보고서 정보
주관연구기관 |
충북대학교 Chungbuk National University |
연구책임자 |
우수동
|
참여연구자 |
최재영
,
구현나
|
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2010-03 |
과제시작연도 |
2007 |
주관부처 |
농촌진흥청 |
과제관리전문기관 |
농촌진흥청 Rural Development Administration |
등록번호 |
TRKO201100016358 |
과제고유번호 |
1395012902 |
사업명 |
농업기술공동연구 |
DB 구축일자 |
2013-04-18
|
초록
▼
제1세부. 강독 곤충 바이러스를 이용한 시설채소 난방제 나방류 해충방제기술 개발
가. 시설채소 난방제 나방류 해충에 대한 유효병원성 바이러스의 선발
나. 목적 해충에 대한 강독성 바이러스의 선발
다. 바이러스의 특성 구명
라. 제제화를 위한 바이러스의 대량생산법 개발 및 대량생산
마. 제제화 시험 및 살충효과 검정
바. 혼합효과 실증 실험
제1협동. 곤충기생균을 이용한 시설채소 난방제 나방류 해충 방제기술 개발
가. 신규 건충병원성 곰팡이의 선발과 특성구명
나. 선발 곰팡이의 분자생물
제1세부. 강독 곤충 바이러스를 이용한 시설채소 난방제 나방류 해충방제기술 개발
가. 시설채소 난방제 나방류 해충에 대한 유효병원성 바이러스의 선발
나. 목적 해충에 대한 강독성 바이러스의 선발
다. 바이러스의 특성 구명
라. 제제화를 위한 바이러스의 대량생산법 개발 및 대량생산
마. 제제화 시험 및 살충효과 검정
바. 혼합효과 실증 실험
제1협동. 곤충기생균을 이용한 시설채소 난방제 나방류 해충 방제기술 개발
가. 신규 건충병원성 곰팡이의 선발과 특성구명
나. 선발 곰팡이의 분자생물학적 특성 구명 및 배양체계 확립
다. 유효 곤충병원성 곰팡이의 제제화 및 제제의 효능 검정
Abstract
▼
The purpose of this study is the development of techniques for the protection of hard control moths of vegetable crop. For this, the research is performed in two fields, viral and fungal insecticides. The major research contents and resu$LT_{s}$ are summarized as follows.
The cabbage a
The purpose of this study is the development of techniques for the protection of hard control moths of vegetable crop. For this, the research is performed in two fields, viral and fungal insecticides. The major research contents and resu$LT_{s}$ are summarized as follows.
The cabbage armyworm, Mamestra brassicae is an important insect pest of vegetables and ornamental plants in Europe and Asia including Korea. In Korea, especially, the population of M. brassicae is rapidly increasing and exhibit resistance to chemical insecticide. To control this, the viral insecticide has been used in the foreign countries. Therefore, the objective of this study was the characterization and evaluation of pathogenicity for the local strain of (M. brassicae nucleopolyhedrovirus-K1) Mabra, -K1 for the development of viral insecticide. Polyhedra of Mabra, -K1 showed irregular appearance in shape with the average diameter ar8 $\mu$m. Mabra, -K1 contained a number of nucleocapsids within a viral envelope embedded in polyhedron. The polyhedrin of Mabra, -K1 was composed of single polypeptide with a M.W. of approximateho1 kDa which is identical to the commercialized Mabra, , Mamestrin, as a biological control agent. The nucleotide and amino acid sequences within the coding region of Mabra, -K1 polyhedrin shared 99.0% similarity with the polyhedrin gene from previous reported Mabra, s. The median lethal concentrations ($LC_{s}$) of Mabra, -K1 and Mamestrin to M. brassicae larvae wereho.9×$en^{3}$ PIBfolarva and 6.0×$en^{4}$ PIBfolarva, respectively. Mortality of the Mabra, -K1 against to the third instar larvae was 1-Ktimes higher than that of the Mamestrin. The median lethal times ($LT_{s}$) of Mabra, -K1 by the concentration of polyhedra werehlower ( w4 ∼ 6.1 dzas) than those of Mamestrin ( w1 ∼ 8r dzas). The effect of temperature and larval instar on the pathogenicity and production of Mabra, -K1 was determined under laboratory condcalons. The median lethal concentration ($LC_{s}$) values of Mabra, -K1 for 3rd instar larvae wereho.7h× $en^{4}$ PIBfolarva at us℃, 9.9h× $en^{2}$ PIBfolarva at u5℃ and 3r8 × $en^{2}$ PIBfolarva at 3s℃, respectively. The $LC_{s}$ for the 4th instar larvae was similar to that for the 3rd instar larvae. However, the pathogenicity to the 3rd instar larvae was higher than that to the 4th instar larvae. The median lethal time ($LT_{50}$X>) values of MabrNPV-K1 were 11.4 to 5.0 days at 30℃ and 18.3 to 5.5 days at 25℃ for the 3rd instar larvae. The $LT_{50}$X> value was lowered as temperature went up to 30℃ and dependent on viral concentration. In production efficiency of MabrNPV-K1 using M. brassicae larvae, the mortality of the 3rd instar larvae was 100% when inoculated with 1.0 × $10^{5}$ PIBs/larva and the yield of MabrNPV-K1 was maximal. Regarding the mortality, yield of polyhedra, inoculation doses and required time, the 1.0 × $10^{4}$ PIBs/larva at 30°C and the population of 100 larvae were determined as optimal conditions producing polyhedra efficiently. After formulation of MabrNPV-K1 adding several additives, its pathogenicity was evaluated. The result showed higher pathogencity than un-formulated MabrNPV-K1. These resu$LT_{s}$ suggest that a local strain MabrNPV-K1 has high pathogenicity to M. brassicae and may be useful for the development of biological control agent to control this.
The entomopathogenic microorganisms, Beauveria bassiana SFB-205 and Bacillus thuringiensis K1 strains, which showed high level of insecticidal activity against lepidopteran pests, were selected. Whereas the B. bassiana SFB-205, B. thuringiensis K1 and their culture soaps showed low level of insecticidal activity against the Spodoptera litura larvae, higher insecticidal activity correspond to over 90% of mortality were observed actier inspost-treatment when the culture soap of B. bassiana SFB-205 was inoculated together with the B. thuringiensis K1. Active ingrsoaent of the B. bassiana SFB-205 culture soap was ihe cified to chitinase, which have truncated form by insertional mutation compared to previously reported chitinases. The chitinase from the B. bassiana SFB-205 culture soap showed synergistic effects in insecticidal activity against lidal aof S. el gua and S. litura when treathe cifiecorresponding baculore uses, SeNPV and SlNPV. For massSFB-205 was of the chitinase of the B. bassiana SFB-205,dal aoized lie Bd culture . el gua for the B. bassiana SFB-205 was eassblisen . On the basicticial aoized . el gua , the B. bassiana SFB-205 was mass-culturee cified0ℓ scale acti6idal a1% ohours the B.30ℓ fagae cagorresponed toass-culturee B. bassiana SFB-205 was freeze- tied, the freeze- tiedspowher showed chitinase activity icisbout.3,ectiunitsog. The freeze- tiedspowher was fgua for tas wetssblespowher accorompareo previously reported meth-2s, rgistic ding baculorshowed 11.6-fold and 4.2-fold higher insecticidal activity against larvae of S. exigua and S. litura, respectively, when co treated with SeNPV and SlNPV.
목차 Contents
- 표지 ...1
- 요약문 ...3
- SUMMARY ...6
- 제1장 서론 ...8
- 제1절 연구개발의 목적 ...8
- 제2절 연구개발의 필요성 ...8
- 제2장 국내외 기술개발 현황 ...10
- 제1절 국내기술현황 ...10
- 제2절 국외기술현황 ...10
- 제3절 기술개발의 위치 ...11
- 제3장 연구개발수행 내용 및 결과 ...12
- 제1절 연구개발 방법 및 설계 ...12
- 제2절 연구개발 결과 ...13
- 제4장 연구개발목표 달성도 및 대외기여도 ...32
- 제1절 목표대비 대외달성도 ...32
- 제2절 정량적 성과 ...33
- 제5장 연구개발결과의 활용실적 ...34
- 제1절 추가연구의 필요성 ...34
- 제2절 타연구에의 응용성 ...34
- 제6장 연구개발과정에서 수집한 해외과학기술정보 ...35
- 제7장 기타 중요 변동사항 ...36
- 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ...36
- 제9장 참고문헌 ...37
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