보고서 정보
주관연구기관 |
세종대학교 산학협력단 Sejone university |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2013-01 |
과제시작연도 |
2012 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201300014381 |
과제고유번호 |
1395028529 |
사업명 |
농식품위해요소안전관리기술개발및평가체계구축 |
DB 구축일자 |
2013-08-26
|
DOI |
https://doi.org/10.23000/TRKO201300014381 |
초록
Ⅲ. 연구개발의 내용 및 범위
1. 국내산 농산물 및 자생식물의 유효성분 분석
2. 소화과정 중 중금속 흡착 및 흡수 억제 효과를 보이는 국내산 농산물 및 자생식물의 선정 및 효능 규명
3. 중금속으로 기인한 간 세포 독성 및 산화적 손상 억제 효과를 보이는 국내산 농산물 및 자생식물의 선정 및 효능 규명
4. 중금속 흡착, 중금속에 의한 세포독성 및 산화적 손상 억제에 효과적인 식물성 천연추출물의 유효물질 규명
5. 제품개발
Abstract
▼
Effect of leek (Chinese chive) on bioaccessibility of arsenic (As), cadmium (Cd), and lead (Pb) was determined in comparison with sodium copper chlorophyllin (SCC) using in vitro digestion model. Leek (0, 6, 12, 60, and 120 mg) and SCC (0, 1, 5, 10, and 50mg) were digested with equal concentration (
Effect of leek (Chinese chive) on bioaccessibility of arsenic (As), cadmium (Cd), and lead (Pb) was determined in comparison with sodium copper chlorophyllin (SCC) using in vitro digestion model. Leek (0, 6, 12, 60, and 120 mg) and SCC (0, 1, 5, 10, and 50mg) were digested with equal concentration (10 ppm) of As, Cd, and Pb.
Concentration of each heavy metal in aqueous phase following in vitro digestion was measured using an inductively coupled plasma optical emission spectrometer. Changes in absorbance spectra of chlorophyll extracted from leek and SCC by heavy metals were measured at 0, 1, 2, and 3 h after mixing. Results showed that the concentration of each heavy metal in aqueous phase decreased with increasing amounts of leek and SCC. At the highest level of leek (120 mg), the concentrations
of As, Cd, and Pb decreased to 93.9, 87.1, and 58.2%, respectively. Absorbance of chlorophyll drastically decreased after 1 h of mixing with each As, Cd, and Pb, and no difference in the absorbance was observed after 2 and 3 h. This result indicates
that mixing chlorophyll extracted from leek with each heavy metal for 1 h was sufficient for chelating As, Cd, and Pb ions. On the other hand, SCC and each heavy metal showed a decreasing pattern of absorbance without any significant difference
for 3 h, indicating that chlorophyll from leek was more effective than SCC, a commercial grade chlorophyll derivative, in chelating As, Cd, and Pb. Results showed leek reduces heavy metals in humans.
The three-stage of in vitro digestion model system simulating human digestive fluids was employed to determine changes in the profile of phenolic compounds, antioxidant activity and total phenolic content by digestion. The composition of total
phenolic compounds in leaf, root and stem detected by gas chromatography-mass Spectroscopy method was 44.27, 19.31 and 8.59%, respectively, and 3-hydroxybenzoic acid was found as a predominant phenolic compound identified in all parts of Smilax china. Both 2, 2-diphenylpicrylhydrazyl free radical scavenging effect (8.66 mg/mL for IC50) and total phenolic content (2,383.55 mg gallic acid equivalent/ mL) using Folin–iocalteu method were remarkably high in leaf of Smilax china. Bioaccessible
antioxidative effect and digestive stability of total phenolic content in leaf were also significantly higher than those of other parts.
Last, This study was conducted to investigate the effect of water spinach on bioaccessibility and intestinal uptake of heavy metals (Arsenic (As), Cadmium (Cd), and Lead (Pb)) using an in vitro digestion model with Caco-2 cells. Aliquots of each heavy
metal were co-digested with each part of water spinach (stem and leaf) and then cultured with Caco-2 intestinal cells for 1 h at 37 C. Each heavy metal of As, Cd, and Pb was quantified using an ICP-OES. As the amount of stem and leaf (10, 50,
100, and 500 mg) of water spinach increased, bioaccessibility of As, Cd, and Pb decreased to 42.63%, 12.04%, and 26.17% by leaf and 30.37%, 43.27%, 40.07% by stem, respectively. Intestinal uptake of As, Cd, and Pb reduced to 65.8%, 25.7%, and 44.8% for leaf (500 mg) and 48.4%, 51.3%, and 64.3% for stem (500 mg), respectively, compared with the control without leaf and stem. The leaf from water spinach was the most effective for decreasing both bioaccessibility and cellular uptake of Cd.
목차 Contents
- 표지 ... 1
- 제출문 ... 2
- 요약문 ... 3
- SUMMARY ... 5
- 목차 ... 7
- 제1장 서론 ... 8
- 제1절 연구개발 목적 ... 8
- 제2절 연구 필요성 ... 8
- 제2장 국내외 기술개발 현황 ... 9
- 제3장 연구개발수행 내용 및 결과 ... 10
- 제1절 국내산 농산물 및 자생식물의 소화과정 중 중금속 흡착 효과 ... 10
- 제2절 국내산 농산물 및 자생식물의 중금속으로 기인한 간 세포 독성 저해 효과 ... 21
- 제3절 국내산 농산물 및 자생식물의 중금속으로 기인한 간 산화적 손상 억제 효과 ... 30
- 제4장 연구개발목표 달성도 및 대외기여도 ... 61
- 제1절 목표대비 달성도 ... 61
- 제2절 정량적 성과 ... 62
- 제5장 연구개발결과의 활용계획 ... 65
- 1. 활용계획 ... 65
- 2. 산업재산권 출원 사항 ... 65
- 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 66
- 제7장 기타 중요 변동사항 ... 67
- 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 68
- 제9장 참고문헌 ... 69
- <참고> 주요 연구성과 요약 ... 72
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