초록

○ 연구결과
쪽파 순원기 배양을 통한 기내 자구 생산을 위하여 기내인경 유도조건 및 대량생산을 위한 체세포 배 캘러스로부터의 재분화조건 등을 확립하였으며, 오염율 감소 방법 및 공기분배장치 등의 개발을 통하여 저렴하고 효율적인 대량증식 방법을 개발하였고, 또한 우량 종구 포장 생산기술을 위하여 기내 자구의 순화 및 비대를 위한 온실 및 포장재배 기술을 체계화 함.

Abstract ▼

IV. Results and Possible Application of the Study
1. Results
A. Development of production technique for in vitro bulblet by using shoot tip culture.
1) Induce of multiple shoots and disease-free stocks
Multiple shoots induction was increased when grown in MS medium supplemented 2-ip conc

IV. Results and Possible Application of the Study
1. Results
A. Development of production technique for in vitro bulblet by using shoot tip culture.
1) Induce of multiple shoots and disease-free stocks
Multiple shoots induction was increased when grown in MS medium supplemented 2-ip concentration was increased. Shoot vitrification was suppressed by osmotic regulator as sorbitol, PEG(MW;8000), NaCl. Number of shoots were increased by PEG concentration was increased. Virus-free stock production was investigated when grown in MS medium supplement with 10mg/L ribavirin.
2) Mass production of multiple shoots and bulblet growth in vitro
Multiple shoot primordium formation were increased when grown in MS medium supplemented 3mg/L 2-ip, 0.2M sorbitol, and 3% PEG(MW:8000).
Bulblet formation from scale segments culture was more at the culture in MS medium supplemented with 9% sucrose, 0.9% gelite under 24h light illumination.
Bulblet diameter was highest at cultured on MS medium supplemented with 100mg/L C.C.C.
3) Mass proliferation of bulb
Highest rates of somatic embryogenesis resulted from apical meristem cultured on MS medium supplemented with 0.5-1.0mg/L 2,4-D or picloram. Friable embryogenic callus were induced from apical meristem cultured on MS medium supplemented with picloram. Plantlet regeneration was obtained when friable embryogenic callus line #5 was transferred to MS medium supplemented with 1.0mg/L BA.
B. Development of bioreactor system for mass production of wakegi plantlets
1) Development of the bioreactor with the economical efficiency
a) Development of 15 L liquid bioreactor
- Development of the bioreactor attached an vent and sparger system prepared from the water bottle with a wide mouth
- Developement of various spargers for the efficient air injection.
b) Investigation of the reducing method of the culture contamination(air, filtration)
- Development of an apparatus of air cleanup and sparger attached membrane used for reducing contamination of bioreactor
c) Development of the air apparatus for air injection into the bioreactor from the culture system
- Develpoment of an cheap and efficient appararus of air distribution to the culture set
d) Development of the air apparatus for air injection into the bioreactor from the compressor system
e) Development of the air apparatus for mass culture of wakegi plantlet
2) Development of the effective method for mass culture of the wakegi plantlet in the 15 L bioreactor
a) Development of the mass cultue method of wakegi plantlets
- Establishment of liquid culture in 15 L bioreactor of wakegi primodia
b) Plantlet formation from the wakegi primordium cultured in the 15 L bioreactor
- Observation of vigorous plantlet formation on agar medium of the wakegi primodia cultured in 15 L bioreactor
c) Mass culture of the wakegi primordium under the light irradiation
- Investigation of the light irradiation effect on the wakegi growth
d) Effect on the primordium growth of phytohormone
- Investigation of the phytohormone effect on the wakegi growth
e) Effect on the primordium growth of the air injection strength
f) Chemical treatment for antivitrification
C. Field production technique of high quality seed bulb by using in vitro bulblet
Wakegi was grown at plastic house to determine the effect of acclimatization of in vitro bulblet, technique systematization of greenhouse and field culture for bulb enlargement and performance test of virus-free seed bulb on the field production technique of high quality seed bulb by using in vitro bulblet. The grow and development of in vitro bulblet was very healthy at 25 ℃, 60 % shade, and bottom watering in plastic tunnel culture. Whole plant is well on acclimitization by using plug tray. There was no difference according to bed soil. Over 17.5 cm × 15 cm planting density and over 5 months of culture was good on seed bulb production by using hydrophonic culture. There was no difference on emergence rate of shoots according to storage condition in short storage of seed bulb.
2. Possible Application of the Study
1) Pratical application of the developed 15 L bioreactor
- We will apply to this 15 L biorector for culture of other plants.
2) Production and supply of the virus free wakegi
- We will produce the virus free wakegi plantlet and supply to farmer using the developed 15 L bioreactor and continuously improve the bioreactor system

목차 Contents

• 제출문 ... 1
• 요약문 ... 2
• SUMMARY ... 7
• CONTENTS ... 13
• 목차 ... 14
• 제1장 연구개발과제의 개요 ... 15
• 제1절 연구개발의 목적 ... 15
• 제2절 연구개발의 필요성 ... 15
• 제3절 연구개발의 범위 ... 17
• 제2장 국내외 기술개발 현황 ... 20
• 제1절 국내기술의 현황 ... 20
• 제2절 외국기술의 현황 ... 21
• 제3절 앞으로 전망 ... 21
• 제4절 기술도입의 타당성 ... 21
• 제3장 연구개발수행 내용 및 결과 ... 23
• 제1절 순원기 배양을 통한 기내 자구 급속 증식 기술 개발 ... 23
• 제2절 생물반응기에 의한 쪽파의 대량배양 시스템 개발 ... 44
• 제3절 기내 자구를 이용한 우량종구 포장 생산기술 ... 58
• 제4장 목표달성도 및 관련분야에의 기여도 ... 69
• 제1절 연구개발 목표의 달성도 ... 69
• 제2절 관련분야에 기여도 ... 70
• 제5장 연구개발결과의 활용계획 ... 72
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 73
• 제7장 참고문헌 ... 74
• 끝페이지 ... 79