초록

Ⅲ. 연구개발의 내용 및 범위
○ 국내 블루베리 주요 병해충의 발생생태 구명
○ 블루베리 주요 병해충의 발생생태에 기초한 친환경 방제기술 개발
○ 블루베리 해충 방제약제 선정시 천적에 대한 독성이 적은 약제 선발
○ 블루베리 병해충 검색을 위한 expert system 개발
○ 블루베리 주요 바이러스 병의 효율적 진단법 개발

Abstract ▼

In Korea, blueberry cultivation duration had been only a few years. But currently, the cultivation area has suddenly increased and reached to 1,082 ha. A few Diseases occurrence has appeared, because of short blueberry cultivation duration. But, the diseases occurrence ratio and disease type have be

In Korea, blueberry cultivation duration had been only a few years. But currently, the cultivation area has suddenly increased and reached to 1,082 ha. A few Diseases occurrence has appeared, because of short blueberry cultivation duration. But, the diseases occurrence ratio and disease type have been increasing because the cultivation duration and the areas have been increasing. So the diseases that are difficult to control might appear. Recently, most of the blueberry cultivation farmers are cultivating with none-agrichemical environment-friendly. Therefore, if the diseases occur, it might be difficult to control the diseases.
We conducted nationwide survey on the occurrence of major insect pests, its damage status and management practice of environment-friendly managed blueberry farm. Survey was conducted from March to October across two year span from 2010 to 2012 in Hoengseong, Goyang, Pyeongtaek, Cheonan, Cheongwon, Gochang, Sunchang, Jeongeup which are major blueberry cultivation areas in Korea. Dominant insect pests were Dasineura oxycoccana,Scirotothrips dorsalis, Ricania sp., Blitopertha orietalis, Monema flavescens, Aphis spiraecola and etc. Their occurrence and damage were very different depending on cultivation method and surrounding environment. Also we conducted several basic experiments to establish environment-friendly management practice on the blueberry gall midge and other major insect pests.
All collected leaf samples from commercial orchard of blueberry were tested by ELISA or PCR for the presence of viruses. Results showed that the virus infection rate was 9.6%. The highest infection rate of blueberry samples was by far BlShV 7.1% followed by BlScV 5.0%, BSSV 1.9% and BRRV 0.3%. However, RT-PCR or PCR testing carried out for the virus positive samples confirmed the presence of BRRV. The BRRV of the Soymovirus genus in the Caulimovididae family causes red ringspot diseases in Vaccinium corymbosum, V. formosum, V. macrocarpon on leaves, stems and fruits. The virus has been identified in United States, Japan, Czech Republic, Slovenia and Poland. In July of 2010, highbush blueberry with red ringspots on leaves and circular blotches on ripening fruits was found in one plant of cv. Duke in Pyeongtaek, Korea. The symptoms were similar to red ringspot disease caused by BRRV. Red ringspots of leaves on the upper surface were the most visible symptom and were increased when the leaves matured in late summer through fall. To confirm the presence of BRRV, the leaves of symptomatic plant were collected and tested by developed PCR technique. BLAST analyses of obtained fragments revealed 97% nucleotide sequence identity and 98% amino acid identity with the reverse transcriptase
gene of known BRRV isolates in the GenBank. The virus transmitted by grafting and cutting from infected mother plant. Blueberries are usually propagated by cutting, so BRRV infected plants should be remove and destroy. Also, we developed RT-PCR technique for detection of economically important viruses, BlScV and BlShV in bluberries.
Also we conducted several basic experiments to establish environment-friendly management practice on the blueberry major disease.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 5
• 목차 ... 7
• 제1장 서론 ... 8
• 제2장 국내외 기술개발 현황 ... 9
• 제1절 국내 연구 현황 ... 9
• 제2절 국외 연구 현황 ... 9
• 제3장 연구개발수행 내용 및 결과 ... 11
• 제1절 블루베리 주요 병해 발생실태 조사 및 친환경 방제법 연구 ... 11
• 제2절 블루베리 주요 해충 발생실태 조사 및 친환경 방제법 연구 ... 18
• 제3절 블루베리 주요 바이러스병 발생실태조사 및 관리모델 개발 ... 44
• 제4장 연구개발목표 달성도 및 대외기여도 ... 51
• 제1절 목표대비 달성도 ... 51
• 제2절 정량적 성과 ... 52
• 제5장 연구개발결과의 활용계획 ... 53
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 54
• 제7장 기타 중요 변동사항 ... 55
• 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 56
• 제9장 참고문헌 ... 57
• 참고 주요 연구성과 요약 ... 61