초록

1차년도에는 가축분뇨 퇴비 및 액비 종류별 유해미생물 및 병원성 기생충 조사를 주로 시행하였다. 그 내용으로 퇴비 종류에 따른 유해미생물 분리 동정, 액비 종류에 따른 유해미생물 조사 및 축산분뇨에 존재하는 병원성 기생충 분포를 조사하였다. 2차년도에는 퇴비화 과정 중 조건별 총균, 유해세균 및 병원성 기생충 변화를 조사하였고, 3차년도에는 가축분뇨 퇴비 및 액비 사용 농가의 유해미생물 및 병원성 기생충의 오염 실태 조사와 저감화 대책에 대하여 연구하였다.

Abstract ▼

The major objective of this study was to investigate the survival of indicator microorganisms and pathogens in livestock manure compost and liquid manure produced in Korea. A total of 110 livestock manure compost and 56 liquid manure samples were collected from manure company and pig farms. The samp

The major objective of this study was to investigate the survival of indicator microorganisms and pathogens in livestock manure compost and liquid manure produced in Korea. A total of 110 livestock manure compost and 56 liquid manure samples were collected from manure company and pig farms. The samples were examined to assess levels of total aerobic bacteria, coliforms, Escherichia coli, Salmonella spp., Escherichia coli O157:H7, Staphlyococcus aureus, Listeria monocytogenes, Cronobacter sakazakii, and Bacillus cereus. All samples were examined according to a standardized protocol and suspected colonies were confirmed by biochemical tests and Real Time PCR. In livestock manure compost, aerobic bacteria counts and coliforms ranged from 5.09 to 9.93 and 1.43 to 6.77 logCFU/g, respectively. E.coli was detected in 4.54%. In pathogens, C. sakazakii and B. cereus were isolated from livestock manure compost. In liquid manure, aerobic bacteria counts and coliforms ranged from 2.74 to 8.54 and 1.00 to 4.78 logCFU/mL, respectively. E.coli was detected in 14.28%. In pathogens, B.cereus were isolated from liquid manure. The results of this study could help to formulate microbiological guidelines for the use of organic manure in organic crop production.
The overall positive rates of intestinal parasites among pigs and beef cattle were 73.5% and 4.8%, respectively, and the double-infection rate was 10.3% in pigs. Of 136 specimens from pigs, Balantidium coli, Ascaris suum, and Entamoeba spp. infections were found in 88 (64.7%), 24 (17.6%), and 5 cases (3.7%), respectively. Of 105 beef cattle, Entamoeba spp. infections were detected in 5 cases (4.8%). From these results, it is shown that pigs raised on rural farms in Chungcheongnam-do had a high B. coli infection rate and a moderate A. suum infection rate. These results demonstrate that environmentally resistant cysts or eggs could be widespread on the farms examined, and thus an effective hygienic management system is needed to prevent them from serving as the source of infection for human beings.

목차 Contents

• 표 지 ... 1
• 제 출 문 ... 2
• 요 약 문 ... 4
• S U M M A R Y ... 8
• 제1장 서 론 ... 9
• 제1절 연구의 필요성 ... 9
• 제2장 국내외 기술개발 현황 ... 11
• 제1절 연구개발대상 기술의 국내 현황 ... 11
• 제2절 연구개발대상 기술의 국외 현황 ... 11
• 제3절 연구개발대상 기술의 국내 • 외 현황 비교 ... 11
• 제3장 연구개발수행 내용 및 결과 ... 13
• 제1절 연구재료 및 방법 ... 13
• 제2절 연구 결과 ... 30
• 제4장 연구개발목표 달성도 및 대외기여도 ... 205
• 제1절 목표대비 달성도 ... 205
• 제2절 정량적 성과 ... 205
• 제5장 연구개발결과의 활용계획 ... 210
• 제1절 정책적 측면 ... 210
• 제2절 기술적 측면 ... 210
• 제3절 경제적 • 산업적 측면 ... 210
• 제6장 중요 변동사항 ... 211
• 제7장 참고문헌 ... 212