초록 ▼

연구개발결과
외국 도입품종에 대응하기 위한 우리 고유의 만숙성 감귤 신품종을 개발하고, 레몬 등 틈새 감귤류의 신품종을 개발하여 국민 보건증진과 농가소득 향상에 기여하고자 수행되어진 연구결과, 172조합에서 23,403개체가 육성 중이다. 만감류 16개체, 부지화 주심배실생 24개체 및 세토카 주심배실생 8개체가 유망 시 되었다. 레몬 교잡개체에서는 다양한 과형과 산함량이 높은 개체가 조사되었고, 금감 교잡개체 중에는 산함량이 일찍 낮아지는 개체가 조사되었다. 연구기간 동안 만감류에서 18개체가, 레몬류에서 1개체가 1차 선발

연구개발결과
외국 도입품종에 대응하기 위한 우리 고유의 만숙성 감귤 신품종을 개발하고, 레몬 등 틈새 감귤류의 신품종을 개발하여 국민 보건증진과 농가소득 향상에 기여하고자 수행되어진 연구결과, 172조합에서 23,403개체가 육성 중이다. 만감류 16개체, 부지화 주심배실생 24개체 및 세토카 주심배실생 8개체가 유망 시 되었다. 레몬 교잡개체에서는 다양한 과형과 산함량이 높은 개체가 조사되었고, 금감 교잡개체 중에는 산함량이 일찍 낮아지는 개체가 조사되었다. 연구기간 동안 만감류에서 18개체가, 레몬류에서 1개체가 1차 선발되었으며, 이 중 만감류 5개체는 직무육성신품종으로 선정되었다. 또한 9품종이 품종보호출원되었고, 1품종이 등록되었다.
더뎅이병에 대한 연구에서 7계통이 더뎅이병 저항성이 있었고, 검은점무늬병 저항성 계통은 8계통이 확인되었다. 조숙성이며 더뎅이병 및 검은점무늬병 저항성을 가진 품종개발을 위해 신규교배가 이루어졌다. 연구수행 중 붉은 과피를 가진 2계통을 선발하였고, 1계통을 품종 출원을 하였으며 3계통을 선발하였다. 온주밀감 주심배실생 중에서 조생계 5계통을 선발하였다. 또한 온주밀감(궁천조생)보다 나린진의 함량이 1.5～2배 많이 함유된 하밀감을 품종 출원하였으며, 12월 중하순에 성숙기를 가지며 당도가 12°Bx 내외이고 산함량이 1.0% 이하로 품질이 우수한 신예감을 품종 출원하였다.
감귤에 있어 원형질체 융합에 의한 식물체 생산을 위해서 총 264개체의 체세포 잡종체를 생산하였고 배수성 검정 결과 92개체는 4배체였고 나머지 172개체는 2배체 식물체였다. 또한 69개체가 이종간 원형질 융합 식물체로 판단되었다.
감귤 데뎅이병 저항성 마커 개발을 위해 11개의 프라이머 세트를 확보하였고, 하례조생 잎의 RNA로부터 EST 데이터를 확보하였다. 가뭄저항성 유전자 등 4개는 클로닝을 완료하였고 나머지 5개의 유전자에 대한 클로닝을 실시하고 있다. 하례조생 EST를 바탕으로 SSR 분석을 실시하여 578개의 SSR을 확인하였고 312개의 프라이머를 작성하였다. SSR 프라이머를 이용하여 9품종에서 17개의 품종 특이 마커를 선발하였다. 하례조생과 궁천조생의 판별 마커 HR026, HR151, HR182를 선발하였다.

Abstract ▼

This study of “Development of new citrus varieties and breeding resources” was divided into four areas was performed, results are as follows;
1. First study was carried out to develop late maturing domestic varieties of citrus to substitute the introduction of foreign citrus varieties and breed l

This study of “Development of new citrus varieties and breeding resources” was divided into four areas was performed, results are as follows;
1. First study was carried out to develop late maturing domestic varieties of citrus to substitute the introduction of foreign citrus varieties and breed like lemon and kumquat. Results obtained in yers of hand pollination have been depicted. From years 2009-2012 there were 11,403 individuals cross pollinated from which 76 cross combinations were obtained. Similarly, there were 12,030 individuals from 96 cross combination including nucellar seedling of ‘Shiranuhi’ and ‘Setoka’ growing as the result of cross during 2003-2008. In 2013, 2,736 individuals of 8 cross combination obtained. Seeds were sown and seedlings were grafted efficiently before July. Total 16 individuals of late maturing varieties, 24 nucellar seedling of ‘Shiranuhi’ and 8 individual of ‘Setoka’ developed are growing. Among the crossed lines, lemon crossed showed morphological variations in fruits. Fruits of kumquat crossed varieties showed declination of acidity earlier than others. Primarily, there are 18 individuals of late maturing citrus and one individual of lemon were selected for cultivation. Among them 5 individuals of late maturing type are listed as new varieties. There are 5 late maturing new varieties and 2 parental lines developed are applied for a variety protection. One variety has been registered by Korea seed & variety service.
2. To investigate the degree of citrus scab-resistance, leaves were inoculated with scab pathogens and investigated the damage. As a result, CG-181, KG-431, KW-132, KG-238, CG-04, WG-06, KG-03, strains was confirmed to have a scab-resistance. To selectmelanose-resistance strains, total 48 citrus strains were investigated. CG-04, KW-61, Mip-9, Mip-3, ML-14, KG-238, KG-431, ‘Shinyegam’, such strains was confirmed to have a good melanose-resistance. To breed citrus cultivar having early maturinng trait, scab-resistance and Melanose-resistance, 55 combination crossed of kiyomi(C. unshiu × C. sinensis), ‘Morita unshiu’(C. unshiu Marc.), etc and 4392 seedlings were obtained during 5years. To evaluate the fruit characteristics of 58 seedling stains, ‘roseok-72’ and ‘riseok-89’, with the red rind were selected in 2011, 02-C-KW-358 was selected by high-quality fruit in 2012 and variety protection application was complete in 2013. KW-61 was selected by high-quality fruit, good fruit shape, beautiful rind color, 05-C-ML-14 and 05-C-ML-19 was selected by early colored. 27 strains of the seedling of Ueno wase nucellar were compared with ‘Ueno wase’(C. unshiu Marc.), ‘Iwasaki’(C. unshiu Marc.), ‘Nichinan NO.1’(C. unshiu Marc.), were selected 5 strains have excellent fruit quality. They will apply for variety protection in 2014. In this study, ‘Hamilgam’ was rich naringin content more than 1.5 to 2 times than Miyakawa wase(C. unshiu Marc.), and ‘Shinyegam’ ripened the middle, last part of deccember, and the sugar content is around 12 ° Bx and acid content of 1.0% or less, so, they applyed for variety protection.
3. In citrus, in order to produce protoplast fusion plants, first it is necessary to make the callus protoplast and plant protoplast. So experimented to induced citrus callus through ovule culture. As a result, most of citrus system, higher number of callus were induced in early collections. And callus induction from different varieties showed variable result in different medium. Between the each system, conducted a protoplast fusion using produced in vitro callus and plant. 264 plantlet were re generated by protoplast fusion. Ploid analysis results showed that 92 individuals are tetraploid and 172 individuals are diploid. These 92 tetraploid protoplast fusion plants using SSR primer checked whether plants are fusion or not. As a result, 69 plantlet were allotetraploid. For the future, the protoplast fusion plants will also be used as parents for seedless triploid breeding program.
4. To develop markers linked to citrus-carb resistance, sequence-related amplification polymorphism(SRAP) combined with bulked segregant analysis. From the profiling SRAP primer sets, 11 primer sets were closely inked to citrus-carb resistance trait. The EST(expressed sequence tag) data was obtained from ' Haryerjosaeng' leaf RNA, that was conducted GO analysis and useful gene groups were identified. Nine important genes of the identified were selected, such as genes related to drought tolerance, fruit color changes, flowering time and salt stress were cloned complete cloning and remaining 5 genes are conducted cloning. Base on ' Haryerjosaeng' EST data, SSR analysis was performed and 578 SSR region were confirmed. Using them, 312 SSR primer sets constructed. SSR primers were used to identify genetic discrimination among 24 kind of citrus cultivar, 17 cultivar-specific markers were selected in 9 cultivars. Difficulty to determine ' Haryerjosaeng' and miyagawa wase by Phenotype was overcame by using SSR primer sets, and dentify genetic discrimination marker HR026, HR151, HR182 were selected.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 7
• 목차 ... 9
• 제 1 장 서 론 ... 10
• 제 2 장 국내외 기술개발 현황 ... 12
• 제 3 장 연구개발수행 내용 및 결과 ... 14
• 1절 이론적ㆍ실험적 접근방법 ... 14
• 2절 연구내용 ... 15
• 3절 연구결과 ... 18
• 제 4 장 연구개발목표 달성도 및 대외기여도 ... 75
• 1절 목표대비 대외 달성도 ... 75
• 2절 정량적 성과 ... 76
• 제 5 장 연구개발결과의 활용계획 ... 78
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 79
• 제 7 장 기타 중요 변동사항 ... 80
• 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 81
• 제 9 장 참고문헌 ... 82
• 끝페이지 ... 83