초록 ▼

▶ 연구목적 :
○ 차나무 친환경 재배면적의 증가에 따라 병해 피해가 해마다 증가하고 있어 이에 대한 친환경 병 방제 기술로 안정적인 녹차를 생산하고자 함
○ 차나무 잎마름병 및 떡병의 발생생태 조사 및 병 방제를 위한 친환경농자재 발굴 및 개발을 통해 환경친화적 방제체계 확립
▶ 주요연구내용 :
○ 1세부과제명 : 차나무잎마름병 및 떡병 발생생태 조사
- 차나무 잎마름병 및 떡병 발생소장 및 피해율조사
- 차나무 잎마름병 및 떡병 발병조건 구명
- 차나무 잎마름병 및 떡병 발생생태 조사

▶ 연구목적 :
○ 차나무 친환경 재배면적의 증가에 따라 병해 피해가 해마다 증가하고 있어 이에 대한 친환경 병 방제 기술로 안정적인 녹차를 생산하고자 함
○ 차나무 잎마름병 및 떡병의 발생생태 조사 및 병 방제를 위한 친환경농자재 발굴 및 개발을 통해 환경친화적 방제체계 확립
▶ 주요연구내용 :
○ 1세부과제명 : 차나무잎마름병 및 떡병 발생생태 조사
- 차나무 잎마름병 및 떡병 발생소장 및 피해율조사
- 차나무 잎마름병 및 떡병 발병조건 구명
- 차나무 잎마름병 및 떡병 발생생태 조사
○ 2세부과제명 : 차나무 잎마름병 및 떡병에 대한 환경친화적 방제기술 개발
- 차나무 잎마름병 및 떡병 방제용 친환경자재 선발을 위해 시판 중인 미생물제제 및 천연물농약 중 18종을 선발하여 항균활성효과 조사 및 제품 품질평가(Q/C)
- 차나무 잎마름병 및 떡병 방제용 친환경농자재 제품 개발을 위해 미연구 지의류내생균으로부터 길항균주 선발
- 지의류내생균의 항균활성물질 대량생산 최적 배양 시스템 및 항균 물질 규명
○ 3세부과제명 : 차나무잎마름병 및 떡병에 대한 환경친화적 방제체계 확립 및 농가실증
- 시판중인 친환경제제의 항균활성도의 조사 결과를 바탕으로 친환경 농자재 6종을 선발, 온실 및 포장에서 약제 효과 검정
- 방제효과가 있다고 조사된 친환경농자재 6종에 대한 온실 검정 및 현장 적용 조건 도출
- 농가 실증을 통한 최적 처리 시기 및 처리 횟수 시험을 통해 환경 친화적 병 방제 체계 확립 및 방제 프로그램 개발

Abstract ▼

This study was performed Brown blight and Blister blight for Investigation Ecology occurrence on Tea tree. Research occurrence Ecology Investigation Hoecheon coast, Woongchi middle mountain, Boseong inland in three region. Occurrence Brown blight in Tea tree from May middle to October middle, The fi

This study was performed Brown blight and Blister blight for Investigation Ecology occurrence on Tea tree. Research occurrence Ecology Investigation Hoecheon coast, Woongchi middle mountain, Boseong inland in three region. Occurrence Brown blight in Tea tree from May middle to October middle, The first from July middle to August early and second from September early to September middle occurrence peak. seasonal prevalence regional occurrence August early and September early 10.3%, 16.7% each. Pathogen appear Brown blight on Tea tree is Collectotrichum gloeosporiodes and Glomerella cingulata, Collectotrichum gloeosporiodes the more Dominance. hypha growth each temperature fasted on 25℃and 30℃ after six day 8.3㎝, hyphal growth slowed 3.8㎝ on 15℃. Blister blight occurrence from June late to October early, ’08, ’09, ’10 year appeared occurrence peak late of disease leaf 3.2% of August middle, 3.7% of August late, 4.0% of September early. hypha growth of different temperatures in the 25℃ and 30℃ for seven day to 8.3㎝ 5.7㎝ was faster at 15℃ in a slow growth rate.
The present study was conducted to develop eco-friendly methods for controlling blister blight disease (pathogen: Exobasidium vexans) and brown blight disease (pathogen: Glomerella cingulata) of tea plants. Commercial products of biocontrol agents and natural substances were screened to test their antifungal activity against the pathogens. Among 10 biological agent products, bacterial isolates were present only from 5 commercial products. Among the 5 products, antifungal activity was only proved in three products of Ecozet, Biobong, and Zenotan. Eight natural substances showed very low antifungal activity at the recommended concentration. Quality control of the commercial products of biological agent was also tested. Generally the products contained the bacterial agents at the lower concentrations than the registration. Some products had different bacterial species from the registration. All the isolated biological agents were identified as Bacillus subtilus. The three products of Ecozet, Biobong, and Zenotan were found to be reliable and effective biological agents to control the disease. Endolichenic fungi were examined to develop new biological agents for the disease control. More than 200 endolichenic fungi were isolated from lichen thalli collected from Korean and Nepal Among them, seven fungal isolates showed strong antifungal activity against the pathogens. As a model fungus, NP20-4 isolate was selected due to red pigments which had a high antifungal activity of the fungus. The optimum culture conditions and antifungal substances were further examined with the fungal isolate. Malt-yeast extract medium was found to be the most effective one in terms of fungal growth and antifungal activity. The fungus also showed high growth rate and high antifungal activity at 25 oC for 1 week culture. Traditional shaking culture method was more effective in antifungal substance production than a novel submerged air floating culture system which allowed large scale culture with high mycelial biomass. The antifungal substances was partially purified from the red pigment fractions and their identification is now under investigation. The results suggested that few commercial products of biological agents can be registered to control foliar disease of tea plants. Novel antifungal substances can be feasible from endolichenic fungi which are considered as unexploited bioresources for agricultural application.
Commercial products of biocontrol agents were tested to evaluate control efficacy against blister blight disease and brown blight disease of tea plants in the field conditions in 2008 and 2009. Among the 18 products, only 2 products of Ecozet and Zenotan were effective in controlling the diseases. In 2010, total 5 products including Ecozet and Zenotan were tested at a local tea tree farm of Bosung area. Disease occurrence of blister blight disease and brown blight disease was recorded at 0.1% and 12.7% at the farm in 2009. Control efficacy of Ecozet and Zenotan was 48.2% and 45.1% at 4 applications and 53.5% and 49.8% at the six applications, respectively (DMRT 0.01). The first application dates of Ecozet were fixed at 15th, 22nd and 29th of July. Control efficacy was 48.2%, 48.0%, and 41.8%, respectively. The There was no difference in control efficacy among the first application dates (DMRT 0.05).
Control efficacy of Ecozet and Zenotan against blister blight disease was tested in a greenhouse with use of 2 year-old tea trees. Exobasidium vexans was cultivated on PAD plates for 2 weeks at 25℃ and inoculated on the tea leaves. Ecozet and Zenotan were applied 2 weeks later at 3 times with 7 day intervals. Control efficacy of Ecozet and Zenotan was 91.2% and 85.5%, respectively.

목차 Contents

• 제출문 ... 1
• 요약문 ... 2
• SUMMARY ... 6
• 제1장 서론 ... 8
• 제1절 연구의 필요성 ... 8
• 제2절 연구 목적 ... 9
• 제2장 국내외 기술개발 현황 ... 10
• 1. 국내 연구 현황 ... 10
• 2. 국외 연구 현황 ... 11
• 3. 국내외 연구현황 비교 및 필요 연구 분야 ... 12
• 제3장 연구개발수행 내용 및 결과 ... 13
• 제1절 차나무 잎마름병 및 떡병 발생생태 조사 ... 13
• 제2절 차나무 잎마름병 및 떡병에 대한 환경친화적 방제기술 개발 ... 20
• 제3절 차나무 잎마름병 및 떡병에 대한 환경친화적 방제체계 확립 ... 38
• 제4장 연구개발목표 달성도 및 대외기여도 ... 46
• 제1절 : 목표대비 대외달성도 ... 46
• 제2절 : 정량적 성과 ... 47
• 제5장 연구개발결과의 활용계획 ... 48
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 48
• 제7장 기타 중요 변동사항 ... 48
• 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 48
• 제9장 참고문헌 ... 49
• 주요 결과 요약서 ... 51