[보고서]국내 농산물 유래 항노화 소재의 임상효과 평가 및 식의약소재 개발 융복합연구

천명훈

보고서 정보

주관연구기관

가톨릭대학교 산학협력단

연구책임자

천명훈

참여연구자

박용일 , 임동석

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2012-02

과제시작연도

2011

주관부처

농촌진흥청

사업 관리 기관

농촌진흥청
Rural Development Administration

등록번호

TRKO201200009929

과제고유번호

1395021397

DB 구축일자

2013-04-18

표 Expected value decrease of domestic agriculatural product after Korea-US FTA
표 Estimated budget for agricultural product import after Korea-US FTA
표 Expected trend of proportion change of population aged>65
표 Increase of medical expenses
표 Comparison of mortality by cause of death between 1997 and 2007
표 Status of Korea in the functional health food market (Nutrition Business Journal, 2006)
표 Popularity trend of functional health food in Japan
표 website: http://www.pl.barc.usda.gov/index.cfm
표 Reserach institution and subject in Europe
표 Expansion of functional health food market
표 Application for patents of major applicants.
표 Trend of functional health food patent application for elderly
표 Current status of functional health food for elderly in the technology lifecycle
표 Chemicla structures of anthocyanins in black soybean.
표 Manufacture process of anthocyanin and sample of capsule.
표 Purification of cyanindin-3-glucoside.
표 Mass fragmentation patterns of cyanidin-3-glucoside.
표 Dose dependent cytotoxity of anthocyanins
표 Effects of anthocyanins on LPS-induced nitric oxide production in RAW264.7 cells.
표 Inhibition of NF-kB by cyanidin-3-glucoside.
표 Effect of cyanidin-3-glucoside in immuno blotting and mRNA.
표 Time course DPPH radical scavenging by cyanindin-3-glucoside(left), trolox(middle) and psedo-first-order rate constant(k1) dependence on antiradical concentration.
표 Diagram of supercritical carbon dioxide purification system.
표 Experimental design of Supercritical carbon dioxide extraction conditions.
표 Experimental data on extract under different condition based on central composite design by response surface analysis
표 Polynominal equations calculated by RSM program for Supercritical carbon dioxide extraction conditions of sample
표 Predicted levels of extraction conditions of sample for the maximum reponses of variables by the ridge analysis.
표 Response surface for total Dp3g and Cy3g contents (ug/mg) of extractiontemperature and pressure.
표 Response surface for total Pt3g and Pn3g contents (ug/mg) of extraction temperatureand pressure
표 Effect of H2O2 on cell viability by MTT assay. The SK-N-SH cells were incubatedin fresh RPMI1640 with H2O2, from 1 to 50 μM, for 6 h (A). The cells preincubatedwith anthocyanin at the indicated concentration for 2 h and the medium includinganthocyanin was then removed and replaced with medium with or without 25 μM H2O2at 6 h (B). Results are from three experiments and expressed as the mean ± SD(n=4~8). A: ***<0.001 compared to control. B: ***<0.001 compared to 25 μM H2O2.
표 Suppression of H2O2-induced ROS formation by anthocyainin.Cells were pre-treated with indicated doses of anthocyanin for 2 hand then treated with 25 μM H2O2 for 6 h. The intracellular freeradical production was determined by DCF-DA methods
표 Effect of anthocyanin on HO-1 mRNA and protein expression in H2O2-stressed SK-N-SH cells.
표 Effect of H2O2 on Neu1, ST3GAL3, ST3GAL4 and ST3GAL6 mRNAexpression in SK-N-SH cells. The SK-N-SH cells were incubated infresh RPMI1640 with H2O2 from 1 to 25 M for 6 μ h. The quantity of Neu1,ST3GAL3, ST3GAL4 and ST3GAL6 mRNA in each sample was normalizedto the quantity of 18S rRNA (18S).
표 Effect of anthocyanin and H2O2 on Neu1, ST3GAL3, ST3GAL4 and ST3GAL6mRNA expression in SK-N-SH cells. The SK-N-SH cells were incubated in freshRPMI1640 with anthocyanin from 1 to 25 μM for 2 h. The anthocyanin containingmedia was removed and replaced with medium containing 25 μM H2O2. The cells wereexposed to 25 μM H2O2 for 6 h. The quantity of Neu1, ST3GAL3, ST3GAL4 andST3GAL6 mRNA in each sample was normalized to the quantity of 18S rRNA (18S).
표 Effect of anthocyanin on H2O2-induced phosphorylation of JNK and p38 MAP kinase inSK-N-SH cells. Results are from three experiments and expressed as the mean ±SD (n=3).
표 Fig. 3-1-18
표 Fig. 3-1-19
표 Fig. 3-1-20
표 PC12 세포에서 검정콩 anthocyanin의 세포내 [Ca2+]i 증가 억제를 통한 신경세포 사멸 억제 효과.
표 Anthocyanin inhibits glutamate-induced cell death in cultured rat hippocampal neurons.
표 Glutamate-induced [Zn2+]i and [Ca2+]i increase in cultured rat hippocampal neurons
표 Oxidant-induced [Zn2+]i and [Ca2+]i increase in cultured rat hippocampal neurons.
표 Glutamate-induced [Zn2+]i are regulated by redox state in cultured rat hippocampal neurons.
표 Anthocyanin inhibits glutamate-induced [Zn2+]i and [Ca2+]i increase in cultured rat hippocampalneurons
표 Survival of DU-145 prostate cell line by anthocianin concentration and time
표 AR and PSA presentation by anthocianin concentration and time of DU-145 prostate cell line
표 Change of tumor growth rate after anthocyanin administrationin prostate cancer xenoplatation model
표 Change of prostate weight in BPH mousemodel and control
표 Histopathologic findings in control (A) and BPH mouse (B)
표 Change of prostate weight in BPH mouse model by anthocyanin tratment
표 Histopathologic findings in BPH mouse afteranthocyanin tratment
표 (Left). Change of cell death in BPH mouse model by anthocyanin tratment
표 (Right). TUNEL stain in BPH mouse after anthocyanin tratment
표 Change of testis weight in varicocele mouse model
표 Histopathologic findings in control (A) and varicocele mouse (B)
표 (Left). Change of testis weight in varicocele mouse model by anthocyanin tratment
표 (Right). Change of germinal cell layer thickness in varicocele mouse model by anthocyanin tratment
표 Histopathologic findings by anthocyanin tratment. normal normal
표 TUNEL stain by anthocyanin tratment. normal (A), varicocelemouse (B), 4-week anthocyanin treatment (C), 8-weekanthocyanin treatment (D)
표 Annexin V analysis in chronic oxidative stress model for measurement of protectiveeffect of anthocyanin
표 TUNEL stain in chronic oxidative stress model by anthocyanin treatment
표 Cahnge of ROS in chronic oxidative stress model by anthocyanin treatment
표 Cellular appearance in chronic oxidative stress model by anthocyanintreatment
표 Change of proteom profiling in chronic oxidative stress model byanthocyanin treatment
표 Change of BAX, BAD, Caspase 3 protein in chronic oxidativestress model by anthocyanin treatment
표 Change of Bcl2 protein (red color) in chronic oxidative stress model byanthocyanin treatment
표 Cataract Ex vivo model. (A) control (B) 100 uM H2O2 1-wk treatment (C)100 uM H2O2 2-wk treatment
표 Change of lens turbidity after treatment of blackbean anthocyanin in pig
표 cornea neovascularization model
표 Inhibitory effect of anthocyanin on neovascularization
표 VEGF expression after anthocyanin treatment
표 Trend of retinal degeneration by MNU concentration and time after treatment.H&E staining
표 ERG analysis of retinal degeneration by MNU concentration and time after treatment
표 Protective effect of anthocyanin on retinal cells. Difference of structural and functionalcharacteristics of retina by time flow. Left: H&E staining. Right : ERG recording
표 Anti-inflammatory effect of balckbean anthocyanin. GFAP Immunohistochemistry.
표 Neuroprotective effect of balckbean anthocyanin. Evaluation of excitatory mechanism.
표 DCF-DA stain to evaluate antioxidative effect of balckbean anthocyanin.
표 Evaluation of neuroprotective effect of balckbean anthocyanin. (Mitochodrial membrane voltage)
표 Neuroprotective effect of balckbean anthocyanin (C3G) in OGD model
표 Evaluation of neuroprotective effect of balckbean anthocyanin.
표 DCF-DA stain to evaluate antioxidative effect of balckbean anthocyanin (C3G)
표 C3G plasma concentration after administrationof balckbean crude extract 500 mg
표 Antioxidative capacity after administration ofbalckbean crude extract 500 mg
표 CYTOTOXIC EFFECT OF ANTHOCYANINS ON rbl-2H3 cells. The date are themeans ±SD (n=4)
표 Effects of anthocyanins on antigen-induce intracellular ROS production (A) inRBL-2H3 cells and DPPH radical scavenger activity (B). the date are the means±SD (n=4). *p<0.05 vs. positive control (DNP-BSA); **p0.01 vs. positivecontrol(IgE+DNP-HSA)
표 Effect of anthocyanins on -hexosaminidase β release in IgE-antigencomplex-stimilated RBL-2H3 cells. the date are the means±SD (n=4).
표 Effect of anthocyanins on RNA expression of the IL-4, IL-13 and TNF- . The α data are the means±SD(n=3). *p<0.05;**p<0.01 vs. positive control (IgE+DNP-HSA). The amount of IL-4. IL-13 and TNF-αMRNA in each sample was normalized to the amount of GAPDH. The "fold-induction" of correspondingmean value in control cells.
표 (Left) The effect of Anthocyanins on the viability of the MC3T3-E1 as determined byMTT assay
표 (Right) Effect of Anthocyanin on MC3T3-E1 differentiation and mineralization. ALPwas visualized by ALP staining at differentiation day 5.
표 Anthocyanin effect on cell death by OGD (A, B). U87 cell death by OGD and theanthocyanin effect against it (C, D); anthocyanin effect on the accumulation ofROS by OGD
표 Anthocyanin effect on autophagy induction by OGD (A).Anthocyanin effect on LC3-Ⅱconversion of OGD (B).
표 Inhibition of ATG5 expression by ATG5siRNA (A). ATG5 siRNA effecton cell survival increased by anthocyanin (B). rapamycin effect on cellcount decrease by OGD ?
표 Induction of autophagy by anthocyanin. LC3 formation after anthocyanin treatment onU2OS cell (A). Conversion of LC3 I to LC3 II in the same condition as (A) (B)
표 ( Left) Cell proliferation of 3T3-L1 cell
표 (Middle) Cell viability
표 (Right) TG accumulation
표 (Left) Glycerol-3-phosphate dehydrogenase (GAPDH)
표 (Middle) Lipolysis
표 (Right) PPARγ expression
표 LPS- induced nitrite production
표 Co- culture nitrite production
표 Co- culture MCP - 1 expression
표 Co- culture IL- 6 expression
표 .Co- culture TNF - expression
표 Fig. 3-1-87. Feeding schedule of crop extracts
표 Fig. 3-1-88. The antiviral effects for Ad36 in crops extracts
표 The Ad36-induced obesity reduction effects of crops extracts
표 The size and weight of reproductive fat pads reduction effectsby crops extracts
표 The pro-inflammatory cytokines reduction effects in reproductive fat pads by crops extracts
표 The reduction effects of the infiltration of immune cells by crops extracts
표 The reduction effects of the infiltration of macrophages by crops extracts
표 Acorn extract manufacturing
표 Acorn extract yield
표 characteristics of acorn extract by extract condition
표 solubility of acorn extract by the solvents
표 Isolation of acetylcholinesterase inhibiting material from acorn extract
표 HPLC (A) and LC-MS-MS (B) results of inhibitory material I
표 HPLC (A) and LC-MS-MS (B) results of inhibitory material II(C2) and III(C3)
표 Chemical structures of inhibitory materials
표 Acetylcholinesterase inhibition of the inhibitory materials
표 Effect of AEx from Quercus acutissimaCARR, on LPS-induced nitrites production.
표 Effect of AEx from Quercus acutissimaCARR, on LPS-induced IL-1β production
표 Fractionation of ethanol extract (AEx)
$Effect of AEx and Acetone-Fractions on LPS-induced COX-2/iNOS mRNA production.RAW 264.7 cell were stimulated with LPS(1ug/ml) for 30 min, followed by treatment withindicated concentrations of AEx and acetone-fractions. The mRNA levels of COX-2/iNOSware determined by RT-PCR analysis. GAPDH was used as internal control.$ 표 Effect of AEx and Acetone-Fractions on LPS-induced COX-2/iNOS mRNA production.RAW 264.7 cell were stimulated with LPS(1ug/ml) for 30 min, followed by treatment withindicated concentrations of AEx and acetone-fractions. The mRNA levels of COX-2/iNOSware determined by RT-PCR analysis. GAPDH was used as internal control.
$HPLC analysis for existence of gallic acid and tannic acidin the AEx and F2 fraction$ 표 HPLC analysis for existence of gallic acid and tannic acidin the AEx and F2 fraction
표 Acorn extract inhibits glutamate-induced neural cell death in cultured rat hippocampalneurons
표 Glutamate-induced [Zn2+]i and [Ca2+]i increase in cultured rat hippocampal neurons
표 Oxidant-induced [Zn2+]i and [Ca2+]i increase in cultured rat hippocampal neurons.
표 Glutamate-induced [Zn2+]i are regulated by redox state in cultured rat hippocampal neurons.
표 Fig. 3-2-21
표 MTT analysis evaluating the influence of acorn extract on apoptosis
표 Evaluation of lens epithelium migration and proliferation effect of acorn extract
표 Retinal protection of acorn extract
표 Establishment of seizure model using pilocarpine (A-C) control (D-F) seizure model
표 seizure model using culture of hippocampal slice
표 Evaluation of acorn extract on hippocampal cell in epileptic condition. Cresyl violet stain
표 Visual predicted checks using observed and simulated acetylcholinesterase (AChE) activity.Circles Observed AChE activity data (open no-treatment period, red acorn extract, bluegalantamine); these AChE activity symbols were jittered to avoid overlapping betweentreatment and no-treatment periods. Black lines Median (solid) and 75% confidence interval(CI; broken) of simulated AChE activity for the notreatment period. Colored lines median(solid) and 75% CI (broken) of simulated AChE activity with the respective treatment (redacorn extract, blue galantamine). MESOR Midline estimating the statistic of rhythm
표 Statistical maps showing the regions of cortical thickness correlated with HAM-D17 totalscore (a), CERAD-K word list recall score (b), and Stroop Word-Color Interference testscore (c) in the LOD group (po0.05 FDR corrected). Maps are shown for right and lefthemispheres in lateral and medial views respectively, and significant regions are shown inblue (negative correlation) and red (positive correlation). LOD, late-onset depression;CERAD-K, Korean version of Consortium to Establish a Registry for Alzheimer’
표 Composition of acorn jelly powder added biscuit
표 Composition of acorn extract added biscuit
표 Solidity of acorn jelly powder added biscuit dough
표 Solidity of acorn jelly powder added biscuit
표 Solidity of acorn extract added biscuit dough
표 chromaticity of acorn jelly powder added biscuit dough (Hunter
표 chromaticity of acorn jelly powder added biscuit (Hunter
표 chromaticity of acorn extract added biscuit dough (Hunter
표 chromaticity of acorn extract added biscuit (Hunter
표 spread factor of acorn jelly powder added biscuit dough (Hunter
표 spread factor of acorn jelly powder added biscuit (Hunter
표 Characteristics test of acorn jelly powder added biscuit
표 Characteristics test of acorn extract added biscuit
표 microbiologic change of acorn jelly powder added biscuit and acorn extract addedbiscuit at -4℃ and at room temperature
표 Composition of acorn jelly powder added muffin
표 Appearnce of acorn jelly powder and acorn extract added muffin
표 Moisture contents of acorn jelly powder and acorn extract added muffin
표 Weight reduction of acorn jelly powder and acorn extract added muffin
표 chromaticity of acorn jelly powder and acorn extract added muffin (Hunter
표 adhesiveness of acorn jelly powder and acorn extract added muffin
표 Characteristics test of acorn jelly powder and acorn extract added muffin
표 Mouse model of airway inflammation induced by ovalbumin (OVA) and treatment withacorn ethanol extract (AEx) or tannic acid. i.p., intraperitoneal.
표 Effects of acorn ethanol extract (AEx) on IL-4, IL-13, and TNF-α production in thebronchoalveolar lavage fluid (BALF) of OVA-induced asthma mice. Mice were challenged with 2%OVA for 10 min and AEx or tannic acid was orally administered every day from day 13 to 27. Onday 27, mice were sacrificed for BALF as described in Materials and Methods. Normal, mice treatedwith PBS only; OVA, OVA-sensitized/challenged mice; Tannic acid (75 mg/kg body weight) +OVA-sensitized/challenged mice; AEx, acorn ethanol extract (100 or 300 mg/kg body weight) +OVA-sensitized/challenged mice. The protein levels of (A) IL-4, (B) IL-13, and (C) TNF-α wereassayed by ELISA. The data are the means ± SD (n = 6-12). **P<0.01;***P<0.001 vs.OVA-sensitized control asthma group.
표 Effects of acorn ethanol extract (AEx) on IL-4 and IL-13 mRNA expression andprotein production in the lung tissues of OVA-induced asthma mice. On day 27, mice weresacrificed for lung tissues as described in Materials and Methods. Normal, mice treated with PBS only;OVA, OVA-sensitized/challenged mice; Tannic acid (75 mg/kg body weight) +OVA-sensitized/challenged mice; AEx, acorn ethanol extract (100 or 300 mg/kg body weight.) +OVA-sensitized/challenged mice. The mRNA and protein levels of (A, C, E) IL-4 and (B, D, F) IL-13were assayed by RT-PCR and ELISA, respectively. The IL-4 and IL-13 mRNA levels in each samplewere normalized to the -actin levels. The density of each mRNA band was β quantified using KodakImage Station (Kodak, NY, USA). The data are the means ± SD (n=6-12). *P<0.05; **P<0.01;***P<0.001 vs. OVA-sensitized control asthma group
표 Effects of acorn ethanol extract (AEx) on PPAR- γ, INFγ, IL-5 and TNF-α mRNAexpression in the lung tissues of OVA-induced asthma mice. Mice were sensitized, challenged, andadministered with AEx or tannic acid as described in the Text. On day 27, mice were sacrificed for lungtissues as described in Materials and Methods. Normal, mice treated with PBS only; OVA,OVA-sensitized/challenged mice; Tannic acid (75 mg/kg body weight) + OVA-sensitized/challenged mice;AEx, acorn ethanol extract (100 or 300 mg/kg body weight.) + OVA-sensitized/challenged mice. ThemRNA levels of PPARγ, INFγ, IL-5 and TNF-α were assayed by RT-PCR. The mRNA levels in eachsample were normalized to the β-actin levels. The density of each mRNA band was quantified usingKodak Image Station (Kodak, NY, USA). The data are the means ± SD (n=6-12). *P<0.05; **P<0.01;***P<0.001 vs. OVA-sensitized control asthma group.
표 Effect of acorn ethanol extract (AEx) on serum cytokines levels in a mouse modelof asthma. The cytokine mRNA levels in each sample were normalized to the β-actin levels
표 Effects of acorn ethanol extract (AEx) on body weight change, serum totalcholesterol, triglyceride, GPT and GOT levels in OVA-induced asthma mice. Mice were sensitized,challenged, and administered with AEx or tannic acid. On day 27, mice were sacrificed for serum asdescribed in Materials and methods. Body weights were weighed prior to sacrifice. Normal, mice treatedwith PBS only; OVA, OVA-sensitized/challenged mice; Tannic acid (75 mg/kg body weight) +OVA-sensitized/challenged mice; AEx, acorn ethanol extract (100 or 300 mg/kg body weight.) +OVA-sensitized/challenged mice. Sera were prepared and assayed for total cholesterol, triglyceride, GPT andGOT levels as described in the text. The data are the means ± SD (n = 6 ~ 12). *P<0.05; **P<0.01;***P<0.001 vs OVA-sensitized control asthma group.
표 Comparison of antioxidative capacity of mulberry
표 Procedures for the preparation of Mulberry fibers from the water extracts of KoreanMulberry.
표 Yield of JS-MP-1 purification
표 Fractionation of crude polysaccharide by DEAE-cellulose column chromatography
표 Monosaccharide composition analysis of the purified Mulberry polysacharide by HPAEC-PAD after TFA fydrolysis. (A) standard. (B) acid hydrolysis from purified Mulberry polysaccharide.
표 Chemical cpmposition of the purified Mulberry polysacharide JS-MP-1
표 Estimation of the molecular weight of JS-MP-1 by size exclusion HPLC. The standard size makers were dextran (sigma): 2000(a), 464..336(b), 71,327(c), 43(d) kDa
표 FT-IR spectra of purofied Mulberry polysaccharide, JS-MP-1.
표 Elemental analysis of JS-MP-1
표 1H-NMR analysis of JS-MP-1
표 HMQC and COSY analysis of JS-MP-1
표 Cytotoxicities of JS-MP-1 in RAW264.7 cells. By assay.
표 Immunomodulating effect of JS-MP-1 in RAW264.7 cells. Cells were coincubated with the indicated doses of JS-MP-1 for 24 hr. As a control, commercial LPS (0.1μg/ml) was used. The level of TNF-α (A) and (B) in the suprematants for the cultures was determined by ELISAkit. The cells were treated with the mixture of LPS (0.1μg/ml) and different concentration (0.625, 1.25, 2.5, 10, and 50 μg/ml) of JS-MP-1
표 Effects of JS-MP-1 and LPS on the expreesion of COX-2 in Raw 264.7 cells. Cells (5X104 cells.ml) were incubated with JS-MP-1 (1, 5, 10, 50 g/μ ml) or LPS (0.1μg/ml) for24h
표 Effects of JS-MP-1 and LPS on the expreesion of iNOS in Raw 264.7 cells. Cells (5X 104cells.ml) were incubated with JS-MP-1 (1, 5, 10, 50 μg/ml) or LPS (0.1μg/ml) for 24h.
표 ICP and ICP/MAP ration in DM impotence model
표 Fig. 3-3-13.
표 Masson
표 erection-related factors after melberry fruit treatment in DM impotence model
표 TUNEL stain
표 Change in Akt, Bad, SOD expression
표 Annexin V anlysis for cellular protection by mulberry fruit extract in chronic oxidative stress lens model
표 lens epithelial cell migration and proliferation by mulberry fruit extract
표 ERG findings - Retinal protection of mulberry fruit extract
표 Histopathologic findings - Retinal protection of mulberry fruit extract
표 Anti-inflammatory effect of mulberry fruit extract GFAP Immunohistochemistry.
표 Neuroprotective effect of mulberry anthocyanin in OGD model
표 Neuroprotective effect of mulberry anthocyanin in OGD model
표 Neuroprotective effect of mulberry anthocyanin by stabilization of mitochondrial membrane voltage
표 Plasma C3G concentration after administration of mulberry fruit extract 500 mg
표 Antioxidative capacity after mulberry fruit extract 500 mg
표 lyophilization of mulberry fruit
표 Development of mulberry C3G-containing foods
표 The reduction effects of NFκB signaling by mulberry extracts
표 The antiviral efficacy of chemical composition in mulberry extracts
표 Improvement of cell survival by 70% ethanol extract againt oxidative stress
표 Inhibition of NO production
표 iNOS lowering effect
표 neuroprotecive effect
표 Neuronal cell density after extract administration
표 Acetylcholinesterase inhibition
표 Result of Passive avoidance performance test
표 Result of Morris water maze result
표 Evaluation of HE extract effect on hippocampal cell. Cresyl violet stain.
표 Evaluation of HE extract effect on hippocampal cell in epiletic condition (NeuN stain)
표 Change in the number of BrdU-positive cellls
표 BrdU-positive cellls in hippocampal dentate gyrus after HE extract treatment
표 BrdU-positive cellls in hippocampal dentate gyrus after BrdU administration. 2 hr (A) and 28 days (B)
표 BrdU-positive cellls in hippocampal dentate gyrus after HE extract treatment (NeuN stain)
표 tail suspension test resut for evaluation of anti-depressive effect
표 General composition of the goat yogurt and placebo provided for clinical test
표 Lactic acid bacterial counts of the goat yogurt provided for clinical test
표 IGF-1 content of the goat yogurt(blue) and milk(blank) provided for clinical test
표 Electropherogram of goat(upper) and cow(lower) milk
표 Profiles of as- caseins and b- caseins compositions of goat and cow milks determined by CE
표 MALDI-TOF analysis of goat(a) and cow milk(b)
표 Molecular weights of major proteins in goat and cow milk
표 Time schedule of food allergy model
표 Histamin production of the mast cell with lactic acid bacterial treatment
표 16S rRNA sequence of the selected lactic acid bacteria
표 Comparison of food allergy response after feeding lactic acid bacteria
표 Foxp3+ Treg cells in food allery model animals with lactic acid bacteria
표 The structure of macromolecule and SEM image
표 Nano particle containg blackbean anthocyanin
표 Size measurement of the nano particlecontaing blackbean anthocyanin
표 membrane potential measurement ofthe nano particle containg blackbeananthocyanin
표 Release control of the nanoparticle containg blackbeananthocyanin
표 Structural stability ofblackbean anthocyanin inthe nano particle
표 Improved DPPH radical scavenging ofthe nano particle containg blackbeananthocyanin
표 Improved inhibition on cellularoxidative stress of the nanoparticle containg blackbeananthocyanin
표 1H-NMR spectrum of acetylated ucoidan
표 Acetylated fucoidan의 critical micelle concentration measurement
표 mulberry anthocyanin and AF concentration measurement
표 mulberry anthocyanin and AF charge complex particle size measurement
표 shape analysis ofmulberry anthocyanin andcharge complex ofacetylated fucoidan
표 Improved cell viability of mulberryanthocyanin and charge complex ofacetylated fucoidan
표 The analysis of antioxidative activity for fucoidan-anthocyanin nano complex
표 The anti-tumor activity for fucoidan and fucoidan-anthocyanin nano complex
표 Constituents used to form multipore microparticle containing anthocyanin
표 FE-SEM image of the microparticle described in Tab.1
표 FE-SEM image of the microparticle described in Tab.2
표 Digital image of microparticle dispersed in PBS solution
표 Anthocyanin release from the microparticle
표 Manufacturing condition for starch containing microparticle
표 FE-SEM image and drug(형광:FITC 결합 약물) 분포
표 Tapped density.
표 anthocyanin release from starch containing microparticle
표 DPPH elimination by the sustained anthocyanin release from starch containing microparticle
표 Lung inhalation of multipore microparticle
표 Maintenance of micropore in lung
표 Histologic analysis of lung tissue by the microparticle treatment
표 담배연기 추출물을 폐세포 (A549)에 처리했을때 안토시안이 봉입된 다공성 미립구 혹은 free 안토시안이 존재할경우 세포독성 (n=7): 첫번째 bar:2일, 두번째 bar:3일, 세번째 bar:5일 경과후.
표 안토시아닌이 봉입된 다공성 미립구 제형을 통한 만성폐쇄성질환 예방및 치료 개요.
표 Findings from cornea alkali burn model after the topical eye drop administration
표 The extraction of sanghwang mushroom
표 Proanthocyanidin of grape seeds inhibits glutamate-induced neural cell death in cultured rathippocampal neurons by suppressing calcium signal and NO production.
표 Proanthocyanidin decreased glutamate- and 0.1 mM [Mg2+]o-induced NO formation
표 Glutamate-induced [Zn2+]i and [Ca2+]i increase in cultured rat hippocampal neurons
표 Oxidant-induced [Zn2+]i and [Ca2+]i increase in cultured rat hippocampal neurons
표 Glutamate-induced [Zn2+]i are regulated by redox state in cultured rat hippocampal neuron
표 GSPE protects cells against 0.1 mM [Mg2+]o?induced neurotoxicity
표 The measurement of edema
표 Relieving effect of Eucommia ulmoides Oliv on edema
표 Inhibitory effect of Eucommia ulmoides Oliv on rheumatoid factor expression
표 Inhibitory effect of Eucommia ulmoides Oliv on TNF-a, IL-1b, IL-6 expression
표 Inhibitory effect of Eucommia ulmoides Oliv on RANKL expression
표 Inhibitory effect of Eucommia ulmoides Oliv on pannus formation
표 Quantitative outcome from the research

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국내 농산물 유래 항노화 소재의 임상효과 평가 및 식의약소재 개발 융복합연구
Clinical evaluation of anti-aging materials from domestic agricultural products and convergent approaches for the development of food-medicinal materials 원문보기

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국내 농산물 유래 항노화 소재의 임상효과 평가 및 식의약소재 개발 융복합연구 Clinical evaluation of anti-aging materials from domestic agricultural products and convergent approaches for the development of food-medicinal materials 원문보기

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국내 농산물 유래 항노화 소재의 임상효과 평가 및 식의약소재 개발 융복합연구
Clinical evaluation of anti-aging materials from domestic agricultural products and convergent approaches for the development of food-medicinal materials 원문보기

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