[보고서]천연섬유고분자재료의 기능성식품화 기초연구 : 다양한 재생실크 (세리신함량별, 누에품종별) 및 히드록시에틸 메틸셀룰로오스(HEMC)의 제조, 구조/용액특성 및 기능성 식품으로서의 효능과의 관계 규명 연구

[국가R&D연구보고서] 천연섬유고분자재료의 기능성식품화 기초연구 : 다양한 재생실크 (세리신함량별, 누에품종별) 및 히드록시에틸 메틸셀룰로오스(HEMC)의 제조, 구조/용액특성 및 기능성 식품으로서의 효능과의 관계 규명 연구
The basic study on the application of natural polymeric material to functional food: the preparation of various regenerated silk and hydroxyethyl methylcellulose (HEMC) and the investigation on the relationship between their structural characteristics, solution properties and physiological effects for functional food application 원문보기

보고서 정보
주관연구기관	경북대학교 KyungPook National University
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2011-08
과제시작연도	2010
주관부처	교육과학기술부 Ministry of Education and Science Technology(MEST)
과제관리전문기관	한국연구재단 National Research Foundation of Korea
등록번호	TRKO201300012671
과제고유번호	1345127349
사업명	일반연구자지원
DB 구축일자	2013-08-26
키워드	재생실크.히드록시에틸 메틸셀룰로오스.천연섬유고분자.기능성식품.구조특성.용액특성.항산화효능.혈당.콜레스테롤.Regenerated silk.Hydroxyethyl methylcellulose.Natural polymer.Functional food.Structural characteristics.Solution properties.Preventive effect of chronic inflammatory disease.Blood glucose.Cholesterol.
DOI	https://doi.org/10.23000/TRKO201300012671

초록 ▼

연구의 목적 및 내용
본 연구에서는 재생실크(세리신함량별, 누에품종별)와 히드록시에틸 메틸셀룰로오스(HEMC)(점도별, 치환도별)의 제조, 구조/용액특성 및 기능성식품으로서의 효능을 명확히 규명하고 이들 인자들간의 관계를 규명함으로써 천연섬유소재를 향후 기능성식품 분야로 응용을 위한 기초연구자료 및 기초원천기술을 확보하는 것이 궁극적인 연구목표로 세부적인 내용은 다음과 같이 요약할 수 있음.
1. 세리신함량 및 누에품종이 다른 재생실크의 제조법 확립
2. 점도 및 치환도 함량이 다른 히드록시에틸 메틸셀룰로오스 (HEMC)의 정제법 확립
3. 재생실크와 HEMC의 구조특성 및 용액특성 규명
4. 다양한 재생실크의 in vivo 당뇨 및 콜레스테롤 저하활성 규명
5. HEMC의 in vivo 당뇨 및 콜레스테롤 저하활성 규명

연구결과
1. 세리신을 포함한 재생실크, 작잠실크의 제조법 확립
2. 식품용 HEMC 제조조건(정제조건) 확립
3. 세리신 포함 재생실크 및 HEMC의 구조특성, 유변물성, 용액특성 규명 완료
4. 세리신 포함한 재생실크의 혈당/콜레스테롤 저하 효능 규명
→ 모든 재생실크 샘플에서 혈당/콜레스테롤 저하 효능이 나타났고, 특히 세리신 함
량이 증가함에 따라 혈당/콜레스테롤 저하 효능이 크게 증가하는 것으로 나타남.
5. 작잠 실크의 혈당/콜레스테롤 저하 효능 규명
→ 작잠 실크도 혈당/콜레스테롤 저하 효능이 좋은 것으로 나타남.
6. HEMC의 혈당/콜레스테롤 저하 효능 규명
→ HEMC에서도 혈당/콜레스테롤 저하 효능이 좋은 것으로 나타났으며, 종래의 HPMC보다 우수하고, 특히 분자량(점도)가 증가할수록 효능이 크게 증가하는 것으로 나타남.

연구결과의 활용계획
o 본 연구과제에서 사용한 천연섬유고분자 재료인 1) 세리신 포함 실크, 2) 작잠실크, 3)HEMC 모두 우수한 생리활성을 나타냄을 확인함으로써, 이들 재료의 생리활성 효능 관련 원천기술을 확보하였음. 이를 바탕으로 현재까지 논문 4건(게재승인 1건 포함), 특허 1건 게재/출원하였고, 추가로 논문 3~4편 및 특허 1건을 게재/출원할 계획임.
o 특히, 본 과제를 통해 확보한 천연섬유고분자 재료들의 생리활성 관련 원천기술 및 원천특허를 활용하여 산업화를 위한 후속연구를 추진할 계획임.

Abstract ▼

Purpose&contents
In this research, various regenerated silk fibroins and hydroxyethyl methylcellulose was prepared and their structural characteristics, solution properties, and biological activities will be evaluated to obtain the fundamental technology for the applications to functional food area. Research contents are as following;
1. The preparation of regenerated B. mori silk containing sericin and A. periny silk.
2. The refinement of hydroxyethyl methylcellulose (HEMC) with various viscosity and substitution degrees.
3. The investigation of structural characteristics and solution properties of regenerated silks and HEMC
4. The investigation on the blood sugar and cholesterol lowering effect of various silks
5. The investigation on the blood sugar and cholesterol lowering effect of various HEMCs

Result
1. The establishment of the preparation method of regenerated silks
2. The establishment of refine condition of HEMC for food application
3. The investigation of structural characteristics and solution properties of regenerated silks and HEMC
4. The investigation on the blood sugar and cholesterol lowering effect of silk containing sericin
→ All silk sample showed good blood glucose and cholesterol lowering effects.
Especially, those effect increased signficantly as the sericin content increased.
5. The investigation on the blood sugar and cholesterol lowering effect of A. pernyi silk
→ A. pernyi silk also exhibited good blood glucose and cholesterol lowering effects.
6. The investigation on the blood sugar and cholesterol lowering effect of HEMC
→ HEMCs displayed good blood glucose and cholesterol lowering effects. They showed a better effect than hydroxypropyl methylcellulose (HPMC). The lowering effects became more evident as the molecular weight (viscosity level) of HEMC increased.

Expected Contribution
o Addition application of patent and publication in related journals
o A subsequent research using the basic technology obtained by present research project, will be tried in the future to apply those natural fiber materials to functional food industry.

목차 Contents

일반연구자지원사업 최종보고서(최종분) ... 1
목차 ... 2
연구계획 요약문 ... 3
연구 결과 요약문 ... 4
한글요약문 ... 4
SUMMARY ... 5
연구내용 및 결과 ... 6
1. 연구개발과제의 개요 ... 6
2. 국내외 기술개발 현황 ... 12
3. 연구수행 내용 및 결과 ... 15
4. 목표 달성도 및 관련 분야에의 기여도 ... 101
5. 연구결과의 활용계획 ... 102
6. 연구과정에서 수집한 해외과학기술정보 ... 102
7. 주관연구책임자(공동연구원) 대표적 연구 실적 ... 103
8. 참고 문헌 ... 103
9. 연구 성과 ... 104
10. 기타사항(중요 연구 변경사항 등) : 해당 사항 없음 ... 105
[별첨1] 대표연구성과 ... 106

표/그림 (100)

표 국내 기능성식품 시장규모
표 미국 기능성식품 시장규모 및 전망
표 2003년 약효군별 세계 의약품 매출 현황
표 Degumming ratio of silk cocoon degummed with various sodium oleate contents.
표 Residual sericin content of silk cocoon degummed with various sodium oleate contents.
표 Production yield of silk regeneration.
표 The molecular weight distribution of Bombyx mori silk fibroin; (a) Bombyx mori silk fibroin, (b) Molecular weight marker 200 kDa, (c) 66 kDa, and (d) 29 kDa
표 The molecular weight distribution of Antheraea pernyi silk fibroin; (a) Molecular weight marker 200 kDa, (b) 66 kDa, (c) 29 kDa, and (d) Antheraea pernyi silk fibroin
표 The effect of residual sericin content on the absorbance of the 5% regenerated silk formic acid solutions.
표 The effect of residual sericin content on the viscosity of 18% (w/w) regenerated silk formic acid solution.
표 The effect of residual sericin content on the FTIR spectra of the regenerated silk films.
표 Amino acid composition of the regenerated SF with various dissolution conditions
표 The effect of residual sericin content on water content of regenerated silk powder.
표 The relationship between shear viscosity and shear ratio of 18% regenerated silk formic acid solutions with different residual sericin content.
표 The effect of residual sericin content on the turbidity of the 5% regenerated silk formic acid solutions.
표 SEM photographs of electrospun silk fiber with various residual sericin contents.
표 The average diameter of regenerated silk by electrospun with different residual sericin content.
표 The effect of risidual sericin content on the breaking strength of electrospun silk fiber.
$X-ray diffractograms of electrospun regenerated silk with various risidual sericin content; (a) 0%, (b) 3.6%, (c) 11%, (d) 18.5%.$ 표 X-ray diffractograms of electrospun regenerated silk with various risidual sericin content; (a) 0%, (b) 3.6%, (c) 11%, (d) 18.5%.
표 TGA thermograms of electrospun regenerated silk with various residual sericin content; (a) 0%, (b) 3.6%, (c) 11%, (d) 18.5%.
표 Diet composition for experiment
표 Effect of diet supplementation of ssilk fibroin/sericin mixtures on body weight and Food intakes
표 Oragan and fat weights in high-fat diets supplemented with silk fibroin/sericin mixtures
표 Weekly change of diet supplementation of silk fibroin/sericin mixtures on the blood glucose concentration in high fat diet fed mice for 8 weeks
표 Weekly change of diet supplementation of silk fibroin/sericin mixtures on the plasma triglycerides concentration in high fat fed mice
표 Weekly change of diet supplementation of silk fibroin/sericin mixtures on the plasma total cholesterol concentration in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic GK activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic PEPCK activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic G6Pase activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic glucose regulating enzyme activities in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic FAS, ME, G6PD, CPT, β-oxidation activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic FAS activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic ME activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic G6PD activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic CPT activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic CPT activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic CAT, GSH-Px, GR, PON activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic CAT activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic GSH-Px activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic GR activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Hepatic PON activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Kidney PEPCK, G6Pase activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Kidney PEPCK activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Kidney G6Pase activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on erythrocyte CAT, GSH-Px, GR, activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Erythrocyte CAT activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Erythrocyte GSH-Px activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Erythrocyte GR activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on adipocyte FAS, ME, G6PD, CPT activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Adipocyte FAS activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Adipocyte ME activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Adipocyte G6PD activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Adipocyte CPT activity in high fat fed mice
표 Effect of diet supplementation of silk fibroin/sericin mixtures on Erythrocyte TBARS in high fat fed mice
표 Composition of the Experimental Diets
표 Body Weight Gain of Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder
표 Plasma, Liver and Fecal Lipid Profiles in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder
표 Plasma and Erythrocyte TBARS Concentration in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder
표 Hepatic Lipid-Regulating and Antioxidant Enzymes Activities in Mice Fed with High Fat Diet Supplemented with Antheraea pernyi Silk Fibroin Powder
표 Solubility test result of EMA 50 and NaCl in acetone/water mixture
표 The solubility test result of EMC products with different viscosity level in 85/15 acetone/water mixture.
표 The solubility test result of EMC 50 in methanol/ethanol mixtures
표 Summary of solubility test of EMC product in various organic solvents.
표 The solubility test result of HEMC products with various viscosity levels and substitution degrees in acetone/water mixtures.
표 Solubility of NaCl in acetone/water mixtures with different mixture ratio.
표 Ash content of HEMC and HPMC products after treatment in acetone/water mixture.
표 Yield of HEMC product with various viscosity levels.
표 Yield of HEMC and HPMC products
표 The FTIR spectra of HEMC films after refining process; (a) EMA 50U, (b) EMA 30U, and (c) EMA 80H.
표 The FTIR spectra of HEMC and HPMC films after refining process; (a) EMA 50U, (b) PMC 50U, and (c) EMA/EMC 50U
표 The relationship between shear viscosity and shear rate of 2% EMC aqueous solution with different molecular weights
표 The relationship between shear viscosity and shear rate of 2% EMA aqueous solution with different molecular weights
표 The relationship between shear viscosity and shear rate of 2% HEMC and HPMC aqueous solution with various substitution types and substitution degrees
표 Strain sweep test result of 2% EMC aqueous solution with various molecular weights
표 Strain sweep test result of 2% EMA aqueous solution with various molecular weights
표 Strain sweep test result of 2% HPMC and HEMC aqueous solution with different substitution degree
표 The effect of molecular weight on the frequency sweep test result of 2% HEMC aqueous solution
표 The effect of molecular weight on the frequency sweep test result of 2% HEMC aqueous solution
표 The effect of molecular weight on the frequency sweep test result of 2% HPMC and HEMA aqueous solution with different substitution degree
표 The effect of viscosity on the adhesion strength of 2% HEMC aqueous solution
표 The adhesion strength of 2% HPMC and HEMA aqueous solution with various substitution degrees
표 The relationship between shear viscosity and shear rate of diet paste with various molecular weight HEMC products at pH 7.4 solution.
표 The relationship between shear viscosity and shear rate of diet paste with various molecular weight HPMC and HEMC products at pH 7.4 solution.
표 Diet composition for experiment
표 Effect of diet supplementation of HEMC on body weight and Food intakes
표 Oragan and fat weights in high-fat diets supplemented with HEMC
표 Effect of diet supplementation of HEMC on the blood glucose change of mouse
표 Weekly change of diet supplementation of HEMC on the plasma triglycerides concentration in high fat fed mice
표 Weekly change of diet supplementation of HEMC on the plasma total cholesterol concentration in high fat fed mice
표 Effect of diet supplementation of HEMC on Plasma HDL-cholesterol, HTR and AI in high fat fed mice
표 Hepatic glucose regulating enzyme activities in mice supplemented with HEMC
표 Hepatic lipid regulating enzyme activities in mice supplemented with HEMC
표 Effect of diet supplementation of HEMC on Hepatic CAT, GSH-Px, GR, PON, SOD activity in high fat fed mice
표 Effect of diet supplementation of HEMC on Erythrocyte CAT, GSH-Px, GR, SOD activity in high fat fed mice
표 Effect of diet supplementation of HEMC on adipocyte FAS, ME, G6PD, CPT activity in high fat fed mice
표 Effect of diet supplementation of HEMC on Erythrocyte TBARS and Plasma TBARS in high fat fed mice
표 Plasma GOT and GPT levels in mice fed with high fat diet supplemented with HEMC
표 Hepatic glycogen content and plasma insulin concentration in mice fed with high fat diet supplemented with HEMC
표 Effect of diet supplementation of HEMC on plasma Leptin, Adiponectin, Resistin, TNF-α concentrations in high fat fed mice
표 Plasma Free fatty acid and Phospholipid concentrations in mice fed with high fat diet supplemented with HEMC

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