[보고서]자생식물을 이용한 환경친화형 생화학살균제 개발

김진철

보고서 정보

주관연구기관

한국화학연구원
Korea Research Institute of Chemical Technology

연구책임자

김진철

참여연구자

최경자 , 최용호 , 장경수 , 박명수 , 윤미영 , 리당쿠앙 , 조수정 , 백송이

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2010-05

과제시작연도

2009

주관부처

교육과학기술부

사업 관리 기관

한국연구재단

등록번호

TRKO201000011903

과제고유번호

1345097998

사업명

21세기프론티어연구개발사업

DB 구축일자

2015-01-08

키워드

살균활성 검정.생화학살균제.식물추출물.환경친화적 천연물.식물병.Fungicidal bioassay.biochemical fungicide.plant extract.environmentally-friendly natural products.plant disease.

표 대상으로 하는 다섯 가지 식물병에 대한 살균제들의 국내시장
표 “The Manual of Biocontrol Agents(3판)(2004)”에 등록된 식물유래 생물농약
표 소리쟁이로부터 분리한 chrysophanol, nepodin 및 parietin의 오이 흰가루병에 대한 방제 효과
표 소리쟁이 액제(500배 희석액; 왼쪽 사진)와 분말수화제(1,000배 희석액; 오른쪽 사진)의 찔레 꽃잎에 대한 약해.
표 소리쟁이 분말수화제의 멜론 흰가루병에 대한 방제 활성. 왼쪽 사진: 수화제(2,000배 희석액) 처리구, 오른쪽 사진: 무처리구.
표 울금으로부터 분리한 세 가지 curcuminoids의 7가지 식물병에 대한 1일전 예방효과
표 울금 살균제의 고추 탄저병에 대한 포장시험
표 울금 살균제의 고추 흰가루병에 대한 포장시험
표 울금 살균제의 벼 도열병에 대한 포장시험
표 한국화학연구원 생물기능연구팀에서 확립하고 있는 다양한 살균활성 스크리닝 방법
표 한국화학연구원 생물기능연구팀 살균제 연구실의 전천후 유리 온실(왼쪽)과 항온항습실 및 생육선반(오른쪽).
표 Preparative HPLC profile phytosterols isolated from roots of Diopsacus asper. Chromatographic conditions: column, Capcell Pak C18(20 mm × 250 mm); mobile phase, 100% MeOH; flow rate, 10 ㎖/min; detection, 210 nm.
표 GC-MS analysis conditions of compounds isolated from Dipsacus asper roots.
표 Extraction and isolation procedure of antifungal compounds from Dipsacus asper roots.
표 In vivo antifungal activity of the methanol extracts of Dipsacus asper roots and Cynanchum wilfordii roots against seven plant diseasesa)
$In vivo antifungal activity of four fractions obtained by solvent partitioning from a methanol extract of Dipsacus asper roots against seven phytopathogenic fungia)$ 표 In vivo antifungal activity of four fractions obtained by solvent partitioning from a methanol extract of Dipsacus asper roots against seven phytopathogenic fungia)
표 HPLC chromatograms of the three purified compounds 1, 2, and 3 from Dipsacus asper roots.Chromatographic conditions: column, Cosmosil C18(2) (150 mm × 4.6 mm); mobile phase, Methanol 100%; flow rate, 1 ㎖/min; detection, 210 nm and CAD detector.
표 Mass spectra of compound 1(A) isolated from Dipsacus asper roots and library search β-sistosterol(B).
표 Mass spectra of compound 2(A) isolated from Dipsacus asper roots and library search campesterol(B).
표 Mass spectra of compound 3(A) isolated from Dipsacus asper roots and library search stigmasterol(B).
표 Chemical structures of β-sistosterol(A), campesterol(B) and stigmasterol(C).
표 ESI-mass spectrum of compound 4 obtained by positive ion mode.
표 13C-NMR spectrum of compound 4 in methanol-d4 at 125 MHz.
표 1H-NMR spectrum of compound 4 in methanol-d4 at 500 MHz.
표 Chemical structure of cauloside A isolated from Dipsacus asper roots.
표 13C-NMR data of cauloside A isolated from Dipsacus asper.
표 FAB mass spectrum of compound 5 from Dipsacus apser obtained by positive ion mode.
표 13C-NMR spectrum of compound 5 from Dipsacus apser in methanol-d4 at 200 MHz.
표 1H-NMR spectrum of compound 5 from Dipsacus apser in methanol-d4 at 800 MHz.
표 Chemical structure of colchiside isolated from Dipsacus apser.
표 13C-NMR data of colchiside isolated from Dipsacus asper
표 1H-NMR data of colchiside isolated from Dipsacus asper.
표 Growth inhibition of Burkholderia glumae by colchiside isolated from Dipsacus asper.
표 In vivo antifungal activity of five compounds isolated from Dipsacus asper roots against seven plant pathogenic fungia)
표 Suppression of tomato late blight by cauloside A isolated from Dipsacus asper. a=untreated control, b=treated with cauloside A (500 ㎍/ml), c=dimethomorph (10 ㎍/ml).
표 Synergy effects of five compounds isolated from Dipsacus asper roots against seven plant pathogenic fungia)
표 In vivo antifungal activity of various organic solvent extracts of Dipsacus asper roots against seven plant pathogenic fungia)
표 One-day protective activity of liquid concentrate of acetone extract of Dipsacus asper root against 7 plant diseasesa)
$Preparative HPLC profile of F6 fraction from Cynanchum wilfordii roots. Chromatographic conditions: column, Capcell Pak C18 (20 mm × 250 mm); mobile phase, water:acetonitril gradient system; flow rate, 10 ㎖/min; detection, 220 nm and 275 nm.$ 표 Preparative HPLC profile of F6 fraction from Cynanchum wilfordii roots. Chromatographic conditions: column, Capcell Pak C18 (20 mm × 250 mm); mobile phase, water:acetonitril gradient system; flow rate, 10 ㎖/min; detection, 220 nm and 275 nm.
$Preparative HPLC profile of F8 fraction from Cynanchum wilfordii roots. Chromatographic conditions: column, Capcell Pak C18 (20 mm × 250 mm); mobile phase, water:acetonitril gradient system; flow rate, 10 ㎖/min; detection, 220 nm and 275 nm.$ 표 Preparative HPLC profile of F8 fraction from Cynanchum wilfordii roots. Chromatographic conditions: column, Capcell Pak C18 (20 mm × 250 mm); mobile phase, water:acetonitril gradient system; flow rate, 10 ㎖/min; detection, 220 nm and 275 nm.
표 Extraction and isolation of compounds from Cynanchum wilfordii roots.
표 In vivo antifungal activity of the methanol extracts of Dipsacus asper roots and Cynanchum wilfordii roots against seven plant diseasesa)
$In vivo antifungal activity of four fractions obtained by solvent partitioning from a methanol extract of Cynanchum wilfordii root against seven phytopathogenic fungia)$ 표 In vivo antifungal activity of four fractions obtained by solvent partitioning from a methanol extract of Cynanchum wilfordii root against seven phytopathogenic fungia)
표 HPLC chromatograms of the two purified compounds 8 and 9 from Cynanchum wilfordii roots. Chromatographic conditions: column, Cosmosil C18(2) (150 mm × 4.6 mm); mobile phase, water and acetonitril gradient system; flow rate, 1 ㎖/min; detection, 220 nm and 275 nm.
표 HPLC chromatograms of the two purified compounds 8 and 9 from Cynanchum wilfordii roots. Chromatographic conditions: column, Cosmosil C18(2) (150 mm × 4.6 mm); mobile phase, water and acetonitril gradient system; flow rate, 1 ㎖/min; detection, 220 nm and 275 nm.
표 ESI-mass spectrum of compound 6 from Cynanchum wilfordii obtained by positive ion mode.
표 13C-NMR spectrum of compound 6 from Cynanchum wilfordii in methanol-d4 at 125 MHz.
표 1H-NMR spectrum of compound 6 from Cynanchum wilfordii in methanol-d4 at 500 MHz.
표 Chemical structure of wilfoside ClN isolated from Cynanchum wilfordii.
표 ESI-mass spectrum of compound 7 obtained by positive ion mode.
표 13C-NMR spectrum of compound 7 from Cynanchum wilfordii in methanol-d4 at 125 MHz.
표 1H-NMR spectrum of compound 7 from Cynanchum wilfordii in methanol-d4 at 500 MHz.
표 Chemical structure of wilfoside KlN isolated from Cynanchum wilfordii.
표 ESI-mass spectrum of compound 8 from Cynanchum wilfordii obtained by positive ion mode.
표 13C-NMR spectrum of compound 8 from Cynanchum wilfordii in methanol-d4 at 125 MHz.
표 1H-NMR spectrum of compound 8 from Cynanchum wilfordii in methanol-d4 at 500 MHz.
표 Chemical structure of cynauricuoside A isolated from Cynanchum wilfordii.
표 ESI-mass spectrum of compound 9 from Cynanchum wilfordii obtained by positive ion mode.
표 13C-NMR spectrum of compound 9 from Cynanchum wilfordii in methanol-d4 at 125 MHz.
표 1H-NMR spectrum of compound 9 from Cynanchum wilfordii in methanol-d4 at 500 MHz.
표 Chemical structure of wilfoside ClG isolated from Cynanchum wilfordii.
표 LC mass spectrum of compound 10 from Cynanchum wilfordii obtained by positive ion mode.
표 13C-NMR spectrum of compound 10 from Cynanchum wilfordii in methanol-d4 at 125 MHz.
표 1H-NMR spectrum of compound 10 from Cynanchum wilfordii in methanol-d4 at 500 MHz.
표 Chemical structure of wilfoside K1GG isolated from Cynanchum wilfordii.
표 LC mass spectrum of compound 11 from Cynanchum wilfordii obtained by positive ion mode.
표 13C-NMR spectrum of compound 11 from Cynanchum wilfordii in methanol-d4 at 500 MHz.
표 1H-NMR spectrum of compound 11 from Cynanchum wilfordii in methanol-d4 at 500 MHz.
표 Chemical structure of wilfoside C1GG isolated from Cynanchum wilfordii.
표 Growth inhibition of Ralstonia solanacearum by cynauricuoside A isolated from Cynanchum wilfordii.
표 Growth inhibition of Ralstonia solanacearum by wilfoside ClG isolated from Cynanchum wilfordii.
표 Inhibition of mycelial growth of Magnapothe grisea by wilfoside C1N and wilfoside K1N isolated from Cynanchum wilfordii.
표 In vivo antifungal activity of six compounds isolated from Cynanchum wilfordii roots against seven plant pathogenic fungia)
표 Suppression of barley powdery mildew caused by Erysiphe graminis f.sp. hordei by wilfoside C1N and wilfoside C1G isolated from Cynanchum wilfordii. a=untreated control, b, c, d=treated with 500 ㎍/ml, 250 ㎍/ml, and 125 ㎍/ml, respectively, of the purified substance, e=flusilazole (10 ㎍/ml).
표 In vivo antifungal activity of solvent extracts of Cynanchum wilfordii roots against seven plant pathogenic fungia)
표 One day-protective activities of the methanol extracts of stem barks of Magnolia obovata and M. officnialis against seven plant diseases. RCB: rice blast, RSB: rice sheath blight, TGM: tomato grey mold, TLB: tomato late blight, WLR: wheat leaf rust, BPM: barley powdery mildew, RPA: red pepper anthracnose. The methanol extracts were treated at a concentration of 3,000 ㎍/ml.
$One day-protective activity of the four fractions obtained from a methanol extract of Magnolia obovata stem barks against seven phytopathogenic fungi*$ 표 One day-protective activity of the four fractions obtained from a methanol extract of Magnolia obovata stem barks against seven phytopathogenic fungi*
표 Chemical structure of 4-methoxyhonokiol and its NMR spectra.
표 Chemical structure of magnolol and its NMR spectra.
표 Chemical structure of honokiol and its NMR spectra.
표 Chemical structure of obovatol and its NMR spectra.
표 IC50 values of four neolignans isolated from stem barks of Magnolia obovata against nine plant pathogenic fungi*
표 One day-protective activity of neolignans purified from stem barks of Magnolia obovata against seven phytopathogenic fungi*
표 One-day protective activities of various organic solvent extracts of Magnolia officinalis against 7 plant diseases*
표 One day protective activity of the wettable powder type formulation of ethyl acetate extract of Magnolia obovata stem bark against seven plant diseases. RCB: rice blast, RSB: rice sheath blight, TGM: tomato grey mold, TLB: tomato late blight, WLR: wheat leaf rust, BPM: barley powdery mildew, RPA: red pepper anthracnose.
표 One-day protective activities against 7 plant diseases of 6 extracts of Magnolia officinalis which were prepared by two companies in China*
표 One-day protective activities against 7 plant diseases of two formulations of extract (sample 3) of Magnolia officinalis stem bark*
표 Composition of three emulsifiable concentrate type formulations of extract of Magnoloia officinalis stem bark
표 One-day protective activities of four formulations of extract (sample 3) of Magnolia officinalis stem bark against 7 plant diseases *
표 Phytotoxic symptoms caused by wettable powder type formulation of extract of Magnolia officinalis stem bark.
표 Quantification of magnolol and honokiol from three extracts of Magnolia officinalis stem bark which contains the two compounds by 50%, 80%, and 90%, respectively
표 HPLC chromatograms of three extracts of Magnolia officinalis stem bark.
표 Contents of magnolol and honokiol in the samples which were stored at 54℃ for different periods.
표 One-day protective activities against 7 plant diseases of wettable powder type formulation samples of extract (sample 3) of Magnolia officinalis stem bark which were stored at 54℃ during different periods*
표 Protective activity of wettable powder type formulation of extract of Magnolia officinalis stem bark against strawberry anthracnose
표 Control efficacy of wettable powder type formulation of the extract of Magnolia officinalis stem bark against potato late blight in field(a: treated, b: untreated).
표 Compositions of two wettable powder type formulations (WP-25 and WP-26) of the extract of Rumex crispus root
표 Control efficacy of wettable powder type formulations of the extract of Rumex crispus root against cucumber powdery mildew in greenhouse
표 Control efficacy of a wettable powder type formulation(WP-25) of the extract of Rumex crispus root against melon powdery mildew in a field of Seongju, Kyoungbuk province
표 Control efficacy of a wettable powder type formulation(WP-25) of the extract of Rumex crispus root against melon powdery mildew in a field of Chilgok, Kyoungbuk province
표 Control efficacy of a wettable powder type formulation(WP-25) of the extract of Rumex crispus root against cucumber powdery mildew in a field of Daejeon
표 Control efficacy of a wettable powder type formulation(WP-25) of the extract of Rumex crispus root against strawberry powdery mildew in a field of Nonsan, Chungnam province
표 Quantification of nepodin, emodin, chrysophanol, and parietin in various extracts of Rumex crispus and Rheum undulatum roots by HPLC
표 HPLC chromatograms of various extracts of Rumex crispus and Rheum undulatum
표 One-day protective activity of several preparations of wettable powder type formulation of Rumex crispus and Rheum undulatum roots against barley powdery mildew
표 Results of acute toxicity tests of a natural fungicide,"chryetin (emodin 3.2% + parietin 0.4%), which is active to powdery mildews
표 Composition of wettable powder type formulation of the ethyl acetate extract of Curcuma longa rhizome
표 Composion of new wettable type formulation of tumeric
표 Composition of wettable type formulation of a mixture of tumeric and DD chemical
표 Control efficacy of a wettable powder type formulation(WP-23) of the ethyl acetate extract of Curcuma longa rhizome against strawberry powdery mildew in a field of Nonsan, Chungnam province
표 Control efficacy of a wettable powder type formulation(WP-23) of the ethyl acetate extract of Curcuma longa rhizome against apple blotch in a field of Whaseong, Chungnam province
표 Control efficacy of a wettable powder type formulation(WP-23) of the ethyl acetate extract of Curcuma longa rhizome against pepper anthracnose in a field of Whaseong, Chungnam province
표 Control efficacy of a wettable powder type formulation(WP-23) of the ethyl acetate extract of Curcuma longa rhizome against rice leaf blast in a field of Whaseong, Chungnam province
표 One-day protective activities against 7 plant diseases of wettable powder type formulations of several tumeric samples which were imported from India and China*
표 HPLC chromatograms of various tumeric samples which were imported from India
표 Quantification of three curcuminoids in various tumeric samples which were imported from India by HPLC
표 One-day protective activities against 7 plant diseases of two different formulations of tumeric (India-1) which were imported from India
표 Contents of three curcuminoids in the diffusible concentrate type formulation of tumeric which were stored at 45℃ for different periods
표 One-day protective activities against 3 plant diseases of the diffusible concentrate type formulation of tumeric which were stored at 45℃ for different periods*
표 One-day protective activities of various preparations of tumeric against 7 plant diseases
표 Control efficacy of various preparations of tumeric against rice head blast
표 Composition of wettable powder type formulation of the hexane/ethyl acetate extract of Myristica fragrans seed
표 One-day protective activities of various organic solvent extracts of Myristica fragrans seed against 4 plant diseases
표 Phytotoxicity of various organic extracts of Myristica fragrans seed on strawberry plants
표 One-day protective activities of wettable powder type formulations of methanol (WP-23) and hexane/ethyl acetate (WP-24) extracts of Myristica fragrans seed against 7 plant diseases
표 Control efficacy of a wettable type formulation (WP-24) of Myrisitica fragrans seed against pepper anthracnose in a field of Sangju, Kyoungbuk province
표 Control efficacy of a wettable type formulation (WP-24) of Myrisitica fragrans seed against rice leaf blast in a field of Sangju, Kyoungbuk province
표 One-day protective activities of the methanol extracts of Myristica fragrans seed (nutmeg) and Myristica malabarica fruit rind (Bombay mace) against 7 plant diseases
표 Persistence activity of the methanol extract of Myrisitica malabarica fruit rind against rice blast
표 Persistence activity of the methanol extract of Myrisitica malabarica fruit rind against tomato late blight
표 Persistence activity of the methanol extract of Myrisitica malabarica fruit rind against wheat leaf rust
표 Persistence activity of the methanol extract of Myrisitica malabarica fruit rind against pepper anthracnose
표 The 1H-NMR and 13C-NMR spectrum of 3β-acetyl-20S-hydroxy dammarane-24-ene.
표 The 1H-NMR and 13C-NMR spectrum of phylligenin.
표 The 1H-NMR and 13C-NMR spectrum of pinoresinol .
표 The 1H-NMR and 13C-NMR spectrum of lariciresinol
표 One-day protective activity of compounds isolated from Forsythia Fructus
표 The 1H-NMR and 13C-NMR spectrum of quercetrin .
표 The 1H-NMR and 13C-NMR spectrum of miricitrin .
표 The 1H-NMR and 13C-NMR spectrum of 1,2,6-trigalloylglucopyranoside.
표 Ode-day protective activity of compounds from Juglans mandshurica
표 The 1H-NMR and 13C-NMR spectrum of wogonin.
표 One-day protective activity of wogonin from Scutellaria baicalensis
표 The 1H-NMR and 13C-NMR spectrum of cinnamaldehyde.
표 One-day protective activity of cinnamaldehyde from Cinnamomum cassia
표 The 1H-NMR and 13C-NMR spectrum of 3β-acetoxy-28-hydroxyolean-12-ene.
표 The 1H-NMR and 13C-NMR spectrum of 3β-acetoxy-12-en-28-acid.
표 The 1H-NMR and 13C-NMR spectrum of taraxenol.
표 The 1H-NMR and 13C-NMR spectrum of stigmasterol.
표 Frationation of Stem-bark of C. japonica.
$Isolation of compounds from hexane and EtOAc-soluble fractions.$ 표 Isolation of compounds from hexane and EtOAc-soluble fractions.
표 The 1H-NMR and 13C-NMR spectrum of compound 1.
표 The 1H-NMR and 13C-NMR spectrum of compound 2.
표 The 1H-NMR and 13C-NMR spectrum of compound 3.
표 The 1H-NMR and 13C-NMR spectrum of compound 4.
표 The 1H-NMR and 13C-NMR spectrum of compound 5.
표 The 1H-NMR and 13C-NMR spectrum of compound 6.
표 The 1H-NMR and 13C-NMR spectrum of compound 7.
표 The 1H-NMR and 13C-NMR spectrum of compound 8.
표 The 1H-NMR and 13C-NMR spectrum of compound 9.
표 The 1H-NMR and 13C-NMR spectrum of compound 10.
표 One-day protective activity of compounds from Camellia japonica
표 The 1H-NMR and 13C-NMR spectrum of berberine.
표 One-day protective activity activity of berberine from Coptidis Rhizoma
표 Fractionation of Chelidonium majus.
$Isolation of compounds from hexane and EtOAc-soluble fractions of C. majus.$ 표 Isolation of compounds from hexane and EtOAc-soluble fractions of C. majus.
표 The 1H-NMR and 13C-NMR spectrum of compound 1.
표 The 1H-NMR and 13C-NMR spectrum of compound 2.
표 The 1H-NMR and 13C-NMR spectrum of stylopine.
표 The 1H-NMR and 13C-NMR spectrum of compound 4.
표 The 1H-NMR and 13C-NMR spectrum of berberine.
표 The 1H-NMR and 13C-NMR spectrum of 8-hydroxydihydrosanguinarine.
표 One-day protective activity of compounds from Chelidonium majus
표 Cinnamylaldehyde의 화학구조.
표 L-glutamic acid(왼쪽)와 γ-aminobutyric acid(오른쪽)의 화학구조.

과제명(ProjectTitle) :	-
연구책임자(Manager) :	-
과제기간(DetailSeriesProject) :	-
총연구비 (DetailSeriesProject) :	-
키워드(keyword) :	-
과제수행기간(LeadAgency) :	-
연구목표(Goal) :	-
연구내용(Abstract) :	-
기대효과(Effect) :	-

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 제목(한글), 저자명(한글), 발행일자, 전자원문, 초록(한글), 초록(영문) 관리번호, 제목(한글), 제목(영문), 저자명(한글), 저자명(영문), 주관연구기관(한글), 주관연구기관(영문), 발행일자, 총페이지수, 주관부처명, 과제시작일, 보고서번호, 과제종료일, 주제분류, 키워드(한글), 전자원문, 키워드(영문), 입수제어번호, 초록(한글), 초록(영문), 목차
저장형식	Text(ASCII format) Excel format
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

자생식물을 이용한 환경친화형 생화학살균제 개발
Development of Eco-friendly Biochemical Fungicides Using Higher Plant-derived Natural Products 원문보기

초록 ▼

Abstract ▼

목차 Contents

표/그림 (175)

표/그림 (175)

연구자의 다른 보고서 :

참고문헌 (25)

연구과제 타임라인

관련 콘텐츠

원문 보기

이 보고서와 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

자생식물을 이용한 환경친화형 생화학살균제 개발 Development of Eco-friendly Biochemical Fungicides Using Higher Plant-derived Natural Products 원문보기

초록 ▼

Abstract ▼

목차 Contents

표/그림 (175) 모든 표/그림 보기

표/그림 (175) 슬라이드로 보기

연구자의 다른 보고서 :

김진철 (37) 최경자 (45) 최용호 (28) 장경수 (29) 조수정 (1)

참고문헌 (25)

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 보기

이 보고서와 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

자생식물을 이용한 환경친화형 생화학살균제 개발
Development of Eco-friendly Biochemical Fungicides Using Higher Plant-derived Natural Products 원문보기

표/그림 (175)

표/그림 (175)