초록

○ 기능이 검정된 SnRK2, MYB 등 기개발 유전자 분리 및 형질전환용 운반체 제작
○ Agro infilteration 등을 통한 transient transformation 방법 구축
○ 우수 벼 품종에 항충성 유전자 Cry1Ac1 형질전환, 무 선발마커 전환체로부터 PCR 및 Immunostrip로 형질전환체 선발

Abstract ▼

Virus-induced gene silencing (VIGS) technique, based on Post-Transcriptional Gene Silencing (PTGS) is a powerful tool to study rapid loss-of-function in genomics. In this study, transient transformation method was developed to assess stress tolerance among many stress-related genes. A stress toleran

Virus-induced gene silencing (VIGS) technique, based on Post-Transcriptional Gene Silencing (PTGS) is a powerful tool to study rapid loss-of-function in genomics. In this study, transient transformation method was developed to assess stress tolerance among many stress-related genes. A stress tolerant gene, NbOSAK was isolated from N. benthamiana and subcloned into pTRV2 vector using Gateway system as a positive control. Transient transformants was generated via leaf infiltration and visually confirmed by silencing phytoene desaturase gene as a marker gene. The system can be used for screening abiotic and biotic stress tolerance genes.
The generation of transgenic plants free of antibiotic resistance markers is a major challenge to plant biologists and plant breeders. Our transformation method is based on so-called ‘marker-free’ transformation. Marker-free transformation has been successfully demonstrated by the use of several plant and non-plant genes that are capable of promoting explant regeneration. The hybrid Bacillus thuringiensis (Bt) δ-endotoxin gene Cry1Ab/Ac was used to develop a transgenic Bt rice (Oryza sativa L.) targeting lepidopteran insects of rice. Calli initiated from the embryos of dehulled rice (Oryza sativa L. cv. Gopum) grains were transformed by pRDA-RTB containing rbcs promoter and Bt gene. We didn't confirm any transgenic plants using genomie PCR and BT strip test though we've got about 300 putative transgenic shoot group.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 4
• 제1장 서 론 ... 5
• 제2장 국내외 기술개발 현황 ... 5
• 제1절 국내 기술개발 현황 ... 5
• 제2절 국외 기술개발 ... 6
• 제3장 연구개발수행 내용 및 결과 ... 7
• 제 1 절 시험방법 ... 7
• 제 2 절 실험결과 ... 8
• 제4장 연구개발목표 달성도 및 대외기여도 ... 13
• 제1절 목표대비 달성도 ... 13
• 제2절 정량적 성과 ... 13
• 제5장 연구개발결과의 활용계획 ... 14
• 제6장 중요 변동사항 ... 14
• 제7장 참고문헌 ... 14
• [참고 1] 연차평가 지적사항 및 조치결과 ... 19
• [참고 2] 주요연구 성과 ... 20
• 유전자 기능 신속 검정을 위한 transient transformation 방법 구축 ... 20
• 마커프리 형질전환 기술을 이용한 GM벼 육종 소재 개발 ... 21
• 주요 결과 요약서 ... 22