초록 ▼

○ 연구결과
ㆍ 토양으로부터 신호전달저해(quorum sensing inhibition) 활성 우수균주인 AR54, AN090571, AN090072 균주를 선발 특허 출원 및 등록 함
ㆍ 배양배지의 탄소원, 질소원 선발 및 혼합 비율 최적화에 의한 선발균주의 QSI 활성을 최대화 함
ㆍ AN090571 균주로부터 QSI 활성물질 pieridicin을 분리 정제하여 구조 분석함
ㆍ 신호전달 제어 작용기작 연구로 AN090072의 배추무름병균 병원성 관련 효소 저해활성 및 AHL 분해능을 확인하였고 AHL 분해

○ 연구결과
ㆍ 토양으로부터 신호전달저해(quorum sensing inhibition) 활성 우수균주인 AR54, AN090571, AN090072 균주를 선발 특허 출원 및 등록 함
ㆍ 배양배지의 탄소원, 질소원 선발 및 혼합 비율 최적화에 의한 선발균주의 QSI 활성을 최대화 함
ㆍ AN090571 균주로부터 QSI 활성물질 pieridicin을 분리 정제하여 구조 분석함
ㆍ 신호전달 제어 작용기작 연구로 AN090072의 배추무름병균 병원성 관련 효소 저해활성 및 AHL 분해능을 확인하였고 AHL 분해 관련 유전자 탐색 및 분해 효소 정제 함.
ㆍ 선발 균주 AN090571, AN090072의 제제화 및 제제의 독성· 안전성 시험결과 안전성을 확인
ㆍ 선발 균주의 배추 절편시험에서 배추무름병균 발병 억제능을 확인함

Abstract ▼

Traditional biological control of plant pathology is based on compounds that kill or inhibit growth of plant pathogens, but, this ways occur resistance pathogens. Therefore, this problem requires another approach and we focus on Quorum Sensing (QS). QS is community genetic regulation mechanism that

Traditional biological control of plant pathology is based on compounds that kill or inhibit growth of plant pathogens, but, this ways occur resistance pathogens. Therefore, this problem requires another approach and we focus on Quorum Sensing (QS). QS is community genetic regulation mechanism that controls microbiologica functions of medical, agricultural and industrial importance. Many of the virulenc factors needed to make this transition are regulated by cell-to-cell communication. Erwinia carotovora subsp. carotovora is agram-negative bacteria phytopathogen that causes soft-rot diseases in various commercially important plants, including Chinese cabbage. Ingram-negative bacteria, which are responsible for most plant diseases, the most common QS signals are N-acyl homoserine lactones (AHLs).
To screen novel quorum sensing inhibitor(QSI) from actinomyces, we used Chromobacterium violaceum CV026 as a test organism and we selected an actinomyces designated AR54, AN090571 and AN090072. A comparative 16S rRNA gene sequence analysis revealed that three strains are most closely related to members of the genus Kitasatospora and Streptomyces. The isolate was characterized morphologically and physiologically by the given methods in the international Streptomyces project. We investigated media optimization and culture condition of the three strains for the high activity of QSI.
Kitasatospora sp. AR54 and Streptomyces sp. AN090571, for the isolation and purification of the active compound, it was extracted by ethyl acetate and purified by silica gel, sephadex LH-20 open column chromatography, thin layer chromathography and HPLC.
Streptomyces sp. AN090072 was increased QS inhibitor activities were investigated against Chinese cabbage soft rot disease by treatment of Streptomyces sp. AN090072 in vivo. As a result, QS inhibitor produced by AN090072 to inhibit pathogenic of E.carotovora. In this study evidenced the capacity of AN090072 and its supernatant and/or enzyme as quorum sensing inhibition activity in E.carotovora, and we will study how to promising potential to use quorum sensing signals as molecular target for disease control, thereby broadening current approach for prevent of plant bacterial infection.