l. 연구내용 (1) 포도씨유 조제: 용매추출법， 압착법， $CO_{2}$ 초임계추출법 (2) 포도씨 메탄올추출물 조제: 포도 품종별， 산지별 (3) Polyamide co!umn chromatography를 이용한 바닐린비색법의 개선 (5) 바닐린비색법 및 HPLC에 의한 포도씨유 및 추출물 그리고 가공식품의 catechin류 정량분석 2. 연구범위 (1) 개량 바닐린비색법 및 HPLC에 의한 포도 품종별 및 산지별 포도씨유 및 메탄올추출물 의 catechin류 함량 분석
l. 연구내용 (1) 포도씨유 조제: 용매추출법， 압착법， $CO_{2}$ 초임계추출법 (2) 포도씨 메탄올추출물 조제: 포도 품종별， 산지별 (3) Polyamide co!umn chromatography를 이용한 바닐린비색법의 개선 (5) 바닐린비색법 및 HPLC에 의한 포도씨유 및 추출물 그리고 가공식품의 catechin류 정량분석 2. 연구범위 (1) 개량 바닐린비색법 및 HPLC에 의한 포도 품종별 및 산지별 포도씨유 및 메탄올추출물 의 catechin류 함량 분석 (2) 포도씨유 제조 방법에 따른 catechin류 함량 분석 (3) 포도씨유 및 추출물 함유 가공식품의 catechin류 함량 분석
Abstract▼
This study was conducted to develope new HPLC method in place of a conventional vanillin spectrophotometrical method for quantitative analysis of catechin derivatives(catechins and procyanidins) in grape seed oils and extract, and in many functional foods. Additionally, theimproved vanillin spectro
This study was conducted to develope new HPLC method in place of a conventional vanillin spectrophotometrical method for quantitative analysis of catechin derivatives(catechins and procyanidins) in grape seed oils and extract, and in many functional foods. Additionally, theimproved vanillin spectrophotometrical method using a polyamide column chromatography, which enabled to eliminate unidentified major polymeric proanthocyanidins in grape seeds, which were greatly influenced on quantification of catechins, also developed. For the improved vanillin spectrophotometric method, grape seed methanol extract had considerable catechin content in the range of 0.5~3.0%(dry base), while grape seed oils had very small amounts(below 5 ppm) of catechins. Meanwhile, the HPLC analysis was carried out using a reversed-phase YMC-Pack Pro C18, UV absorbance detector set at 280 nm and data module. Separation was performed using linear gradient elution from solvent A(0.5% formic acid) to solvent B(90% aqueous acetonitrile) for 60 min. For the HPLC method, the reproducibility, given as coefficient of variation, was ~5%, and the recovery close to 100%. The achieved detection level of catechins was 1~5 ppm. Four catechins[(+)-catechin, (-)-epicatechin, procyanidin B2 and epicatechin gallate] accounted for above 90% of the methanol extract of grape seeds. The catechin monomers (+)-catechin and (-)-epicatechin were present in greater quantities than any of catechins, and procyanidin B2 and epicatechin gallate were the most abundant procyanidin and galloylated catechins. The range of concentrations(g/l00g of dried seeds) were as follows: (+)-catechin, 0.1~1.9; (-)-epicatechin, 0.1~0.6; procyanidin B2, 0.02~0.09; epicatechin gallate, 0.01~0.04, although there was somewhat difference in catechin contents in relation to grape cultivars, grape-producing areas, andextraction methods of oils. In contrast, the grape seed oils contained very small amounts of two catechins[(+)-catechin and (-)-epicatechin] in the range of 1~5 mg/kg. Finally, moderate levels of two catechins [(+)-catechin and (-)-epicatechin] (15~65 mg%) were measured In Longlife, Hongsamjung, Healthjoint, Propolis, and higher contents(1.03~1.67%) In grape seed extract, medigod and shark grapine, while Yakult 400 and silf had relative small amouts of catechins. Thus, the HPLC method with acceptable extraction and Quantification efficiency is simple, accurate and reproducible for quantification of two catechins and procyanidins from grape seed extracts and oils, as well as many functional foods.
목차 Contents
표지 ...1
용역연구개발사업 최종보고서 ...2
제출문 ...4
요약문 ...5
영문요약문 ...9
목차 ...10
제l장 서론 ...11
제2장 국내.외 기술개발 현황 ...14
제3장 연구개발 수행 내용 및 결과 ...15
가. 연구개발수행 내용 ...15
나. 연구개발수행 결과 ...21
1. HPLC 에 의한 포도씨 메탄올추출물의 catechin류 분석 ...21
2. HPLC 에 의한 포도씨유 catechin류 분석 ...28
3. 비색법 및 HPLC 방법에 의해 측정된 포도씨유의 catechin 함량 비교 ...29
4. 비색법 및 HPLC 방법에 의해 측정된 포도씨 메탄올추출물의 catechin 함량 비교 ...32
5. HPLC 에 의한 포도씨 메탄올추출물의 4가지 주된 catechin류 조성 함량 비교 ...34
6. 비색법 및 HPLC 방법에 의해 측정한 포도씨 가공식품의 catechin 함량 비교 ...36
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