초록

Ⅲ. 연구개발의 내용 및 범위
땅콩으로부터 분리한 stilbene synthase (resveratrol synthase)를 벼에 형질전환하여 계통육성한 익산515와 526호의 안전성평가 재료로서의 적합성을 확인하기 위해 도입유전자의 완결성, 계통 고정도 및 농업적 특성, 레스베라트롤 생합성 쌀의 기능성 등을 확인하고 안전성평가에 적합한 이벤트를 선발하는 것이 본 연구의 1단계 연구 범위이다.

Abstract ▼

Two transgenic resveratrol rice lines of Iksan515 and Iksan526, which developed by NICS, RDA, were generated by expressing resveratrol synthase(RS) gene and selectable marker basta gene into commercial variety Dongjin under control of maize ubiquitin promoter. This study was conducted to evaluate th

Two transgenic resveratrol rice lines of Iksan515 and Iksan526, which developed by NICS, RDA, were generated by expressing resveratrol synthase(RS) gene and selectable marker basta gene into commercial variety Dongjin under control of maize ubiquitin promoter. This study was conducted to evaluate the events and select one of them for biosafety assessment. The molecular characterization of these two transgenic resveratrol rice lines were confirmed that two sets of T-DNAs were located on one locus in which they were connected inversely, in head-to-head orientation. The agronomic characteristics of resveratrol enriched ricies (Oryza sativa), Iksan515 and Iksan526, compared to a non-transgenic rice variety, Dongjinbyeo. The resveratrol enriched rices grown in three locations, Iksan, Milyang and suwon showed similar phenotypic traits. Although resveratrol has been known to have various health-beneficial effects, its effect on metabolic syndrome is most reliable (Liu et al, 2004 & Bordone et al, 2005). the purpose of our research was to evaluate and to confirm the efficacy of the resveratrol-enriched rice 526 (RS-526) and 515 (RS-515) on metabolic syndrome and its related diseases. Compared with the control, the RS-526 had a dramatic effect on metabolic syndrome and related diseases, reducing body weight, blood glucose, triglycerides, total cholesterol, and LDL-cholesterol by 24.7%, 22%, 37.4%, 27%, and 59.6%, respectively.
In addition , we have preliminarily assessed the potential risks of GM crops on human health and environment including the molecular characterization of Resveratrol synthase(RS) protein, the study to investigate the possible impact of resveratrol rice on the soil microbial community and the study of gene flow to determine the out-crossing rate of GM rice to cultivated rice for the 2nd phase assessment.
We should confirm and select event iksan526 available for the 2nd phase biosafety-assessment.

목차 Contents

• 표지 ... 1
• 제출문 ... 3
• 요약문 ... 4
• SUMMARY ... 6
• 목 차 ... 7
• 제1장 서 론 ... 8
• 제2장 국내외 기술개발 현황 ... 10
• 제3장 연구개발수행 내용 및 결과 ... 12
• 제4장 연구개발목표 달성도 및 대외기여도 ... 62
• 제1절 목표대비 달성도 ... 62
• 제2절 정량적 성과 ... 64
• 제5장 연구개발결과의 활용계획 ... 66
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 67
• 제7장 기타 중요 변동사항 ... 68
• 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 69
• 제9장 참고문헌 ... 70