초록 ▼

Ⅲ. 연구개발의 내용 및 범위
식량작물 유래 천연색소 물질의 물질정보수집, 데이타베이스를 구축하고 식량작물별 천연색소의 성분 추출, 정제 및 농축한 후 기능성 천연색소 물질의 분리/정제/화학구조분석 및 Chemical library를 구축한다. 그리고 생화학 및 분자생물학적 기법을 이용한 항당뇨, 항균 등의 기능성을 검정하고 생리활성검증을 통한 핵심화합물을 도출하고 이들 지표성분의 생리활성을 효소 및 세포수준에서 검증한다. 식량작물 유래기능성 천연색소 추출물은 동물실험을 통해서 항산화활성, 항염증활성 및 암예방효과를 측정하여

Ⅲ. 연구개발의 내용 및 범위
식량작물 유래 천연색소 물질의 물질정보수집, 데이타베이스를 구축하고 식량작물별 천연색소의 성분 추출, 정제 및 농축한 후 기능성 천연색소 물질의 분리/정제/화학구조분석 및 Chemical library를 구축한다. 그리고 생화학 및 분자생물학적 기법을 이용한 항당뇨, 항균 등의 기능성을 검정하고 생리활성검증을 통한 핵심화합물을 도출하고 이들 지표성분의 생리활성을 효소 및 세포수준에서 검증한다. 식량작물 유래기능성 천연색소 추출물은 동물실험을 통해서 항산화활성, 항염증활성 및 암예방효과를 측정하여 식품 및 화장품 소재로서 상품화 가능성을 검증한다.

Abstract ▼

The representation of color with natural dyes is closely related with the modern well-being life. With the aim to develop a natural colorant for use in cosmetics and food additives from a new source. Rice and sorghum are widely cultivated in the semi-arid torpics and subtropics and is a staple food

The representation of color with natural dyes is closely related with the modern well-being life. With the aim to develop a natural colorant for use in cosmetics and food additives from a new source. Rice and sorghum are widely cultivated in the semi-arid torpics and subtropics and is a staple food for the millions of people in Africa and Asia. The anthocyanin pigment of colored rice are mainly composed by cyanidin-3-O-glucoside. The other pigment fractions colored rice are identified to peonidin-3-O-glucoside and petunidin-3-O-glucoside. Four phenolic compounds, apigenin, apigeninidin, luteolinidin, and hesperidin were isolated from this methanolic extract of sorghum and sorghum by-product, and determined those structures by 1D and 2D nuclear magnetic resonance (NMR), including COSY and HMBC experiments. we have investigated the applicable extraction method to establish the optimum extraction process. The extraction solvents and extraction time were varied in order to explore the optimum condition. The optimum solvent concentration for the highest natural colorant extraction from sorghum were 100% methanol in 0.5 or 1.0% HCl. The optimum extraction time for the highest natural colorant extraction from sorghum was 24hours. The optimum extraction temperature for the highest natural colorant extraction from sorghum was 50℃. The natural color which it follows in storage temperature analyzed decomposition degree. The results show that the natural colorant degradation at room temperature and light condition were 8% and 16% after 20 and 30 days, respectively. However, the natural colorant was not broken down at cool temperature or dark condition. The extracts of sorghum(by-product), colored rice, and adzuki bean varieties showed inhibitory activity against mushroom tyrosinase and elastase. The tyrosinase inhibitory activities of the sorghum(by-product) varieties ranged from >500 to 27.5 ㎍/mL (IC₅₀). The higher whitening activities was 'hwanggeumchal sorghum' stem (IC₅₀, 37.0 ㎍/mL). The tyrosinase inhibitory activities of the colored rice varieties ranged from >500 to 39.07 ㎍/mL (IC₅₀). The highest whitening activities were observed in ‘heugseol’ (IC₅₀, 39.07 mg/mL). The elastase inhibitory activities of the sorghum varieties ranged from >400 to 56.03 ㎍/mL (IC₅₀). However, the extracts of colored rice and adzuki bean not showed at elastase inhibitory activity. A methanol extract of sorghum was evaluated for its inhibitory effect on recombinant virus H1N1 neuraminidase. The influenza neuraminidase (E.C. 3.2.1.18) is an antiviral target of high pharmaceutical interest because of its essential role in cleaving sialic acid residues from the surface of glycoproteins and facilitating release of virions from infected cell. The extracts of sorghum inhibited H1N1 neuraminidase with an IC₅₀ value of 1.75 μg/mL. The methanol extract of sorghum showed very strong inhibitory effect and four phenolic compounds were confirmed by spectroscopic data. Among them, in addition to isolation of luteolin and apigenin which is known as neuraminidase inhibitor of natural compound, we ascertained that apigenindin and luteolinidin have good inhibitory effect on enzyme activity. In kinetic analysis, four compounds were showed non-competitive inhibition mechanism. This study suggested that sorghum extract and isolated compounds can be neuraminidase inhibitors in case of the infection and spread of influenza virus and kinds of bacterial diseases causing by neuraminidase. So, sorghum grains can be used as a food source for promotion of health and a feed for livestock. The result indicates that colored rice, sorghum and sorghum by-product can be used as a good natural colorant resources.

목차 Contents

• 표 지 ... 1
• 제 출 문 ... 2
• 요 약 문 ... 3
• S U M M A R Y ... 4
• 목 차 ... 6
• 제1장 서 론 ... 7
• 제2장 국내외 기술개발 현황 ... 9
• 제3장 연구개발수행 내용 및 결과 ... 10
• 제1절 식량작물 • 부산물 유래 기능성 천연색소 Chemical library 구축 및 대량추출법 개발 ... 10
• 제2절 기능성 천연색소의 생리활성 검정을 통한 후보물질 탐색 및 선발 ... 19
• 제3절 식량작물 및 부산물 유래 기능성 천연색소 성분의 안정성 및 동물실험을 통한 효능평가 ... 26
• 제4장 연구개발목표 달성도 및 대외기여도 ... 34
• 제1절 목표대비 달성도 ... 34
• 제2절 정량적 성과 ... 35
• 제5장 연구개발결과의 활용계획 ... 39
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 39
• 제7장 기타 중요 변동사항 ... 40
• 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 40
• 제9장 참고문헌 ... 41