초록 ▼

o 연구결과
· 동물모델에서 40종 생약이 염증 및 천식억제작용이 있는지 검색하여 약효를 갖는 8종의 생약의 chromatography를 통하여 약 45종 성분을 단리하여 화학구조를 규명하였고, 그중 16개 성분의 항염 또는 항천식 효능을 검색한 결과, 약효성분 10개을 창출하였다.
· 길경에서 단리한 platycodin D 및 D₃가 36 및 24mg/kg 투여군에서 항염작용이 있었다.
· 독활의 에텔분획에서 항천식작용을 갖는 약효성분인 3종의 pimaradienes 를 단리하였으며, 그 중 7

o 연구결과
· 동물모델에서 40종 생약이 염증 및 천식억제작용이 있는지 검색하여 약효를 갖는 8종의 생약의 chromatography를 통하여 약 45종 성분을 단리하여 화학구조를 규명하였고, 그중 16개 성분의 항염 또는 항천식 효능을 검색한 결과, 약효성분 10개을 창출하였다.
· 길경에서 단리한 platycodin D 및 D₃가 36 및 24mg/kg 투여군에서 항염작용이 있었다.
· 독활의 에텔분획에서 항천식작용을 갖는 약효성분인 3종의 pimaradienes 를 단리하였으며, 그 중 7-0xo-8(14), 15-pimaradiene-19-oic acid가 천식 초·후기에 강한 약효물질이었다 또 천마에서 1,2-Bis [4-(β-D-glucopyranosyloxy) benzyI] citrate, 골담초에서 acacetin-7-O-β-Dglucopyranoside, 호대황에서 1-O-ferruloyl glucoside, 호대황에서 quercetin-3-O-β-D-glucuronopyranoside, 적승마에서 bergenin, 남부오갈피에서 quercetin-3-O-galactoside, 누리 장나무에 서 3,4-dihydroxy-5-α-L-rhamnopyranosyl-(1,3)-6-5-caffeoyl-5-β-glucopyrano side가 각 생약의 약효성분이었으며, 이들은 50mg/kg 투여로 항천식작용이 있었다.

Abstract ▼

To develope the new anti-inflammatory and anti-asthmatic agent from medicinal plants, activity-guided separation from methanol extract of seven chinese medicines were carried out by chromatography with Amberlite XAD-2. Anti-inflammatory activity were studied in carrageenin-induced paw edema, capilla

To develope the new anti-inflammatory and anti-asthmatic agent from medicinal plants, activity-guided separation from methanol extract of seven chinese medicines were carried out by chromatography with Amberlite XAD-2. Anti-inflammatory activity were studied in carrageenin-induced paw edema, capillary permeability and white cell infiltration by air pouch method. Ovalbumin-sensitized guinea pigs were placed in the double-chambered plethysmograph (HSE type 855) consisting of a nasal chamber(head) and a thoracic chambedbody). Conscious animals were challenged in the head chamber by the inhalation of aerosolized-ovalbumin. The specific airway resistance (sRaw), tidal volume(TV) and respiration rate in immediate asthmatic response (lAR) and late-phase asthmatic response (LAR) for screening of anti-asthmatic activity were determinded by Pennock program as compared with salbutamol, cromoglycate and dexamethasone. Twenty four hours after antigen challenge, the lungs were lavaged with 5ml saline 6 times and this bronchoalveolar lavage fluid (BALF) was centrifuged at 1000xg for lOmin at 4℃. The cell pellet was resuspened in 5ml HEPES buffer. Slides were prepared by cytospin, and differential cell counts were done after Wright's stain. Histamine, protein and phospholipase A₂ were analyzed in the aliquot of BALF. Histological study was carried out after exsanguination of guinea pigs.
By the activity-guided separation of eight medicinal plants, 45 biologically active agents were isolated by chromatography. From the screen test of anti-asthmatic activity of fifteen compounds, ten biologically active agents have significant anti-inflammatory or anti-asthmatic activity. Platycodin D₃ and D isolated from Platycocli Radix have significant antiinflammatory activity at a oral close of 24 and 36 mg/kg, respectively. Three diterpenes isolated from ether fraction of Aralis cordatae root have significant anti-asthmatic activity, especially, 7-oxo-8 (4), 15-pimaradiene-19-oic acid at a oral dose of 25mg/kg inhibited significantly not only the immediate and late-phase asthmatic responses, but also the recruitment of eosinophils into the lung, and exudative protein and phospholipase A₂ activity in BALF. And also 1,2-bis [4-(β-D-glucopyranosyloxy) benzyl] citrate from Gastrodiae Rhizoma. acacetin-7-O-β-D-glucopyranoside from Caragana chamlagu leaf, l-O-ferruloyl glucoside from aerial part of Rumex gmelini, quercetin-3-O-β-D-glucuronopyranoside from Rumex aquaticus, bergenin from Astilbe macrophylla root, quercetin-3-O-galactoside from Acanthopanax nambunensis, and 3,4-dihydroxy-5-α-L-rhamnopyranosyl-(1,3)-6-5-caffeoyl-5-β-glucopyranoside from Clerodendron trichotomum leaf, have significant anti-asthmatic activity in IAR and LAR. All of this active agents inhibited not only the specific airway resistance at IAR and LAR, but also recruitment of leukocytes, especially eosinophils, protein. histamine and phospholipase Az_ in BALF at LAR. It showed that anti-asthmatic agent originating from medicinal plants inhibits the recruitment of eosinophils into the lungs and infiltration of leukocytes around venules.

목차 Contents

• 표 지 ... 1
• 연구개발 최종보고서 ... 3
• 제 출 문 ... 4
• 요 약 문 ... 5
• 영 문 요 약 문 ... 6
• 목 차 ... 7
• 제 1 장 서론 ... 9
• 제 1 절 연구개발의 목적 ... 9
• 제 2 절 연구개발의 필요성 ... 10
• 제 3 절 연구개발의 범위 ... 11
• 제 2 장 국내·외 기술개발 현황 ... 12
• 제 1 절 국외 연구개발 현황 ... 12
• 제 2 절 국내 연구개발 현황 ... 15
• 제 3 장 연구개발수행 내용 및 결과 ... 17
• 제 1 절 제 1 세부과제 ... 17
• 제 2 절 제 2 세부과제 ... 59
• 제 3 절 제 3 세부과제 ... 76
• 제 4 장 연구개발목표 달성도 및 대외기여도 ... 116
• 제 5 장 연구개발 결과의 활용성과 및 계획 ... 117
• 제 6 장 기타 중요변경사항 ... 118
• 제 7 장 참고문헌 ... 119
• 최종보고서 요약 (초록) ... 135
• 끝페이지 ... 135