초록

본 기관에서 제조한 세라믹 기반의 칼슘 포스페이트 골시멘트는 단백질 변형이 없는 저온 상탱에서의 제조 기술로 만들어지며, 이는 플라즈마 이온 코팅법 등 복합 저온 코팅법은 여러가지 방법이 개발되어 있으나 저온 세라믹 골이식재 제조 기술은 전무한 상태에서 생물학적 인자를 변형없이 담지 가능하게 한 기술개발은 매우 우수한 결과임. 생분해속도가 매우 느린 HA 계열의 칼슘 포스페이트 골시멘트가 아닌 생분해성 골이식재로서 담지된 생물학적 인자를 분해 속도 차이에 따라 방출 속도를 조절할 수 있는 것을 확인함.

Abstract ▼

1. Background and necessity of research and development project
■ Increase demand of bone grafts
■ Kinds and (dis)advantages of bone grafts
○ The bone grafts are commonly classified to autografts, allografts, xenografts, and alloplasts.
○ The autografts are ideal bone grafts. there are l

1. Background and necessity of research and development project
■ Increase demand of bone grafts
■ Kinds and (dis)advantages of bone grafts
○ The bone grafts are commonly classified to autografts, allografts, xenografts, and alloplasts.
○ The autografts are ideal bone grafts. there are limitations, including the increased operative time.
limited availability and significant morbidity related to blood loss, wound complications, local sensory loss and. most importantly. chronic pain.
○ Allograft is the most frequently chosen bone substitute and is regarded as the surgeon's second option. Although the risk of transmission of disease is much lower than with blood products. it is still possible.
○ Allogenic bone material carries the potential risk of transmitting tumor cells and a variety of bacterial and viral infections including those that cause AIDS or hepatitis in patients.
○ In recent years, a variety of materials have been investigated and commercially available as alternatives to autogenous bone graft, including xenograft, xenograft derived hydroxyapatite, synthetic calcium phosphates, bioactive glass/glass ceramics. Type I collagen, degradable polymers, several matrix proteins, purified growth factors, isolated bone marrow cells, and composites of those materials.
■ The need of bone growth factors loaded bone grafts which have osteoinductivity
■ The challenges for synthesizing bone growth factors loaded bone grafts
a. Synthesis technique of bone growth factor loaded non-thermal treatment bone grafts
b. Controlled biodegradation technique of bone grafts for sustained release
2. Research and development informs and results
■ Manufacturing process of bone grafts and optimization
a. Establishment of non-thermal treatment bone grafts manufacturing process
○ Manufacturing process establishment for dispersing bone morphology proteins and drugs
○ Properties evaluation accordance with containing bone morphology proteins and drugs
○ Controlled release behaviors of bone morphology proteins and drugs
■ In vitro cell tests
○ Differentiation to osteoblast cell using boen marrow stromal cell-line
- Mineralized bone-like tissue observation; Alizarin red staining
■ In vivo animal test
○ For the animal test in rat calvarial defect model, bone graft granules were prepared from calcium phosphate bone cements. At 2 and 8 weeks post-surgery, the defect sites were filled with thin loose connective tissue including minimal new bone formation at the defect margins.
Consequently, the new bone substitute material, calcium phosphate bone cements wascbiocompatible and bioresorbable and promoted new bone formation. Inaddition, calcium phosphate bone cements granules have significant potential as bone graft material for bone regeneration.

목차 Contents

• 표지 ... 1
• 2. 제출문 ... 3
• 3. 보고서 요약서 ... 4
• 4. 요약문(한글) ... 5
• 5. 요약문(영문) ... 6
• I. 총괄현황 ... 7
• 6. 연구 성과 실적 및 향후 계획 ... 8
• 7. 참여연구원 현황표 ... 13
• II. 총괄연구과제 연구결과 ... 14
• 1. 연구개발과제의 배경 및 필요성 ... 15
• 2. 국내외 기술개발 현황 ... 20
• 3. 연구개발과제의 추진체계 ... 21
• 4. 연구개발수행 내용 및 결과 ... 22
• 5. 목표달성도 및 관련분야 기여도 ... 29
• 6. 향후 연구성과 추진 계획(*해당되는 경우만 작성) ... 29
• 7. 연구개발결과의 파급효과 ... 30
• 8. 연구개발결과의 활용계획 ... 31
• 9. 연구개발과정에서 수집한 해외과학기술정보 ... 31
• 10. 참고문헌 ... 31
• IV. 첨부서류 ... 32
• 1. 자체평가의견서 ... 34
• 2. 실적 증빙자료 ... 37
• 끝페이지 ... 48