초록 ▼

개발내용 및 결과
- 바이오매스 폐자원 당화액내 발효저해산물 무독화 방안 도출
(계면활성제 첨가와 전기화학적 제독 방법을 이용하여 강력한 무독화 방안도출)
- 포도당과 목당 동시 이용 균주개발
- Random mutation과 metabolic engineering을 통한 유기산 생산 수율 및 속도 향상 기술 개발
- Clostridia에서 metabolic engineering을 시행할 수 있는 기법정착
- NTG, gamma-ray를 이용한 돌연변이 유발을 통하여 발효저해물질 및 최종대사산물에 내

개발내용 및 결과
- 바이오매스 폐자원 당화액내 발효저해산물 무독화 방안 도출
(계면활성제 첨가와 전기화학적 제독 방법을 이용하여 강력한 무독화 방안도출)
- 포도당과 목당 동시 이용 균주개발
- Random mutation과 metabolic engineering을 통한 유기산 생산 수율 및 속도 향상 기술 개발
- Clostridia에서 metabolic engineering을 시행할 수 있는 기법정착
- NTG, gamma-ray를 이용한 돌연변이 유발을 통하여 발효저해물질 및 최종대사산물에 내성을 갖는 돌연변이 균주 확보
- 고농도 유기산 생산의 최적 운전조건 도출을 위한 RSM 기법 개발 및 최적화
- 나노촉매 설계 및 제작(신규 촉매 3종 이상 합성)
- 촉매활성 증진제 도입에 따른 알콜 수율 향상
- 수분 내성 및 비활성화 지연기술 도출
- 신규 촉매에서의 descriptor 측정 및 평가
- 촉매 descriptor의 검증
- 이상의 개발로 상기 최종목표치 초과 달성

Abstract ▼

Ⅳ. Results of R&D
○ Production of high concentrated organic acid using lignocellulosic hydrolysate
▸ Development of detoxification process
Phenolic compounds generated from lignocellulsic biomass during pretreatment and hydrolysis process inhibit the growth of Clostridia so that the final c

Ⅳ. Results of R&D
○ Production of high concentrated organic acid using lignocellulosic hydrolysate
▸ Development of detoxification process
Phenolic compounds generated from lignocellulsic biomass during pretreatment and hydrolysis process inhibit the growth of Clostridia so that the final concentration of fine metabolites . Here, we evaluated effects of p-coumaric acid, ferulic acid, vanillin, syringaldehyde, and soluble lignin as model phenolics on the growth and butyric acid production of Clostridium tyrobutyricum ATCC 25755, and developed a simple and inexpensive method adding Tween 80 to the culture or electrochemical treatment to eliminate the inhibition property of phenolics. When this process applied hydrolysate, we confirm powerful detoxification effect.
▸ Development of strain which was capable of utilizing glucose and xylose simultaneously
Clostridium tyrobutyricum ATCC 25755 is one of the most representative bioproducers for butyric acid. This strain was capable of utilizing glucose and xylose simultaneously. However, the xylose utilization rate was much lower than the glucose utilization rate and there was a diauxie in the mixed sugars due to glucose repression. Consequently, the final butyric acid productivity was lower in the mixture than in glucose. In this study, we developed an adaptive cultivation system to improve the xylose utilization rate and to release the glucose repression.In conclusion, we successfully developed an adaptive and regenerative cultivation system to improve the productivity of butyric acid by C. tyrobutyricum from glucose and xylose mixture by improving xylose consumption rate and releasing diauxie.
▸ Development of high concentrated organic acid tolerant strain
End product inhibition is the main problem that faces butyric acid fermentations and different approaches are being tried to improve the tolerance of Clostridium strains to the produced butyric acid. The addition of NaOH to neutralize produced butyric acid is a conventional operation thus sodium butyrate was accumulated. In this study, we describe the use of NTG mutagenesis for improved sulfate tolerance by C. tyrobutyricum ATCC 25755. After three rounds of NTG mutation, one strains that could grow in presence of 30 g/L of sodium butyrate were obtained.
m Alcohol conversion of organic acid using nano-catalyst
▸ Development of optimum catalyst
For hydrogenolysis of butyl butyrate (BB), a series of Cu/ZnO/Al2O3 catalysts with different metal compositions were prepared. As a result, the presence of ZnO in Cu-based catalysts was found to enhance the catalytic activity of Cu due to dual function of ZnO. The Cu surface area was linearly correlated with the butanol productivity, demonstrating that ZnO exerts the structural function in Cu/ZnO/Al2O3 catalysts. Additionally, the role of ZnO as a chemical contributor was revealed such that its presence leads to lower activation energy of the surface reaction, thus resulting in higher Cu catalytic activity obtained at a low temperature such as 200℃. Consequently, optimizing the Cu/Zn ratio in Cu/ZnO/Al2O3 catalyst is required to tune its structural and chemical characteristics of Cu metals, and thus to obtain a higher activity on the hydrogenolysis reaction.

목차 Contents

• 제출문 ... 1
• 보고서 초록 ... 2
• 요약문 ... 5
• SUMMARY ... 8
• 목차 ... 12
• 표 목차 ... 14
• 그림 목차 ... 16
• 제1장 서론 ... 22
• 제1절 연구개발의 중요성 및 필요성 ... 22
• 제2절 연구개발의 국내외 현황 ... 24
• 제3절 연구개발대상 기술의 차별성 ... 44
• 제2장 연구개발의 목표 및 내용 ... 49
• 제1절 연구의 최종목표 ... 49
• 제2절 연도별 연구개발의 목표 및 평가방법 ... 50
• 제3절 연도별 추진체계 ... 51
• 제3장 연구개발 결과 및 활용계획 ... 55
• 제1절 연구개발 결과 및 토의 ... 55
• 제2절 연구개발 결과 요약 ... 256
• 제3절 연도별 연구개발목표의 달성도 ... 258
• 제4절 연도별 연구성과 ... 259
• 제5절 관련분야의 기술발전 기여도 ... 259
• 제6절 연구개발 결과의 활용개획 ... 261
• 제4장 참고문헌 ... 262
• 부록 ... 270
• 끝페이지 ... 278