초록

ㅇ 연구결과
- 농진청에서 인출한 나물콩 유전자원으로부터 1,139개의 순계를 만들고 이에 대한 농업적 및 콩나물의 특성검정을 하여 data base화
- 숙취해독 제제의 개발
- 천연자원으로부터 생장조정 효능이 있는 물질의 개발
- 콩나물로부터 아스파라긴과 이소플라본을 동시에 경제적으로 추출하는 방법개발

Abstract ▼

1. Development of high functional soy-sprout varieties and merchandises
A. Much more greater variations than expected were observed in the important agronomic and sprout characteristics from those 1,139 indigenous soybean sprout lines which were planted on the same date this year after developmen

1. Development of high functional soy-sprout varieties and merchandises
A. Much more greater variations than expected were observed in the important agronomic and sprout characteristics from those 1,139 indigenous soybean sprout lines which were planted on the same date this year after development as pure inbred lines in the last two years. For examples, the ranges of days to flower and to maturity were 35∼85 and 85∼152, respectively, which could successfully be used for the development of new sprout soybeans adaptable even in those countries at around equator or in northern part of China.
B. Also a great deal of variation was observed out of 5 days grown soybean sprouts in asparagine content, total length, the length and thickness of hypocotyl, weight, and yield rate, etc. These facts infer that those indigenous lines can be a good source of germplasm for the development of new soybean sprout varieties.
C. Some of the lines showed as high as 8.8 yield rate of soybean sprouts, which can be grown without any chemical growth regulators. At the same time, some of the lines showed as high as 1.5 times of asparagine content compared with Pungsannamulkong which is the most widely being cultivated as soy-sprout variety in Korea at present.
D. Based on the agronomic and sprout characteristics, 19 promising lines were selected. Some of the selected lines showed nearly same seed yields as Pungsn -namulkong but those selected lines were much poorer than Pungsannamulkong in the resistance to virus and lodging, thus considered difficult to be recommended to the farmers. But those can be used as good source materials for the development of new good variety for soybean sprouts.
E. In a word, those pure inbred lines developed from this research can not directly be recommended to the farmers for cultivation but have so much valuable characteristics which are properly maintained and studied in more detail to be a precious and unique world heritage.
F. As most of soybean sprouts grown in the factories for selling at market, soybean sprouts grown up to lateral root germination, the content of asparagine in the roots are comparatively low both in the rate of asparagine out of all sprouts and in absolute amount.
G. To extract the greatest amount of asparagine from soy-sprouts, it is needed to grow sprouts more than 16 days. When sprouts were grown with ozonic water, it was possible to grow sprouts more than 16 days without any rotting problems. The content of asparagine in the sprouts were proportionally increased up to 16 days.
H. When sprouts were grown in 0.2% of urea, the content of asparagine in sprouts were significantly increased. Soybean sprouts grown at 20℃ room
temperature showed the highest yield rate and asparagine content, at the same time.
I. A serial experiments conducted to develop an antidote of alcohols revealed that "SV antidote for a hangover" made of mixture of asparagine and several kinds of soybean extracts showed better detoxifying effects than pure asparagine. A few more experiments are to be needed to commercialize the one as the selling products.
2. Development of nature plant growth conditioner (NPGC) for soybeansprouts cultivation
A. Effect of NPGC(Nature plant growth conditioner) on growth and quality of soybean sprouts
Qualities of soybean sprouts cultivated by various nature plant extracts treatment during cultivation for 6 days were investigated. Nature plant growth conditioners (NPGC) were extracted from Japanese Elm, bamboo - shoot, popolar glandnlosa, mustard by water, ethanal, ultrasonic waves and the concentrations of 0.05, 0.1 and 0.5% were adjusted. Total weight yield of WEJ (0.5% water extracts of Japanese Elm), WEM (0.5% water extracts of mustard), UEB (0.5% ultrasonic waves extract of bamboo-shoot) soybean sprouts as compared with CON (control) products increased by 9.8~11.0%.
No great difference occurred among all samples in the changes of moisture content, bacterial counts (except for WEH sample). Vitamin C contents at 6th days of cultivation were 16.1mg% for WEM soybean sprouts, 15.2mg% for UEB products 14.6mg% for WEJ 14.1mg% for INB (sample treated indolbi) and 14.3mg% for CON respectively. The orders of dietary fiber and total amino acid content were WEM > UEB > WEJ > INB > CON. and WEM > CON > WEJ > UEB > INB respectively. The levels of Fe, Mg and P in UEB soybean sprouts as compared with CON increased by 97.3, 35.8 and 22.8 respectively.
B. NPGC action mechanism to soybean sprouts by storage
Qualities of soybean sprouts cultivated by NPGC (WEJ, WEM, UEB, INB and CON) and stored for 6 days at 7℃ were investigated by moisture content, number of total microbe, vitamin C content, chlorophyll content, hardness and sensory evaluation. Moisture content of soybean sprouts stored at 7℃ in all samples increased by 2~5%. Number of total microbe also increased significantly during storage at 7℃. However, no great difference occurred among samples in the moisture content, number of total microbe, vitamin C and chlorophyll content. The orders hardness in soybean sprouts at 5 days were WEM > UEB > INB > WEJ = CON. Color score of WEJ and WEM was higher than those of the others However, higher scores for overall taste were found for WEJ products as compared to the other samples.
C. Optimal conditions and quality of soybean sprouts cultivated by WEM treatment with central composite design
To enhance soybean sprouts growth, the optimal conditions for WEM(water extracts of mustard) treatment of soybean during soaking at 20℃ were evaluated with WEM concentration (0.05, 0.1 and 0.5%), treatment time(30, 60 and 90min) and treatment freqnency (1,2 and 3 time) by response surface methodology. Optimal conditions of soybean sprouts cultivated by WEM (water extracts of mustard) during soaking of soybean were WEM concentration of 0,30 to 0.40, WEM treatment time of 34 to 38 min, and WEM frequency of ons time. The soybean sprouts cultivated under the optimal conditions increased above 12.5% germination rates.
D. Effect of optimal cultivation conditions on quality of soybean sprouts
Qualities of soybean sprouts cultivated by WEM (water extracts of mustard) treatment during cultivation for 6 days were investigated. Vitamin C content and dietary fiber content of WEM soybean sprouts as compared with CON products increased by 16.8 and 11.4%, respectively. Total amino acid yield of WEM sample as compared with CON increased slightly. The levels of P and Na in WEM soybean. sprouts increased by 8.5 and 4.5%, respectively. Number of total microbe of CON and WEM soybean sprouts during cultivation at 6 days increased 8.5 cfu/g and 7.4 cfu/g, respectively.
E. Effect of optimal cultivation conditions on quality of soybean sprouts during storage
Qualities of soybean sprouts cultivated by water extracts of mustard(WEM), and stored for 6 days at 7℃, were investigated by moisture content, number of total microbe, vitamin C content, chlorophyll content and sensory evaluation. No great difference occurred among samples in moisture content, number of total microbe, vitamin C content and chlorophyll content. However, higher scores for overall taste were found for WEM products as compared to CON sample.
F. Mass production system for nature plant growth conditioner(NPGC)
In this study, the mass production of mustard extracts powder by freeze drying for soybean sprouts cultivation was investigated. The safety of nature plant growth conditioner extracted by mustard was higher than that of the other growth conditioners(Indolbi, BA, etc), but the price was more expensive than that of the others. On the other hand, The patent (Invention title ; cultivation method of soybean and mung bean sprouts by using mustard extracts , Application number ; 10-2005-0095083) for intellectual protection was applied.
3. Improvement of the economical extraction method of asparagine and isoflavone
According to extraction condition from beaker to the mass production, asparagine and isoflavone are effectively extracted from soy and soybean sprouts cultivated during 10 days. Acetone is a excellent solvent for dissolving isoflavone from the fat-removed soy powders and the head of soybean sprouts. The removing foam process in the extraction of asparagine is a key process for easy producing and we are effectively extracted by using alcohol type surfactant (DS-1000). Specially, isoflavone and asparagine are extracted by using the head and the body from the soybean sprouts respectively. As this method utilize the industrial by-product which is obtained the process of making a head cutting soybean sprouts, we will anticipate to reduce the production cost economically.
We also established the obtaining condition for genistin and daidzin from the extracted crude isoflavone material using the solubility of genistin and daidzin in the special solvent system. It is more economical and effective method than the general column chromatographic separation method. Therefore, genistein and daidzein are directly obtained through the acid hydrolysis of glycoside genistin and daidzin. This aglucone isoflavones are very important materials for making alcohol related symptom, HRT agent (cream and gel types), and anti-cancer agent and so on.

목차 Contents

• 제 출 문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 11
• CONTENTS ... 16
• 목 차 ... 17
• 제 1 장 연구개발과제의 개요 ... 18
• 제 1 절 연구개발의 목적 ... 18
• 제 2 절 연구개발의 필요성 ... 19
• 제 3 절 연구개발의 범위 ... 24
• 제 2 장 국내외 기술개발 현황 ... 25
• 제 1 절 국내.외의 기술현황 ... 25
• 제 2 절 연구결과의 영향 ... 28
• 제 3 장 연구개발수행 내용 및 결과 ... 31
• 제 1 절 : 고기능성 나물콩품종개발과 상품화 ... 31
• 제 2 절 : 제2세부과제 : 콩나물재배용 천연 생장조정물제 개발 ... 58
• 제 3 절 : Asparagine과 isoflavone의 경제적 추출방법 개발 ... 91
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 106
• 제 5 장 연구개발결과의 활용계획 ... 109
• 제1세부과제 결과 ... 109
• 제2세부과제(협동과제) 결과 ... 109
• 3. 제3세부과제(위탁과제) 결과 ... 110
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 112
• 제 7 장 참고문헌 ... 115
• 끝페이지 ... 118