[보고서]동물 종에 따라 감염된 Anaplasma phagocytophilum의 유전자 다양성

채준석

[국가R&D연구보고서] 동물 종에 따라 감염된 Anaplasma phagocytophilum의 유전자 다양성
Genetic diversity of Anaplasma phagocytophilum infected from animal species 원문보기

보고서 정보
주관연구기관	서울대학교 Seoul National University
연구책임자	채준석
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2010-04
과제시작연도	2009
주관부처	교육과학기술부
연구관리전문기관	한국연구재단 National Research Foundation of Korea
등록번호	TRKO201000013098
과제고유번호	1345104354
사업명	일반연구자지원
DB 구축일자	2013-04-18
키워드	기후변화.환경변화.매개질병.진드기.리케치아.과립구성 아나플라스마증.아나플라스마 파고사이토필름.보균동물.유전자 다양성.Climate change.Environmental change.Vector-borne disease.Tick.Rickettsia.Granulocytic anaplasmosis.Anaplasma phagocytophilum.Reservoir animal.Genetic diversity.

초록

지구온난화로 인한 환경변화는 절지동물 매개체 및 보균동물과 숙주동물의 환경에 영향을 주어 새로운 질병 발생을 증가시키고 있다. 국내 기후가 아열대로 변화되어 가고있는 과정에 진드기 매개성 리케치아성 질병인 과립구성 아나플라스마증(granulocytic anaplasmosis)의 원인체인 Anaplasma phagocytophilum 병원체를 보유한 숙주, 매개체 및 보균동물 종간에 따른 병원성과 항원성 인자들의 유전적 다양성을 규명하고, 감염경로와 예방을 위한 지도를 작성하고자 하였다.

Abstract ▼

Recently, emerging diseases are increasing according to environmental changes due to global warming and the change of arthropod vectors, reservoirs and host animals. Korean climate is changing to subtropical climate in the meantime, tick-borne rickettsial granulocytic anaplasmosis causes by Anaplasma phagocytophilum detected from vector ticks and wild rodents and other mammals. Some of the genetic sequences of pathogens are revealed. A. phagocytophilum has related to genetic diversity of the pathogenicity and antigenicity depend on vector species, carrier animals and/or host animal species are related to whether you want to identify and mapping for the infection route and prevention.

목차 Contents

일반연구자지원사업 최종보고서(초안) ...1
목차 ...3
Ⅰ. 연구 계획 요약문 ...4
1. 국문 요약문 ...4
Ⅱ. 연구 결과 요약문 ...5
1. 국문 요약문 ...5
2. 영문 요약문 ...6
Ⅲ. 연구내용 및 결과...7
1. 연구 개발 과제의 개요 ...7
2. 국내.외 기술 개발 현황 ...8
3. 연구 수행 내용 및 결과 ...10
4. 목표 달성도 및 관련 분야에의 기여도 ...38
5. 연구 결과의 활용 계획 ...39
6. 연구 과정에서 수집한 해외 과학기술 정보 ...41
7. 주관연구책임자 대표적 연구 실적 ...41
8. 참고 문헌 ...41
9. 연구 성과 ...45
10. 기타사항...45

표/그림 (35)

표 Diagram of sample collection from Korean water deer.
표 Tick collection sites in Jeju-Do (A) and US Army base and training sites in korea (B).
표 Tick collection from migratory birds and wild rodents by Korea National Park Migratory Bird Center (MBC) and 18 MEDCOM, US Army at Hong-do.
표 Birds with ticks attached to the neck (A) Haemaphysalis flava (arrow) on Emberiza elegans (Yellow-throated Bunting), and around the eye (B) Ixodes nipponensis (arrow) on Zoothera sibirica (Siberian Thrush).
표 Birds with tick attached to the nead, Haemaphysalis ornithophila (arrow, first documented in Korea) on Zoothera dauma (White' s ground thrush).
표 Identification of tick species by stereomicroscope.
표 Oligonucleotide sequences of PCR primer sets for amplification of Haemaphysalis longicornis tick, A. phagocytophilum and A. bovis
표 Condition of PCR reaction for H. longicornis tick, A. phagocytophilum and A. bovis
표 Agarose gel electrophoresis of H. longicornis tick 5.8S rRNA internal transcribed spacer 2 gene (360 bp amplicons). Lane M, 100 base pair size marker. Abbreviation of each lane is as follows: W, water control; LA, larva; NY, nymph; AM, adult male; AF, adult female.
표 Comparative analysis between A. phagocytophilum (A) and A. bovis (B) 16S rRNA gene fragments by PCR. Lane 1-6, samples; P, posive control (A. phagocytophilum Webster strain, USA); N, negative control, M. DNA size marker (100 bp DNA Ladder, Elpisbiotech).
표 PCR amplicons of A. phagocytophilum groEL (A), msp2 (B) and ankA (C) gene fragments. Lane 1-7, samples; P, posive control (A. phagocytophilum Webster strain, USA); N, negative control, M. DNA size marker (100 bp DNA Ladder, Elpisbiotech).
표 PCR-RFLP (Restriction Fragment Length Polymorphism) pattern for identification of A. phagocytophilum genotypes. (A) 16S rRNA gene (926 bp) with Hinf1 digested pattern. (B) groEL (1,297 bp) gene with Hae3 digested pattern. AP1, Korean major type 1, 2 and 3; PC, positive control (Webster strain, USA, type 4, 5 and 6; APg1 and APg2, samples; M, DNA size marker (100 bp DNA Ladder, Elpisbiotech).
표 Collected samples for detection of A. phagocytophilum from the Conservation Genome Resource Bank (CGRB) for Korean Wildlife in Seoul National University, 18 MEDCOM US Army in Korea, Korea National Park Migratory Bird Center (MBC), and Institute of Environmental Resource Research (IERR) of Jeju Special Self-Governing Province, Seoul National University Animal Hospital (SNUAH)
표 The collected samples from the Conservation Genome Resource Bank for Korean Wildlife
표 The collected tick samples from carcass of wild animals at the Conservation Genome Resource Bank for Korean Wildlife
표 Regional distribution of A. phagocytophilum and A. boivs for collected Korean water deer.
표 The collected samples from the Conservation Genome Resource Bank for Korean Wildlife during 2008 to 2009
표 Ticks collected from wild rodents at US army installations and training sites in Korea in 2008
표 Detection of A. phagocytophilum from wild rodents collected at US Army training sites in 2008.
표 Ticks collected from US army training sites in Korea in 2008
표 Ticks collected from Korea National Park Migratory Bird Center (MBC) at Hong-do
표 Detection of Anaplasma species from ticks collected from migratory birds, cat and Siberian Weasel in Hong-do, Republic of Korea, 2008-2009.
표 Prevalence of total ticks (number of pools) and tick-borne pathogens identified from the three main survey areas during 2007 and 2008 in Jeju Island, Korea
표 Map of Jeju Island, Korea. Seventy-two tick collection sites indicated by black dots are classified into three areas (East, West, and North) and tick-borne pathogens identified in Jeju Island are indicated by squares.
표 Phylogenetic tree based on 1,406 bp sequences of Anaplasma and Ehrlichia species collected in Jeju Island of Korea. The phylogenetic tree was constructed based on the alignment of 16S rRNA gene sequences by CLUSTAL W and followed by the unweighted pair group method with arithmetic mean (UPGMA) method with 1,000 bootstrap resamplings using MEGA software. The GenBank accession numbers are in parentheses.
표 Phylogenetic tree based on 926 bp gene sequence of A. phagocytophilum, A. bovis, and A. centrale. The phylogenetic tree was constructed based on the alignment of Anaplasma gene sequences obtained from species-specific nested PCR assay, by CLUSTAL W and followed by the UPGMA method with 1,000 bootstrap resamplings using MEGA software.
표 Phylogenetic tree based on 390 bp sequence of E. chaffeensis. The phylogenetic tree was constructed based on the alignment of Ehrlichia gene obtained from species-specific nested PCR assay, by CLUSTAL W and followed by the UPGMA method with 1,000 bootstrap resamplings using MEGA software.
표 Prevalence of A. phagocytophilum in ticks and animals in Korea, 2008-2009
표 Phylogenetic analysis for Anaplasma species based on 16S rRNA gene sequences from Korea.
표 Phylogram of the Anaplasmataceae based on 16S rRNA gene sequence (926 bp). Red colors shown new identified sequence types of A. phagocytophilum.
표 Phylogram of the Anaplasmtaceae based on 409 amino acid sequences of groEL gene. Red colors shown new identified sequence type of A. phagocytophilum (box).
표 Phylogram of the Anaplasmtaceae based on 151 amino acid sequences of msp2 gene. Red colors shown new identified sequence type of A. phagocytophilum (box).
표 Phylogram of the Anaplasmataceae based on 137 amino acid sequences of ankA gene. Red colors shown new identified sequence types of A. phagocytophilum (box).
표 Map of Republic of Korea with detection of A. phagocytophilum from Korean water deer, mammals, Wild rodents and ticks. Marking on out of land are unknown areas of samples.
표 Map of Republic of Korea with overlap marking for detection of A. phagocytophilum from different provinces. Marking on out of land are unknown areas of samples.

참고문헌 (25)

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[국가R&D연구보고서] 동물 종에 따라 감염된 Anaplasma phagocytophilum의 유전자 다양성
Genetic diversity of Anaplasma phagocytophilum infected from animal species 원문보기