초록

- 전기자극에 의한 LTP 형성의 in vitro/vivo 확인
- 화학적 자극에 의한 LTP 형성을 in vitro/vivo로 확인
- 침지극으로 인한 진통효과와 LTP 감소의 확인
- UTMB로부터 LTP의 형성에 대한 뇌시상세포 및 세포막 변화 관찰 기술 이전
- UTMB로부터 침 가극에 대한 세포 내 LTP 형성 신호전달 변화 관찰 기수 이전

Abstract ▼

Ⅳ. Result of the research proposal
Chapter 1.
Study of LTP reduction generated by acupuncture treatment in electrical/chemical pain model
1. Study of LTP reduction generated by acupuncture treatment in electrical/chemical pain model
A. Induction of electronocal/chemical chronic pain and

Ⅳ. Result of the research proposal
Chapter 1.
Study of LTP reduction generated by acupuncture treatment in electrical/chemical pain model
1. Study of LTP reduction generated by acupuncture treatment in electrical/chemical pain model
A. Induction of electronocal/chemical chronic pain and long-term potentation(LTP) in the animal model
1) Induction of electrical stimulation generated chronic pain animal model
● As a result of study with four stimulation condition, we figured out that the condition of 2 Hz, 40 second and 100 Hz, 3 second was most effective for making chronic pain model.
2) Induction of LTP generated by electrical stimulation
● The result of patch clamp test in the STTn shows that all four kinds of electrical stimulation induces LTP for more than 30 min. And the stimulation condition of 300 pulses for 30 second induced LTP most significantly.
3) Induction of chemical stimulation generated chronic pain animal model
● Capsaicin (20ug/20ml) injection in the foot sole of rat induced hyperalgesia for more than 4 hours.
● NMDA (0.04ug/5ul saline) injection in the intervertebral space of mice induced hyperalgesia for more than 2 hours.
4) Induction of LTP generated by chemical stimulation
● As a result of patch clamp test in the spinal dorsal horn neuron cell, we could observe continues LTP 15 min after NMDA/ Glycine injection.
B. Confirmation of alleviation of pain and reduction of LTP as a result of acupuncture stimulation
1) Study on pain allevating effect of acupuncture treatment in chronic pain model
● We did acupuncture treatment on the combined acupuncture points(BL40+BL60, SI3+TE8, GV2+GV6, LI3+LI6 and GB30+GB34) of the capsaicin injected chronic pain model.
● Acupuncture treatment on the combination of GB30+GB34 and SI3+TE8 showed significant increase of pain threshold, and specially, SI3+TE8 showed great increasement.
2) LTP inhibiting of acupuncture in chemical stimulated chronic pain model
● Experiment using capsaicin-pain model is on going and will be end on may.
3) LTP inhibiting of acupuncture in electrical stimulated chronic pain model
● Experiment using electrical pain model is on going and will be end on may.
4) Study of inducing LTD by acupuncture treatment
● We finished setting of experimental instrument and protochol, and experiment will be end till August.
Chapter 2.
Establish of acupuncture-originated changes in cellular signal transmition for reduce pain
1. Establish of acupuncture-originated changes in cellular signal transmition for reduce pain
A. Observation of acupuncture stimuli generated changes in the synapse and cell membrane of the ventral posterolateral nucleus
1) Immunochemical assay of changes of VPN synapse under acupuncture treatment
● Professor Jung Jin-Mo in the UTMB developed the techiniques for observe changes in VPN synapse and VPN cell membrane.
● Professor Jung Jin-Mo will transfer the technique to Korean Institute of Oriental Medicine at August.
2) Study of mechanism in the LTP inducing cell membrane under acupuncture stimulation
● Professor Jung Jin-Mo in the UTMB developed the techiniques for observe changes in VPN synapse and VPN cell membrane.
● Professor Jung Jin-Mo will transfer the technique to Korean Institute of Oriental Medicine at August.
3) Developing and transferring techniques for observing changes of VPN synapse
● Professor Jung Jin-Mo in the UTMB developed the techiniques for observe changes in VPN synapse and VPN cell membrane.
● Professor Jung Jin-Mo will transfer the technique to Korean Institute of Oriental Medicine at August.
B. Observation of acupuncture stimuli related changes in thr signal transmission system of neuronal cell
1) Study on changes of signal transmission in VPN cell under acupuncture stimulation
● Professor Jung Jin-Mo in the UTMB developed the techiniques for observe changes in VPN synapse and VPN cell membrane.
● Professor Jung Jin-Mo will transfer the technique to Korean Institute of Oriental Medicine at August.
2) Study of neurotransmitter in the LTP inducing cell under acupuncture stimulation
● Professor Jung Jin-Mo in the UTMB developed the techiniques for observe changes in VPN synapse and VPN cell membrane.
● Professor Jung Jin-Mo will transfer the technique to Korean Institute of Oriental Medicine at August.
3) Developing and transferring techniques for observing signal transmission in VPN cell
● Professor Jung Jin-Mo in the UTMB developed the techiniques for observe changes in VPN synapse and VPN cell membrane.
● Professor Jung Jin-Mo will transfer the technique to Korean Institute of Oriental Medicine at August.

목차 Contents

• 표 지 ... 1
• 제 출 문 ... 3
• 보고서 초록 ... 7
• 요 약 문 ... 9
• 참 고 자 료 ... 25
• SUMMARY ... 29
• CONTENTS ... 35
• 목 차 ... 37
• 제1장 침의 만성통증 치료기전 ... 41
• 1. 침자극의 전기적/화학적 통증 및 LTP 형성 억제 연구 ... 43
• 1) 전기적/화학적 만성 통증 동물모델 확립 및 LTP 유발 ... 43
• 2) 침자극에 의한 진통효과 및 LTP 형성 억제 효과 연구 ... 48
• 2. 침자극에 의한 통증 억제 세포신호전달 변화 규명 ... 53
• 1) 침자극에 의한 뇌시상세포 시냅스, 세포막 변화 연구 ... 53
• 2) 침자극에 의한 세포내 신호전달 변화에 대한 연구 ... 56
• 참 고 문 헌 ... 63
• 끝페이지 ... 70