초록 ▼

본 연구에서는 월별로 넙치의 mucus를 채취하여, mucus내에 있는 다양한 innate immune components의 변화를 조사하고자 함. 조사항목으로 lysozyme의 활성도, alkaline phosphatase와 esterase의 활성도, natural anitbody (IgM), agglutinin, pretense activity등이 있음. 이러한 조사를 통하여 넙치의 계절별 mucus components의 변화와 그에 따른 외부 감염물질의 방어능을 탐색하여 보고, 특히 넙치에 피해를 많이 주고 있는 스쿠치카의 감

본 연구에서는 월별로 넙치의 mucus를 채취하여, mucus내에 있는 다양한 innate immune components의 변화를 조사하고자 함. 조사항목으로 lysozyme의 활성도, alkaline phosphatase와 esterase의 활성도, natural anitbody (IgM), agglutinin, pretense activity등이 있음. 이러한 조사를 통하여 넙치의 계절별 mucus components의 변화와 그에 따른 외부 감염물질의 방어능을 탐색하여 보고, 특히 넙치에 피해를 많이 주고 있는 스쿠치카의 감염을 예방 혹은 치료방법을 모색하고자 함.

Abstract ▼

Olive flounder is an aquatic animal which possess innate and adaptive immune system just like mammals to protect itself from pathogens such as bacteria, virus and parasite in aquatic environment. In the case of fish, slow development of immune system at a fingerling and low temperature condition of

Olive flounder is an aquatic animal which possess innate and adaptive immune system just like mammals to protect itself from pathogens such as bacteria, virus and parasite in aquatic environment. In the case of fish, slow development of immune system at a fingerling and low temperature condition of living habitat limit the role of adaptive immune response. Therefore, the fish has more developed innate immune system. Recent studies reports had been reported of several i late immune related substrates which contained in aquatic animal's ski mucus against adherence and invasion of pathogens. The olive flounder is the most cultured species in Korea, however its culture pose a problem of product loss because of the occurrence of various infectious diseases. This kind of fish produced abundant skin mucus as compared to others but its composition and function was not extensively studied. Therefore, there is a needs to investigate the relationship between infectious disease and mucus components. In this study, the skin mucus of the olive flounder was collected monthly and the changes of the composition of substances was investigated. Furthermore, assays were done on antiparasitic material to replace formalin against scuticocilliate. The collected mucus sample was studied to observe the changes in diverse innate immune components. Lysozyme activity, alkaline phosphatase, esterase activity, natural antibody (IgM), agglutinin and protease activity were conducted. And antiparasitic material was evaluated. The lysozyme activity was in high levels on all the samples and the natural antiboty titer was diverse in the skin mucus. Interestingly, trypsin like pretense was lower against the natural antibody while it higher in the same sample. TDS considerably worked to scuticocilliate but it led to fish toxication. Therefore, it was directly treated to the infected part of the fish. Base on the results, the data obtained can be useful in the understanding of the relatiionship of mucus and infection of diseases. The TDS is enough to be used as antiparasitic agent especially scuticocilliate only if it can be reduce the to)(in and can be easily applied to fish.

목차 Contents

• 제 1 장 연구개발과제의 개요 ...8
• 제 1 절 연구개발 목적 및 필요성 ...8
• 제 2 절 사회.문화적 측면(공공성 포함) ...9
• 제 2 장 국내외 기술개발 현황 및 과학기술정보 ...11
• 제 1 절 국내 및 국외 관련기술 동향 ...11
• 제 2 절 연구결과가 국내.외 기술개발현황에서 차지하는 위치 ...12
• 제 3 장 연구개발수행 내용 및 결과 ...13
• 제 4 장 연구개발 목표달성도 및 관련분야에의 기여도 ...59
• 제 1 절 연도별 연구목표 및 평가착안점에 입각한 연구개발목표의 달성도 ...59
• 제 2 절 관련분야의 기술발전에의 기여도 ...62
• 제 5 장 연구개발결과의 활용계획 ...63
• 제 6 장 참고문헌 ...64