보고서 정보
주관연구기관 |
국립축산과학원 National Institute of Animal Science |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2014-03 |
과제시작연도 |
2013 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201400011076 |
과제고유번호 |
1395030421 |
사업명 |
국책기술개발 |
DB 구축일자 |
2014-07-05
|
키워드 |
Foot and Mouth Disease (FMD).FMD SOP(Standard Operating Procedure.Korea).NaOH treatment.Citric acid neutralization.Thermophilic Aerobic Oxidation (TAO) system.
|
DOI |
https://doi.org/10.23000/TRKO201400011076 |
초록
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Ⅳ. 연구개발결과
본 연구를 통해서 달성한 연구결과는 다음과 같다
1) 가축분뇨 퇴비화에 따른 구제역, 조류독감 유사 병원성균 사멸효율 평가 결과를 도출하였다.
2) 가축분뇨 액비화에 따른 구제역 유사 병원성균 사멸효율 평가 결과를 도출하였다
3) 구제역 기급행동지침(SOP) 상의 산·알칼리처리가 양돈분뇨의 이화학적 성상에 미치는 영향을 구명하였다
4) 구제역 긴급행동지침(SOP) 상의 화학적 처리방법과 양돈분뇨의 고온발효소독장치 적용효율을 구명하였다
5) 가축분뇨 처리방법에 따른 EMCV(구제역 유
Ⅳ. 연구개발결과
본 연구를 통해서 달성한 연구결과는 다음과 같다
1) 가축분뇨 퇴비화에 따른 구제역, 조류독감 유사 병원성균 사멸효율 평가 결과를 도출하였다.
2) 가축분뇨 액비화에 따른 구제역 유사 병원성균 사멸효율 평가 결과를 도출하였다
3) 구제역 기급행동지침(SOP) 상의 산·알칼리처리가 양돈분뇨의 이화학적 성상에 미치는 영향을 구명하였다
4) 구제역 긴급행동지침(SOP) 상의 화학적 처리방법과 양돈분뇨의 고온발효소독장치 적용효율을 구명하였다
5) 가축분뇨 처리방법에 따른 EMCV(구제역 유사바이러스)의 불활성화 효율을 평가하였다.
6) 고온발효 액상물을 이용한 알칼리 고형화 장치 및 하수정화방류 연계 기술적용효과를 분석하였다.
Abstract
▼
This study was conducted to test whether pathogenic viruses such as EMCV or H9N2 were annihilated by the animal manure composting process or the fermenting process of slurry. Manures of cow, pig, and chicken were used for composting process and pig slurry was used for the fermentation of slurry. To
This study was conducted to test whether pathogenic viruses such as EMCV or H9N2 were annihilated by the animal manure composting process or the fermenting process of slurry. Manures of cow, pig, and chicken were used for composting process and pig slurry was used for the fermentation of slurry. To analyze the extinction rate, viruses were place inside a dialysis cassette and cultured in a specified media. In case of manure composting, the highest temperature of compost was up to 76℃. After the completion of treatment for pre-determined time interval, extinction rate of virus has analyzed. Results can be summarized as these; (1) When activity of viruses (EMCV and H9N2) buried in manure compost were compared, H9N2 appears to be more sensitive than EMCV, meaning EMCV has higher durability rate. (2) when EMCV was buried in both manure compost and fermented pig slurry, the one in manure compost showed weaker survival. Overall, we could conclude that the survival of pathogenic viruses were much less likely in both manure compost and fermented pig slurry.
Foot and Mouth Disease (FMD) is one of a highly communicable disease among the infectious diseases in domestic animals. Office International des Epizooties (OIE) classifies it as the first class communicable diseases. Disinfecting contaminated swine manure with FMD(Foot-and-Mouth Disease) and pathogenic viruses is very important for maintaining sanitized environment, however, rare research reported on this subject, especially for post-disinfection to utilize the wastes as a renewable resource. This research is carried out to obtain basic information for chemical treatment in FMD SOP(Standard Operating Procedure, Korea) of contaminated swine manure. Using lime, sodium hydroxide, citric acid and hydrochloric acid, described in FMD SOP, the effects of chemical treatments on livestock manure were compared in this paper. Four combinations of alkali-acid treatments and four kinds of acid-alkali combinations were tested to find out effective method. Total coliform bacteria in contaminated swine manure, 2.5×104CFU/㎖, decreased to the range of 1/1000~1/100 in all treatments. Some specific disinfectants increases SCOD(Soluble Chemical Oxygen Demand) and EC(Electric Conductivity), especially, alkaline treatments increases ammonia level than in case of acid treatments. These findings suggest that the treatment methods should be considered as an important environmental factor in post-disinfection of contaminated animal manure with pathogenic microorganisms.
According to Korean Standard Operating Procedure(SOP), the infected excrements by FMD must be disinfected to a pH above 10 using alkaline chemicals. Then, leave it for about 2-3 days. After confirming that the pH has elevated above 10, acidic chemicals can be used in the infected excrements to neutralize pH to 8. After that, the neutralized excrements can be disposed in accordance with Livestock Manure Management Scheme. We planned to study how the chemical disposal designed by SOP can affect physico-chemical compositions of the livestock excrement. According to Livestock Manure Management Scheme, we experimented in two steps; the first step, NaOH treatment-Citric acid neutralization, and then the second procedure, Thermophilic Aerobic Oxidation (TAO) system. Physicochemical compositions of the domestic swine excrements in the experiments showed pH 7.55, EC 23.5mS/cm, ORP -303.4mV, SCOD 2,461mg/L, T-N 5,466mg/L, NH4+-N 2,469mg/L, E. Coli. 2.2x104CFU/mL. Physicochemical compositions of the 3-days-old samples after NaOH treatment were pH 10.31, EC 24.54mS/cm, ORP -379.3mV, SCOD 3,022mg/L, T-N 4,315mg/L, NH4+-N 1,960mg/L, and not detected E. coli.. And those of one-day-old samples after citric acid neutralization were pH 7.36, EC 32.89mS/cm, ORP -243.8mV, SCOD 12,733mg/L, T-N 4,787mg/L, NH4+-N 2,450mg/L, and E. coli. not detected. In contrast, the physicochemical compositions of the treatment plots after the second treatment with TAO system(72hr) were pH 9.42 EC 24.21mS/cm, ORP -22.7mV, SCOD 3,660mg/L, T-N 3,616mg/L, NH4+-N 1,190mg/L, and no detection of E. coli.. The second treatment showed that the temperature increased up to 60℃, the typical characteristics of TAO. It also showed the tendency that the inner temperature has gradually raised for the first 20 hours after the treatment, and maintained constant at above 50℃ for another 20 hours, then gradually decreased after 40 hours. The result of this research indicates that the second treatment (TAO) followed by the first treatment(with NaOH) make it possible to aerobic fermentation of swine excrement samples and to decrease in the concentration of pollutant.
목차 Contents
- 표지 ... 1
- 제출문 ... 2
- 요약문 ... 4
- SUMMARY ... 7
- 목차 ... 9
- 제 1 장 서 론 ... 10
- 제 2 장 국내외 기술개발 현황 ... 13
- (1) 국내 연구 현황 ... 13
- (2) 국외 연구 현황 ... 14
- 제 3 장 연구개발수행 내용 및 결과 ... 17
- < 제1세부과제 : 가축분뇨 처리 유형별 병원성균 사멸효과 평가 및 개선기술 개발> ... 17
- <제2세부과제 : 병원성바이러스 오염 가축분뇨 처리 기술 개발> ... 38
- 제 4 장 연구개발목표 달성도 및 대외 기여도 ... 64
- 1절 : 목표대비 달성도 ... 64
- 2절 : 정량적 성과 ... 66
- 제 5 장 연구개발결과의 활용계획 ... 68
- 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 69
- 제 7 장 기타 중요 변동사항 ... 74
- 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 75
- 제 9 장 참고문헌 ... 76
- 끝페이지 ... 79
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