초록

1. 치과용 임플란트의 표면을 활성화하기 위한 플라즈마 방전조건 확립
2. 플라즈마 방전으로 개질된 표면의 물리적, 화학적 특성 분석
3. 활성된 표면에 대한 혈장 단백질 흡착 특성 등 생화학적 특성 분석
4. 동물모델 적용을 위한 활성된 표면에 대한 세포생물학적 기초 특성 분석
5. 염증 유발 유도성 일산화질소 합성효소 발현 확인 등을 통한 활성 표면의 안전성 검증
6. 동물모델 적용을 통한 활성표면의 유효성 검증
7. Gene expression 확인을 통한 활성 표면의 작용 기작 규명

Abstract ▼

1. The purpose and necessity of study
Modern clinical dental implants cannot be content with many demands, which is reducing time of surface modification by ultraviolet and medical expenses. Therfore, the purpose of this study is establishing surface activating technology by atmospheric pressure

1. The purpose and necessity of study
Modern clinical dental implants cannot be content with many demands, which is reducing time of surface modification by ultraviolet and medical expenses. Therfore, the purpose of this study is establishing surface activating technology by atmospheric pressure gaseous plasma discharge to heal periodontal diseases.
2. Results
(1) The DBD type plasma was treated with only helium gas and employed voltage were 2kV. It was most efficient condition to activate dental implant surfaces.
(2) The DBD type plasma treatment not only change the surface roughness, but also eliminate carbohydrate contaminants.
(3) We confirmed that the DBD type plasma treatment to titanium surface increases fibronectin adsorption.
(4) We found that a suitable condition to enhance spreading of osteoblast is appling pure helium gas. Voltage alteration does not affect to osteoblast spreading. Cell adhesion and proliferation were enhanced on DBD type plasma treated titanium surface for both MC3T3-E1 cells and hMSC cells.
(5) The DBD type plasma treated titanium surface does not induce inflammatory reaction compare to non-treated titanium surface.
(6) Actin cytoskeleton organization and focal adhesion formation were observed by phalloidin and anti-vinculin immunostaining. cells attached to the plasma treated samples were larger, had elongated shape and formed lamellipodia-like projections that were observed particularly at the ends of these actin filaments, in the elongated projections.
(7) Planar DBD type plasma treatment did not show significant effects in tibia defect animal model and it also couldn't affect osteoblast gene expression.
3. future plan
Changing dental implant surface to fresh by ultraviolet treatment takes long time. This method is hard to make finished goods and to provide in a reasonable price. According to the results, reducing the time required process and enhancing effectiveness of surface modification allow dental implant to increase the productivity, cut down the expenses and contribute improving quality of life.

목차 Contents

• 1. 겉표지 (앞면) ... 1
• 2. 제출문 ... 3
• 3. 보고서 요약서 ... 4
• 4. 요약문 (한글) ... 5
• 5. 요약문 (영문) ... 7
• 6. 연구 성과 실적 및 향후 계획 ... 8
• 6.1 총괄연구개발과제의 연구성과 실적 및 향후 계획 ... 8
• 6.2 연구성과 유형별 세부 내역 ... 9
• 7. 참여연구원 현황표 ... 12
• II. 총괄연구과제 연구결과 ... 13
• 1. 연구개발과제의 배경 및 필요성 ... 14
• 2. 국내외 기술개발 현황 ... 16
• 3. 연구개발과제의 추진체계 ... 16
• 4. 연구개발수행 내용 및 결과 ... 18
• 5. 목표달성도 및 관련분야 기여도 ... 36
• 6. 향후 연구성과 추진 계획 ... 37
• 7. 연구개발결과의 파급효과 ... 37
• 8. 연구개발결과의 활용계획 ... 38
• 9. 연구개발과정에서 수집한 해외과학기술정보 ... 39
• 10. 참고문헌 ... 39
• IV. 첨부서류 ... 42
• 1. 자체평가의견서 ... 44
• 2. 실적 증빙자료 ... 48