초록

DNA 검출용 분석 시스템
1. 현장현시측정 (POCT)가 가능한 DNA 검출용 분석 시스템 기술 개발
가. 랩온어칩(Lab-on-a-chip) : 유전자 증폭이 가능하고 형광검출이 가능한 칩 설계
나. DNA 검출용 분석 시스템 : 유전자 증폭이 가능한 온도제어 모률, 증폭된 유전자의 검출을 위한 광학 모듈 개발
2. 가금류 질병 Target 유전자 마커
가. 조류인플루엔자 바이러스(Avian influenza virus - comman, H5, H7, H9) 검출이 가능한 유전자 마커 확보

Abstract ▼

Goal of this research was to develop the Point-of-Care Testing (POCT) system to detect molecular markers of Avian influenza virus. This virus has been known so dangerous even to human as well as fowl. More than 100 people and millions of fowl in various parts of the world have been killed and are st

Goal of this research was to develop the Point-of-Care Testing (POCT) system to detect molecular markers of Avian influenza virus. This virus has been known so dangerous even to human as well as fowl. More than 100 people and millions of fowl in various parts of the world have been killed and are still threatened by the Avian influenza virus. Therefore, we initiated this research for the development of rapid POCT diagnostic system using polymerase chain reaction (PCR). This method can amplify the signal sensitivity more than million times within 1 hours. We applied this powerful technology into the POCT system and improved amplification efficiency by reducing the amplification time. In addition, we also significantly improved the amplification efficiency and specificity by the application of the smart primer designing to detect the avian influenza viral genes. We believe that our primers and POCT system are useful for the early detection of avian influenza viral genes from the infected fowl as well as human blood samples. We hope our efficient POCT system can influence the current diagnostic market by replacing the old diagnostic system of rapid assay kit with low sensitivity.

목차 Contents

• 제출문...1
• 요약문...2
• SUMMARY...5
• 목차...7
• 제 1 장 서론...8
• 제 1 절 연구개발의 목적...8
• 제 2 절 연구개발의 필요성...8
• 제 3 절 연구개발의 범위...11
• 제 2 장 국내외 기술개발 현황...12
• 제 1 절 국외 기술개발 현황...12
• 제 2 절 국내 기술개발 현황...13
• 제 3 장 연구개발수행 내용 및 결과...14
• 제 1 절 현장현시측정 (POCT)이 가능한 DNA 검출용 분석 시스템 기술 개발...14
• 제 2 절 가금류 질병(조류인플루엔자, Avian Influenza virus) Target 유전자 마커...27
• 제 4 장 연구개발목표 달성도 및 대외기여도...40
• 제 5 장 연구개발결과의 활용계획...41
• 제 6 장 참고문헌...42