초록 ▼

○ 연구결과
(1) 흑회색 느타리버섯을 이용한 고품질 품종 개량에의 연구
·제작된 교잡균주들의 갓색 관련 이화학적 분석과 재배특성을 통한 6개 우량균주를 선발함
·프로테옴 분석을 통한 색변이주의 6개 특이적 발현 단백질을 동정함
·모균주와 선발 교잡균주간 DNA 다형성의 특성차이를 분석함
(2) 저온성 느타리버섯의 육종소재 개발 연구
·저온성에 강하고 자실체 수량이 많은 2개 우수 교잡균주를 최종적으로 선발함
·교배모본과 육성 단핵균주에 대한 DNA profile을 작성함
(3) 내병성 느

○ 연구결과
(1) 흑회색 느타리버섯을 이용한 고품질 품종 개량에의 연구
·제작된 교잡균주들의 갓색 관련 이화학적 분석과 재배특성을 통한 6개 우량균주를 선발함
·프로테옴 분석을 통한 색변이주의 6개 특이적 발현 단백질을 동정함
·모균주와 선발 교잡균주간 DNA 다형성의 특성차이를 분석함
(2) 저온성 느타리버섯의 육종소재 개발 연구
·저온성에 강하고 자실체 수량이 많은 2개 우수 교잡균주를 최종적으로 선발함
·교배모본과 육성 단핵균주에 대한 DNA profile을 작성함
(3) 내병성 느타리버섯의 육종소재 개발 연구
·자실체 생산성 및 병 저항성이 우수한 1개의 교잡균주(CBNU 925-97)를 최종적으로 선발함
·느타리버섯의 세균성갈색무늬병에 대한 저항성 측정에 적합한 생물검정법 정립
·교잡균주의 유전양식 분석 결과 세균성갈색무늬병이 다인자적형질(QTL)에 의해 조절됨을 확인함

Abstract ▼

Various strains of edible and medicinal mushroom are cultivated all over the world. Of these mushrooms, Pleurotus ostreatus known as oyster mushroom is one of the most widely cultivated edible mushrooms. During recent years, the world production of oyster mushroom has increased tremendously and now

Various strains of edible and medicinal mushroom are cultivated all over the world. Of these mushrooms, Pleurotus ostreatus known as oyster mushroom is one of the most widely cultivated edible mushrooms. During recent years, the world production of oyster mushroom has increased tremendously and now is ranked second to the button mushroom. P. ostreatus is especially appreciated in Asia including Korea for its edibility. The fruiting bodies are highly prized because of its delicious flavor and it has a high dietetic quality because of their high protein and vitamin content and its important level of unsaturated fatty acids. It is an important products in low calorie diets. The protein content of P. ostreatus is important and higher than in most of plants, excluding some pulse plants. But, new strains of fruiting body in P. ostreatus are still poorly developed. Also, the molecular mechanism of fruiting body in P. ostreatus is still poorly understood. The objectives of this study are to develop new strains for color trait, cold tolerance or disease resistance in P. ostreatus.
1. Development of new strain for pileus color trait in Pleurotus ostreatus.
The pileus color of fruiting bodies may be one of the important traits for strain improvement of mushrooms. To develop new strain for color trait, black mutant strain and ASI2504(Suhan) strain were selected as parental strains for crossing. The cross combination of black mutant strain and ASI2504(Suhan) strain producted 333 combination strains. Mushroom pileus color was evaluated by using UV/VIS spectrometer and colorimeter. The texture property of fruiting body was tested by the texture analyzer. Cultivating characteristics of 333 combination strains were tested from the cultivation with the plastic tray and the bottle, and the color density of pileus was measured. Six combination strains were selected under the basis of color and productivity traits. Specially KU-BM12×SM2 is supposed to be registered as a practical cultivar after actual practice in the farm. For the molecular study of color difference, we compared the expression profiles of mushroom proteome between two mutants of black and white color pileus by two-dimensional electrophoresis. More than 700 protein spots were detected on each gel. We found that 6 spots were expressed differently among different colored strains. These selected 6 spots were identified as two unknown proteins, ATP synthase, enolase, manganese superoxide dismutase and cyclophilin. It must be studied about the relationship between differently expressed proteomes and color difference of mushrooms.
2. Development of new strain for cold tolerance in Pleurotus ostreatus.
To develop a new superior strain, eight strains passed an examination of cultivation characteristics of collected commercial strains were selected as parental strains for crossing. Characteristics of 622 crossed dikaryotic strains derived from four cross combination in the second year and seven in the third year were tested in the bottle and plastic box cultivation. In the third year, selected strains and developed dikaryotic strains were tested again and finally one strain in each crossing combination was selected in the natural condition of low temperature (below 12℃) with the box cultivation which use just like same substrate to the mushroom farm in the winter. Final selected strains must have the tolerance to the low temperature. Even though there are some variations in their superior characteristics according to their combination, selected ones in each combination deserve to be commercial strains or breeding materials. Specially KU-254 from ASI2183 x ASI2344 and KU-415 from ASI2183 x ASI2706 are supposed to be registered as a practical cultivar after actual practice in the farm. We have a plan to expand productivity test for cultivar registration to the mushroom farm in association with corresponding company.
3. Development of new strain for disease resistance in Pleurotus ostreatus.
The brown blotch disease causes significant loss of cultivated mushroom crops. This is mainly due to Pseudomonas tolaasii. To develop new strain resistant to brown blotch disease, we firstly developed photoshop index which is calculated from digital camera and photoshop program for the rapid and accurate screening against the disease detection. The optimal conditions for photoshop index were examined, and the index values were compared with the results of eye index. Using the eye index and photoshop index, we selected the resistant strains and the sensitive strains to brown blotch disease as parental strains for crossing from the collected commercial strains of Pleurotus species. The distribution pattern of disease severity was examined from the mating strains of CBNU9(resistant strain) and CBNU25(sensitine strain). The result with normal distribution curve suggested that the disease-resistant traits may be regulated by multifactors, not single factor. For screening superior strains resistant to brown blotch disease, two strains of CBNU9 and CBNU25 were crossed and the CBNU9 25-97 strain showing the most disease resistance and good productivity was selected. This CBNU9 25-97 strain is supposed to be registered as a practical cultivar after actual practice in the farm.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 7
• CONTENTS ... 10
• 목차 ... 11
• 제 1 장 연구개발 과제의 개요 ... 12
• 1. 연구개발의 목적 ... 12
• 2. 연구개발의 필요성 ... 12
• 3. 연구개발의 범위 ... 14
• 제 2 장 국내외 기술개발 현황 ... 17
• ○ 국외 연구동향 ... 17
• ○ 국내 연구동향 ... 18
• 제 3 장 연구개발 수행내용 및 결과 ... 20
• 제 1 절. 흑회색 느타리버섯의 육종소재 개발 연구 ... 20
• 제 2 절. 저온성 느타리버섯의 육종소재 개발 연구 ... 64
• 제 3 절. 내병성 느타리버섯의 육종소재 개발 연구 ... 110
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 141
• 제 1 절. 흑회색 느타리버섯을 이용한 고품질 품종 개량에의 연구 ... 141
• 제 2 절. 저온성 느타리버섯의 육종소재 개발 연구 ... 144
• 제 3 절. 내병성 느타리버섯의 육종소재 개발 연구 ... 145
• 제 5 장 연구개발결과의 활용계획 ... 147
• 제 1 절. 흑회색 느타리버섯을 이용한 고품질 품종 개량에의 연구 ... 147
• 제 2 절. 저온성 느타리버섯의 육종소재 개발 연구 ... 147
• 제 3 절. 내병성 느타리버섯의 육종소재 개발 연구 ... 148
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 149
• 1. Repetitive sequence로부터 제작된 universal rice primer ... 149
• 2. 숲속의 버섯에서 나는 강력한 항균물질 ... 149
• 3. 버섯균으로 다이옥신 분해하는 기술 ... 150
• 4. 단백질 칩 기술 ... 151
• 5. 감자(Solanum tuberosum L.)에 Chitinase 내병성유전자의 도입 및 발현 ... 151
• 제 7 장 참고문헌 ... 152
• 끝페이지 ... 154