초록 ▼

○ 연구결과
- 인디고 천연소재 제품개발 및 천연염색 산업화
․ 염료 생산/염색공정 생산 자동화
․ 인디고 추출 및 최적화 조건 개발
․ 인디고 유래별 최적 염색 공정 확립
․ 천연염색 디자인상품 개발
․ 인디고 바이오 하우징 천연소재 개발
․ 인디고 천연염색 장치 개발 및 제품 산업화
․ 천연원료를 이용한 기능성 천기저귀 제품개발
․ 천연염색 한지섬유 등 천연소재 국내외시장 진입과 홍보 및 마케팅
․ 국내외 전시회 및 박람회를 통한 제품의 홍보와 판매시장 확대
- 농학 및

○ 연구결과
- 인디고 천연소재 제품개발 및 천연염색 산업화
․ 염료 생산/염색공정 생산 자동화
․ 인디고 추출 및 최적화 조건 개발
․ 인디고 유래별 최적 염색 공정 확립
․ 천연염색 디자인상품 개발
․ 인디고 바이오 하우징 천연소재 개발
․ 인디고 천연염색 장치 개발 및 제품 산업화
․ 천연원료를 이용한 기능성 천기저귀 제품개발
․ 천연염색 한지섬유 등 천연소재 국내외시장 진입과 홍보 및 마케팅
․ 국내외 전시회 및 박람회를 통한 제품의 홍보와 판매시장 확대
- 농학 및 생물 공학적 기법을 이용한 쪽의 인디고 생산 최적화
․ 인디고 고함유 쪽 재배 및 가공기술 개발
․ 인디고 생산 작물 분자 육종
․ 쪽 식물의 elicitation을 통한 인디고 생산성 극대화 및 인독실 합성효소 규명
․ Indigo환원 미생물 선발 및 대사공정 개발

Abstract ▼

• Line facilities for the Naju eco-friendly dyeing factory in the cultivated area for the efficient extraction of the indigo plant is expected to be introduced.
• Establish the pattern indigo dyeing technology for development and diversification of natural dyeing, the dyeing process. The natural

• Line facilities for the Naju eco-friendly dyeing factory in the cultivated area for the efficient extraction of the indigo plant is expected to be introduced.
• Establish the pattern indigo dyeing technology for development and diversification of natural dyeing, the dyeing process. The natural dyeing education, experience and natural dyeing treatment and welfare can be used. Natural dyed products to take advantage of this technology to improve the marketability and sales by diversifying plan activation.
• Natural Dyeing Cultural Product Development : My hand to fashion garment indigo dyeing indigo dyeing package development and product development. The indigo dyeing Package students, without an adult will be easily used in the dyeing experience and can contribute to the local economy, promote diversity and sale of natural dye products enable the development of highly functional, high-value-added indigo dye shirt.
• Overall, the results in the Polygonum tinctorium extract of human cancer cells by MTT assay for cytotoxicity of the Polygonum tinctorium extract of the larynx cells, in cancer cells, breast cancer cells than in cervical cancer cells, liver cancer cells, colon cancer cells in a relatively higher toxicity was.
• Results of the analysis of the antimicrobial activity of indigo-dyed fabric dyeing conditions, using glucose as a reducing agent to the alkaline conditions niram and indigofera the antimicrobial activity of the dyed fabric was from 97.0 to 98.3%.
• The experimental results for the dyeability of the fabric according to the dyeing temperature and ethanol content of indigofera and Niram, generally containing 20% ethanol at 50℃ with the dye to dye dyeability of the fabric was.
• Investigated the antibacterial activity of the fabric of indigo dye from 99.0 to 99.9%, regardless of the type of indigo dye cotton fabrics showed antimicrobial activity. Silk Isatis tinctoria showed 67.5% to 87.8% Indigofera showed 10.5% to 81.1%,, Niram showed 67.0% to 99.5%, synthetic indigo, 0.0 and 85.4%, respectively. Of silk fabrics dyed with Niram highest antimicrobial.
• As the well-being-oriented trend that regards highly to health and environment and to improving the quality of life has been spreading worldwide, the products that embody eco-friendly environment, health, and personal customization are leading the consumption in all fields of the necessities of life. Natural dyeing materials, especially, are highly preferred by the consumers since they are eco-friendly, have features of antibacterial, deodorizing, and skin protective functions, and carry the esthetic characteristic caused by its distinct color formation. One of the various materials for natural dying, natural indigo, is beneficial for humans not only because it is environmentally sustainable but also has functionalities such as expressing the colors of the nature. In addition, natural indigo has high developmental values in all aspects such as stain ability, color, and fastness property, but commercialization of the product is insufficient despite the fact that it is the most highly consumed dyeing material of all. Therefore, package design strategy that holds the images of the natural indigo and its emotional image of the brand should be developed to merchandise the high value of the indigo product.
• Hence, this study contributes to increase the revenue of farmhouse cultivation and regional economic vitalization through B.I. applied package design development that makes the most of natural dyeing products’ characteristics. The first step of the package design development took place by understanding the main content about the developed item and its design direction through survey of demand on the brand’s package design. Then the brand and the target, and the natural dyeing market were analyzed. After that, the developmental direction on the package design keyword and concept was set up and, finally, the package design was established.
• The package design development designed three kinds of 9 sets of packages for scarves, ties, and shopping bags for each three design concepts, and developed four kinds of accessories such as wrapping papers, stickers, labels, and explanatory notes as experimental products. Furthermore, the six kinds of package designs that were produced as experimental products are applied for a patent and the two kinds of the accessories from the package design are patent pending. The research paper, “The development of sleeping gears using natural indigo dyeing” was submitted to the Korean Cultural Design Society and published on the journal Vol.18 No.3.
• This research study will be able to improve the image of the dyeing brands and products in the Naju region by attempting the high sensitive package design development. It will also provide the direction to the local government and natural dyeing companies that are promoting the natural dyeing business, and give assistance in establishing a distinguished marketing strategy.
• In recent years, textile dyeing using natural pigment has gained lots of attention in the global market. The reason is natural pigment like indigo has been traditionally and world widely used in the human history. There are many affords and researches in order to develop modern ways to use natural pigments. Therefore, the demand for natural dyeing business is growing steadily, but the mass production of natural dyeing has not occurred yet. Thus, the supply is not smooth.
• This study is targeting revenue growth of natural dyes, plant growers and the local economy. The process is as follows. First, analyze design and consumer status. Second, development of modern living accessories and interior finishes. Final is the development of B.I and package design.
• In the first year of research, the development of dyeing fabrics available for interior finishes based on consumer preference analysis, and natural dye products will be preceded. In addition, the indigo dyeing pattern design development for the production of differentiated products will be followed. Indigo color dyeing development which has been a difficult reason for mass production will be included.
• In the second year of research, the goal of development is designing patterns to be applied to the fabric dyeing, based on the results of the earlier analysis. From the basic fabric patterns promote the development of products that can be applied to the interior environment.
• In the third year, B.I and the development of the package design can be imprinted with a unique image of the indigo dye products. Thus, the indigo dyed fabrics to inform a lot of people.
• Title : Developing functional cloth diapers made by natural ingredients ŸPurpose for research and develop and needs
- Written from technical aspect and environmental aspect
- Developing cloth diapers with natural ingredient from indigo, at the same time it is necessaryto develop a diversity of products which are highly accessible.
• The contents for R&D and ranges
- Developing panty type cloth diapers
- Developing a diversity of products with fabric colored with indigo (the bib/spit pot)
• The result of R&D
- Promoting with many kinds of media with developed products (Exhibition, online etc.)
- On the process with two design application products.
• The plan for outcome of R&D and using output
- Planning to have a possibility to enter the market with developed products.
- Planning to expand a range of consumer with overseas/domestic exhibition.
• The purpose of this study is to increase productivity of indigoid pigments in indigo-accumulating plants in the field as well as in vitro by biotechnological approaches. In particular, the study aims (1) to find condition maximizing the indigoid-producing metabolism in indigo plant in the field and in vitro conditions and
(2) to breed the indigoid-producing plant by introducing useful foreign gene.
• The scope of the present study is (1) to develop practical method to dramatically enhance accumulation of indigoid pigments in the farm condition, (2) to clone indole-producing enzyme, indole-3-glycerol phosphate lyase (IGL), from Polygonum tinctorium, (3) to establish in vitro culture of P. tinctorium for indirubin production and molecular breeding, and (4) to elucidate molecular origin of oxygen in indole oxidase reaction.
• We successfully cloned two putative IGL clones from P. tinctorium and developed E. coli mutant that can verify function of IGL by complementation method. Currently, introduction of IGLs into the E. coli mutant is on-going. To characterize the indole-oxidizing enzyme in P. tinctorium, origin of oxygen atom in indigo was established as molecular oxygen, thus suggesting involvement of P450 in the process.
Most important finding in this research was to establish practical method to increase indigoid production upto 100% by application of so-called plant defense activator. This should contribute to indigo-plant growers’ income in Korea. Also we established hairy root culture that accumulated indirubin, and demonstrated introduction of foreign gene to hairy root. Nevertheless, introduction of bacterial indole oxidase did not affect pigment production by hairy root.
• This study was conducted to suggest the standard cultivation practices and develop new varieties for enhancing the fresh leaf yield and colorant level of an indigo crop, Persicaria tinctoria H. Gross, containing the blue dye indigo.
• Seed germination rate was high at 15 or 20℃ constant, and 25/15℃ (day/night) alternative temperatures, with irrespective of seed with pericarp. Proper solvent for indigo standard was dimethyl sulfoxide, and appropriate concentration was 1 mg of indigo in over 10 mL of DMSO. In results of seasonal changes of growth characteristics and colorant level, fresh leaf yield was increased to day 85 after transplanting, and then remained almost constant. As being later stages of plant growth, branch number increased, and leaf area and the ratio of leaf to total shoot of dry weight decreased.
Contents of indican and indigo increased till Aug. 10, and then decreased during further plant growth. Indican content with different leaf position was higher in top leaves than low leaves. As transplanting time was delayed, fresh leaf yield were maximum at earliest transplanting (May 30) and then decreased, while Niram (blue dye extract) and indigo content of fresh leaf remained almost constant though showing a slight fluctuation. As harvest time was delayed, fresh leaf yield changed increasingly, while Indigo level increased largely to Aug. 5, and then continuously decreased with more delayed harvest. The results demonstrated that earlier transplanting before May 30 and harvest in early August were appropriate for producing higher fresh leaf yield and colorant level. Leaf yield of twice-harvest was higher than one of once-harvest. Level of colorant was higher on Aug. 10 or Aug. 20, showing no difference between twice-harvest and once-harvest. It could be better for production of larger colorant with twice-arvest to have longer growth period before flowering by earlier transplanting.
Two row planting and one planting hills in Naju Local, and two row planting and two planting hills in NaramBlue were optimum planting densities to enhance leaf yield and indigo content.
• In progeny test of selfing individual, there was no great variation of growth characteristics in next generation and was no large difference between self-pollinated and open-pollinated population from single plant, so this plant can be supposed to be an autogamous crop. A mass-selected line, Naju No. 2, had good traits such as relatively erect type, large leaf area with round leaf type, having higher ratio of leaf to total shoot of dry weight and larger amount of colorant level. Naju No. 2 was recognized as superior variety through regional yield trial and yield performance in farm field, and was applied for new variety named 'NaramBlue'. PTLW-2 line with white flower and late maturity was bred by pure line separation, and this line was applied for new cultivar named 'Baekwharam'. Major selection characters in breeding program was late maturity, plant vigor such as branching and plant height, leaf characteristics such as size and thickness, and flower color. A total of 88 lines including PT-1 were selected, and 32 lines including B1 were tested in performance trial, and then 25 lines would be tested in yield trials. Selection in further breeding program will be specially focused on maturity, leaf characteristics, and flower color, and then uniformity and genetic stability of selected population would be evaluated.
• Cultivation practices such as transplanting time, harvest time, planting density, harvesting system could be used in farm households, and new varieties would be also supplied to farmer for producing the quality blue dye materials with high biomass production.
• The draft genome sequence of Pseudomonas sp. G5 was produced by using Illumina MiSeq sequencing system (Illumina,, USA). The sequencing library was constructed by Illumina TruSeq DNA sample preparation kit v2 following the manufacturer's instructions. 16S rRNA gene-based similarity search revealed that G5 showed 100% pairwise similarity to Pseudomonas umsongensis Ps 3-10T. The second hit was Pseudomonas mohnii Ips-2Twith 98.8% similarity. However, we could not identify our isolate to the species level, because the genome sequence information of species showing the highest similarity to our strain is not shown. Based on ANI analysis, the closest genome in public database was Pseudomonas sp. GM78 isolated from rhizosphere environment.
• FAD-dependent enzymes involved in carbohydrate transport and catabolism are found in genome of strain G5. It is reported that indigo reduction takes place as a result of electron transfers following the transformation of glucose to pentose 5-phospate by a mediator enzyme containing FAD+ as the cofactor(Takara et al., 1962).
• The genome sequence of Pseudomonas sp. strain G5 will contribute to the understanding of the adaptation of this species to indigo reduction process and may be considered to provide a biological tool to enhance efficiency in the traditional indigo dye industry by an environmental friendly manner.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 14
• CONTENTS ... 19
• 목차 ... 22
• 제1장 연구개발과제의 개요 ... 25
• 제1절 연구개발의 목적 및 필요성 ... 25
• 1. 연구개발의 목적 ... 25
• 2. 연구개발의 필요성 ... 25
• 제2절 연차별 연구개발의 범위 ... 28
• 제2장 국내외 기술개발 현황 ... 30
• 제1절 국내 기술개발 현황 ... 30
• 제2절 국외 기술개발 현황 ... 36
• 제3장 연구개발수행 내용 및 결과 ... 38
• 제1절 인디고 함유 (쪽) 재배 생산 기술 ... 38
• 1. 쪽(Persicaria tinctoria) 집단내의 유전자 다양성분석 ... 38
• 2. 쪽 종자발아 특성 ... 47
• 3. 쪽 재배시기별 생육특성 변화 ... 48
• 4. 쪽 유망계통 선발시험 ... 52
• 5. 쪽의 지역별 생육 및 수량을 비교 ... 53
• 6. 품종보호 출원한 신품종 ‘나람블루’의 품종적 특성 ... 60
• 7. 쪽 재배방법 개발시험 ... 61
• 8. 쪽 우량계통 육성연구 ... 77
• 제2절 생물공학적인 인디고 생산 최적화 기술 ... 82
• 1. 인돌 산화 효소의 특성 규명 ... 82
• 2. 인돌 합성효소의 cloning ... 85
• 3. 화학적 식물활성화제 처리에 의한 인디고 축적 증대 효과 ... 93
• 4. 모상근 배양에 의한 대사공학적 indirubin 생산 ... 98
• 5. Indole oxygenase의 과발현을 통한 Indirubin과 Indigo 생산 ... 106
• 제3절 인디고 환원 미생물 선발 및 대사 공정 개발 ... 110
• 1. 인디고환원 미생물선발 ... 110
• 2. 인디고 환원 미생물의 분리 및 특성 ... 113
• 3. 분리된 인디고 환원 미생물의 인디고 환원 능력 ... 114
• 4. 인디고 환원에 영향을 미치는 pH와 온도 ... 115
• 5. 선발된 미생물을 이용한 환원력 향상과 분리된 미생물의 유전체 정보 획득 ... 116
• 제4절 인디고 식물의 생리활성 및 항 아토피염 효과 ... 122
• 1. 인디고 염료 항아토피 및 피부 항염 효능 평가 ... 122
• 2. 대청 추출물의 항산화 효소 활성 및 항균 효과 ... 130
• 3. 쪽 부위별 추출물의 항암효과 ... 138
• 제5절 인디고염료 공정생산 기술 산업화 ... 143
• 1. 쪽과 인디고페라의 잎 추출물의 염색성 ... 143
• 2. 요소의 첨가가 인디고의 환원에 미치는 영향 ... 150
• 3. 인디고 염색공정 축소 및 소요시간 단축에 관한 기술개발 ... 162
• 4. 인디고 염료와 식물성 색소(양파껍질, 소목)를 이용한 다컬러 대량 염색 공정 확립 ... 189
• 5. 쪽을 이용한 블루색상을 가지는 가죽 염색공정 ... 207
• 제6절 인디고염색 최적화 및 염색공정 장치 개발 ... 239
• 1. 생산 기반 확대와 기술개발 ... 239
• 2. 천연염색 기술개발 및 염색재현성 ... 257
• 3. 염료 제어기술 및 시제품의 시운전(data base) ... 290
• 4. 실크 인디고염색 공정 data base ... 294
• 5. 섬유에 따른 인디고염료 흡수 및 고착 반응염색 ... 297
• 6. 쇼핑-수세 공정 기술 개발 ... 301
• 7. 자동화시설 및 연속공정 ... 309
• 8. 대량 3,000yd 연속공정 및 시제품 개발 ... 320
• 제7절 천연염색 디자인상품 개발 ... 321
• 1. 텍스타일 디자인 개발 과정 ... 321
• 2. 텍스타일 디자인 개발 ... 321
• 3. 고감성.고기능성 천연염색제품 디자인 개발 ... 336
• 4. 천연염색 패키지 디자인 및 시제품 개발 ... 357
• 제8절 바이오하우징 천연소재 개발 및 제품 마케팅 ... 375
• 1. 인테리어제품 분석하여 연구하고자하는 제품 선정 ... 375
• 2. 패턴 디자인 분석 ... 375
• 3. 패턴 디자인 개발 ... 376
• 4. 디자인한 패턴을 적용하여 시제품 제작 ... 379
• 5. 바이오하우징 천연소재 개발 및 제품 마케팅 개발 세부 내용 ... 383
• 6. 천연벽지 시제품 개발 ... 391
• 제9절 천연원료를 이용한 기능성 천 기저귀 제품 개발 ... 392
• 1. 팬티형 천기저귀 디자인 개선 및 시제품 개발 ... 392
• 2. 연구 내용 ... 404
• 3. 연구개발과제의 개요 ... 408
• 4. 연구개발의 목적 ... 409
• 5. 국내외 기술개발 현황 ... 410
• 6. 연구개발수행 내용 및 결과 ... 413
• 제10절 인디고 염색물의 상품화 및 판매 활성화 ... 418
• 1. 내손으로 하는 쪽 염색 패키지 개발 ... 418
• 2. 인디고 염색 패션의류상품 개발 ... 420
• 제4장 목표달성도 및 관련분야에의 기여도 ... 426
• 제1절 평가의 착안점 및 기준 ... 426
• 제2절 목표달성도 및 관련분야에의 기여도 ... 428
• 1. 제1-1과제 인디고 염료 공정 생산 기술 개발 및 산업화 ... 428
• 2. 1-2과제 천연염색 디자인 상품 개발 ... 435
• 3. 1-3과제 인디고 염색 최적화 및 염색공정 장치 개발 ... 443
• 4. 1-4과제 바이오하우징 천연소재 개발 및 제품 마케팅 ... 448
• 5. 1-5과제 천연 원료를 이용한 기능성 천기저귀 제품 개발 ... 455
• 6. 2-1과제 생물공학적인 인디고 생산 최적화 ... 459
• 7. 2-2과제 인디고 함유식물(쪽) 재배생산기술 ... 464
• 8. 2-3과제 인디고 환원 미생물 선발 및 대사공정 개발 ... 470
• 제5장 연구개발 성과 및 성과활용 계획 ... 473
• 제1절 연구개발 성과 ... 473
• 1. 인디고 염료공정 생산 기술개발 및 산업화 ... 473
• 2. 천연염색 디자인상품 개발 ... 475
• 3. 인디고 염색 최적화 및 염색공정 장치개발 ... 475
• 4. 바이오하우징 천연소재 개발 및 제품 마케팅 ... 476
• 5. 천연원료를 이용한 기능성 천기저귀 제품개발 ... 476
• 6. 생물공학적인 인디고 생산 최적화 ... 476
• 7. 인디고 함유 식물(쪽) 재배 생산 기술 ... 477
• 8. 인디고 환원 미생물 선발 및 대사공정 개발 ... 479
• 제2절 성과활용 계획 ... 480
• 1. 인디고 염료공정 생산 기술개발 및 산업화 ... 480
• 2. 천연염색 디자인상품 개발 ... 481
• 3. 인디고 염색 최적화 및 염색공정 장치개발 ... 481
• 4. 바이오하우징 천연소재 개발 및 제품 마케팅 ... 482
• 5. 천연원료를 이용한 기능성 천기저귀 제품개발 ... 482
• 6. 생물공학적인 인디고 생산 최적화 ... 482
• 7. 인디고 함유 식물(쪽) 재배 생산 기술 ... 483
• 8. 인디고 환원 미생물 선발 및 대사공정 개발 ... 484
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 485
• 1. 일본 도쿠시마의 쪽문화와 산업 현황 ... 485
• 2. 인도의 쪽염색 문화와 산업 현황 ... 486
• 3. 대만.타이완의 쪽염색 문화와 산업 현황 ... 487
• 4. 인디고 생합성에 대한 연구 현황 ... 489
• 5. 천기저귀 산업 현황 ... 489
• 제7장 참고문현 ... 491
• 연구개발보고서 초록 ... 497
• 끝페이지 ... 499