived from the B type line was lowered tremendously in the seed bearing ability from the $BC_1F_1$ generation and showed the severe chlorosis and no heterosis in $F_l$ crosses like those of Ogura CMS. Thus an investigation was carried out to clarify that Komatsuna CMS and Ogura CMS are same or not with the primer for orfl38 gene of Ogura CMS. Surprisingly Komatsuna CMS presented the specific band of orfl38 gene. However, fortunately some other CMS populations of the A type line produced as much seeds as the RP in the BCz and BC3 generations, eventhough they were still segregated in some characteristics because of non selection of the near plants to the RP. In general speaking, Komatsuna CMS is the same as the Ogura CMS. But it could be used for $F_l$ hybrid breeding without chlorosis and no-heterosis by selecting proper materials before or during back cross. The final objective of this project was development of a selection marker for TuMV-C4 resistance in Chinese cabbage. An AFLP marker was first selected from the bulked segregant analysis with DH lines derived from an Fl hybrid made by crossing a resistant line and susceptible inbred. A SCAR marker derived from the AFLP marker was tested with the DH lines and the result was fitted very well. But it was not suitable for the Fz population. The DNA sequences amplified with the primer was analyzed and identified to be a transposon. So it was considered that this primer could not be used as a selection marker for TuMV -C4 resistance. At the applicable time, A total of 238 SSR primers was released from the National Institute of Agricultural Biotechnology, RDA. They were tested with the same previous materials and 3 co-dominant primer pairs were selected finally. KS-TuMV -3, which was named arbitrarily, out of them was found to be linked most closely to TuMV resistant gene with 2.2 cM and located on the 6th linkage group corresponding to chromosome number 4.

목차 Contents

• 제 1 장 연구개발과제의 개요 ...20
• 제 2 장 국내외 기술개발 현황 ...31
• 제 3 장 연구개발 수행 내용 및 결과 ...37
• 제 1 절 배추 복합내병성 CMS 계통 조기 육성과 그 체계 확립 및 분자표지인자 개발 ...37
• 제 2 절 Fox-hunting system을 이용한 배추속 작물의 저발현 기능유전자 대량획득 및 육종소재 창성 ...102
• 제 3 절 애기장대 웅성볼임 변이체를 이용한 임성관련 유전자의 분리 및 이용 ...251
• 제 4 절 S-RNase 유전자의 RNA interference 방법에 의한 자가화합성 사과계통 육성 ...290
• 제 5 절 분자마커를 이용한 무.배추 무사마귀병 저항성 선발마커 개발 ...320
• 제 4 장 목표달성도 및 관련분야에의 기여도 ...335
• 제 5 장 연구개발결과의 활용계획 ...342
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ...348
• 제 7 장 참고문헌 ...352