초록 ▼

- 실리카틴 대치용 실리카 및 키토산을 기본으로 하는 단백질분해효소 물리적 고정화 : 자유효소의 50% 성능, 안정성 150%
- 나노웹 위에 단백질분해효소 화학적 고정화 : 자유효소의 50% 성능, 안정성 200%
- 고정화된 단백질분해효소에 의한 세리신 분해 (올리고펩타이드 제조) 올리고펩타이드 분자량 3kDa 이하
- 올리고펩타이드 농축을 위한 분획분자량 2000 이하의 한외여과막 제조 분획분자량 2000, 1000ppm의 PEG 1000 투과율 (3bar에서 4.3$m^{3}$/$

- 실리카틴 대치용 실리카 및 키토산을 기본으로 하는 단백질분해효소 물리적 고정화 : 자유효소의 50% 성능, 안정성 150%
- 나노웹 위에 단백질분해효소 화학적 고정화 : 자유효소의 50% 성능, 안정성 200%
- 고정화된 단백질분해효소에 의한 세리신 분해 (올리고펩타이드 제조) 올리고펩타이드 분자량 3kDa 이하
- 올리고펩타이드 농축을 위한 분획분자량 2000 이하의 한외여과막 제조 분획분자량 2000, 1000ppm의 PEG 1000 투과율 (3bar에서 4.3$m^{3}$/$m^{2}$day, 제거율 20%)
- 효소고정화 막반응기 설계
- Comb polymer 합성 및 분리막 코팅 PEG가 곁가지로 달린 PMMA 합성, 코팅시 130%의 내오염도 향상
- 마르세이유 비누 및 세리신 함유 공정수에서 마르세이유 비누 제거 80% 제거율
- 분자량 3000 이하의 올리고펩타이드 농축 올리고펩타이드 수용액 0.5%에서 5%까지 농축
- 나노웹을 이용한 효소 반응기 설계
- 저분자 세리신의 분획 (분자량 3,000 이상 또는 이하) 및 항산화 평가

Abstract ▼

III. Contents and Area of the Research
1) Enzyme-immobilization by cationic surfactant instead of silaffin
Preparation of highly activated immobilized enzyme used by cationic surfactant Reaction temperature, normal temperature; enzyme concentration, concentration change of silica precursor suc

III. Contents and Area of the Research
1) Enzyme-immobilization by cationic surfactant instead of silaffin
Preparation of highly activated immobilized enzyme used by cationic surfactant Reaction temperature, normal temperature; enzyme concentration, concentration change of silica precursor such as TEOS (TMOS); concentration change of hydrochloric acid and ammonia; concentration change of cationic surfactant; change of reaction time; measuring an enzyme activity; measurement of the particle size and characterization of immobilized enzyme using a SEM; comparison of activity of free enzyme and immobilizaed enzyme; absorbance measurement analyzed by UV-Vis spectrophotometer enzyme stability > 30 days; a singular particle size of immobilized enzyme > 0.5$\mu$m; activity > free enzyme (80%)
2) Maximization of surface area for a nano-web using an enzyme immobilization electro-spinning of nano-web; enzyme immobilization; maximization of enzyme efficiency within the unit time; measurement of activity in the same method 1) enzyme stability > 30 days; activity > free enzyme (50%); preparation of oligopeptide by combined protease
3) Preparation of oligopeptide combined a protease
The combination of proteases (Trypsin, Chymotrypsin, papain, etc.); oligopeptide MWCO measurement; model protein (Bovine serum albumin); oligopeptide MWCO < 500Da
4) Preparation condition of Amphilphilic Comb polymer
Synthesis of comb-polymer (30% polyethyleneglycol); preparation of amphiphilic polymer (methyl methacrylate and polyethylene glycol blocked methyl methacrylate); removal of polymerization prohibitor using a rotary evaporator and column; preparation of random copolymer using solvent (THF) and polymerization initiator (AIBN). The prepared comb polymer should be soluble in a mixed solvent (water and ethanol) MWCO > 100,000Da; PDI < 2
5) Design of membrane coated by comb polymer to mitigate membrane fouling
Establishment of nano membrane condition coated by Amphilphilic Comb polymer Comparision of non-coated nanomembrane; nanomembrane, NF 270; concentration change of comb polymer; coating condition unaffected to premeability MWCO < 150Da; permeate flux > 1$m^{3}$/$m^{2}$day (70psi, 2000ppm $MgSO_{4}$)
6) Preparation of ultrafiltration membrane (MWCO 2000) to permeate oligopeptide
Preparation of polyamide membrane by interfacial polymerization; preparation of hydrophilic UF membrane MWCO > 2000Da; permeate flux > 3$m^{3}$/$m^{2}$day (14.7psi, 1000ppm PEG 2000)
7) Optimization of immobilized bead and membrane reactor
Combination of immobilized bead and UF/NF membrane for a mitigation of membrane fouling; optimization of continuous reactor for model protein solution During the filtration of 1% model protein solution, a permeate was collected; filtration of UF membrane coated by comb polymer; concentration of permeability > oligopeptide(10%); permeate flux > 5 $m^{3}$ /$m^{2}$day; maximization of concentration factor > 5 times
8) Development of refining method by membrane process in protein degummed water
Separation of protein using membrane filtration; permeability efficiency; preparation of UF hollow fiber PVDF membrane (external diameter: 1.6mm, internal diameter: 1.0mm) MWCO > 50,000; soap removal ratio > 90%
9) Design of a membrane reactor for a model protein and a degummed protein
Preparation of oligopeptide using an immobilized enzyme; design of reactor (Packed bed reactor and Fluidized bed reactor) 20L/day
8) Development of refining method by membrane process in protein degummed water
Separation of protein using membrane filtration; permeability efficiency; preparation of
UF hollow fiber PVDF membrane (external diameter: 1.6mm, internal diameter: 1.0mm)
MWCO > 50,000; soap removal ratio > 90%
9) Design of a membrane reactor for a model protein and a degummed protein
Preparation of oligopeptide using an immobilized enzyme; design of reactor (Packed bed
reactor and Fluidized bed reactor) 20L/day
10) Concentration of oligopeptide by membrane filtration
Pressure 2kgf/$cm^{2}$; flow rate 1L/min ; concentration factor > 5 times
11) Evaluation of partition and activity for a low molecular sericin
Evaluation of antioxidized efficiency; measurement of removal efficiency for DPPH; removal efficiency of superoxide anion; coordinate covalent bond of metallic ion amount of TBARS
12) Design of enzyme reactor using nanofiber web as enzyme support
Control of sericin molecular weight as a function of retention time, reaction temperature, and combination of more than two enzyme
13) Application of a low molecular sericin
Comparison of commercially and experimentally antioxided efficiency for a low molecular sericin; application of food and cosmetic industry's additive

목차 Contents

• 제출문 ...1
• 보고서 초록 ...2
• 요약문 ...3
• SUMMARY ...8
• CONTENTS ...14
• 목차 ...15
• 그림목차 ...16
• 표목차 ...19
• 제1장 연구개발과제의 개요 ...20
• 제2장 국내외 기술개발 현황 ...23
• 2-1 국외 ...23
• 2-2 국내 ...25
• 제3장 연구개발수행 내용 및 결과 ...27
• 3-1 연구개발현황 ...27
• 3-2 연구개발내용 ...40
• 3-2-1 단백질 분해 효소 고정화 ...40
• 3-2-2 분획분자량 2000 한외여과막 제조 ...55
• 3-2-3 고정화 비드제조 및 효소고정화 연속반응기 운전 ...58
• 3-2-4 나노웹 효소고정화 및 평가 (위탁연구) ...63
• 3-2-5 Comb polymer의 합성 및 분리막 코팅 (위탁연구) ...69
• 3-2-6 비누 / 단백질 분리 ...83
• 3-2-7 PVDF 중공사막 ...85
• 3-2-8 올리고펩타이드 농축 ...90
• 3-2-9 효소 반응기의 성능평가 (위탁연구) ...91
• 3-2-10 저분자 세리신의 항산화능 (위탁연구) ...97
• 3-2-11 Comb polymer를 이용한 분리막의 내오염성 향상 (위탁연구) ...98
• 3-3 결론 ...104
• 제4장 목표 달성도 및 관련분야에의 기여도 ...105
• ○ 1차년도 ...105
• ○ 2차년도 ...106
• 제5장 연구개발결과의 활용계획 ...107
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ...108
• 제7장 참고문헌 ...112