초록 ▼

○ 연구결과
4CL 유전자와 SHT 유전자를 대장균에 동시발현 시킴으로서, 세로토닌유도체를 대장균에서 대량 생산할 수 있는 자료를 제공하였다. 또한 P450 효소인 T5H 유전자와 TDC 유전자를 동시발현 시킴으로서, 대장균에서 세로토닌을 생산할 수 있는 발현 시스템을 개발하였다. 세로토닌유도체는 radical을 제거하는 항산화 활성이 탁월하였으며, 피부세포와 간세포를 이용한 세포 실험에서도 높은 H2AX의 인산화를 가장 강력하게 억제하였으며, serotonin이 그 뒤를 이었으나, CS, FS는 이러한 활성을 보이지 않았다.

○ 연구결과
4CL 유전자와 SHT 유전자를 대장균에 동시발현 시킴으로서, 세로토닌유도체를 대장균에서 대량 생산할 수 있는 자료를 제공하였다. 또한 P450 효소인 T5H 유전자와 TDC 유전자를 동시발현 시킴으로서, 대장균에서 세로토닌을 생산할 수 있는 발현 시스템을 개발하였다. 세로토닌유도체는 radical을 제거하는 항산화 활성이 탁월하였으며, 피부세포와 간세포를 이용한 세포 실험에서도 높은 H2AX의 인산화를 가장 강력하게 억제하였으며, serotonin이 그 뒤를 이었으나, CS, FS는 이러한 활성을 보이지 않았다. 또한 산화적 스트레스에 의해 단백질의 carbonylation 또한 증가하는데 0.5 mM H₂O₂를 처리한 HaCaT 세포의 단백의 carbonyl content가 증가함을 관찰하였다. 산화적 스트레스에 의한 carbonylation을 억제하는 활성은 SR가 가장 높았으며, FS, CaS가 그 뒤를 이었다. 또한 세로토닌유도체를 기능성화장품 소재로 활용하여 기능성화장품 시제품을 제작하였고, 추후 상업화를 시도하기 위해 기술이전을 실시하였다.

Abstract ▼

Results and Suggestion on Utilization of Results:
Plant-specific phenylpropanoid amides were produced in a recombinant Escherichiacoli that expressed from Arabidopsis and from pepper plants. Upon exogenous treatment with several precursors, high concentrations of the following phenylpropanoid ami

Results and Suggestion on Utilization of Results:
Plant-specific phenylpropanoid amides were produced in a recombinant Escherichiacoli that expressed from Arabidopsis and from pepper plants. Upon exogenous treatment with several precursors, high concentrations of the following phenylpropanoid amides were produced abundantly in the culture medium in a few hours: 4-coumaroylserotonin (215 mg/l), 4-coumaroyloctopamine (208 mg/l), and 4-coumaroyltyramine (187 mg/l). Furthermore, we employed two different sources of 4CL cDNAs which are of Arabidopsis 4CL1 and rice 4CL1 in order to examine the yield of serotonin derivatives in E. coli. In addition, we investigate the cytoprotective effects of serotonin derivatives based on intracellular reactive oxygen radical (ROS) generation, lipid peroxidation, protein carbonylation, and phosphorylation of histone H2AX in H₂O₂ treated-HepG2 and HaCaT cells. We have shown that HCAAS showed various strong antioxidant activities in hydrogen peroxide treated both cell lines, suggesting that these compounds may play as chemotherapeutic agents for preventing or reducing the oxidative stress-induced diseases. In this project, we evaluated the serotonin derivatives's efficacy on skin cells. Serotonin derivatives showed whitening effect, anti-inflammatory effect, anti-fibrosis effect and anti-lipogenesis effect. It also investigated its physicochemical characteristics and performed formulation research for ingredients using liposomal formulation to maximize its stability and transdermal delivery. When stability of liposomal formulation of serotonin derivatives was examined under various circumstances (temperature, light, ), it was found to be suitable to be used as a raw material of cosmetics. Toxicity test with human models to assess its safety also revealed that serotonin derivatives were safe materials. In addition, serotonin derivatives were registered in ingredient list of International Cosmetic Ingredient Dictionary and Handbook's(CTFA). Outcomes from this project are expected to be commercialized in functional cosmetic's area.