보고서 정보
주관연구기관 |
고려대학교 Korea University |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2011-04 |
과제시작연도 |
2010 |
주관부처 |
농림축산식품부 Ministry of Agriculture, Food and Rural Affairs(MAFRA) |
등록번호 |
TRKO201400026397 |
과제고유번호 |
1545001804 |
사업명 |
고부가가치식품기술개발['10신규] |
DB 구축일자 |
2014-11-10
|
DOI |
https://doi.org/10.23000/TRKO201400026397 |
초록
○ 연구결과
첫 번째는 한국산 잣에 함유되어 있는 Δ-5계열 기능성 지방산인 피놀레닌산 (pinolenic acid)을 효소를 이용한 에타놀리시스 반응을 통하여 최대 37%부터 70% 수율을 갖는 25% 이상의 피놀레닌산 농축물을 얻을 수 있었다. 두 번째는 효소를 이용하여 피놀레닌산 함유 모노글리세라이드를 합성할 수 있는 최적화 조건을 확립하였을 뿐만 아니라 제품에 적용하는 실험까지 성공적으로 완료하였다.
Abstract
▼
IV. Results
Pinolenic acid (PLA)was enriched vialipase-catalyzed ethanolysis from pine nut oil in a batch reaction system and a continuous reactio nsystem (packedbedreactor:PBR) using a commercial immobilized lipase. Fatty acid composition of five edible nuts such as pine nut, walnut,casunut,almo
IV. Results
Pinolenic acid (PLA)was enriched vialipase-catalyzed ethanolysis from pine nut oil in a batch reaction system and a continuous reactio nsystem (packedbedreactor:PBR) using a commercial immobilized lipase. Fatty acid composition of five edible nuts such as pine nut, walnut,casunut,almond,and pistachio was investigated. PLA in all nut sexcept pine nut was not detected.Three immobilized lipases were screened for their abilities to enrich pinolenic acid by ethanolysis. Novozym 435 from Candida antartica was selected for enrichment of pinolenic acid from pine nut oil. Effect of several parameters such as molar ratio (pine nut oil to ethanol),temperature, enzyme loading, and moisture content in a batch reaction system was investigated. Optimal conditions for enrichment of pinolenic acid in a batch reaction system were molar ratio of 1:80 (pine nut oil:ethanol), temperature of 35℃, enzyme loading of10 wt% (wt% on substrate weight),and moisture content of 250 ppm, respectively. The same type of experiments in a recyc ling packed bed reactor(PBR) as a continuous reaction system was investigated. Effect of several parameters such as molar ratio (pine nut oil to ethanol),temperature,and residence time in reactor was investigated. Optimal conditions for enrichment of pinolenic acid in PBR system were molar ratio of 1:75 (pine nut oil:ethanol), temperature of 45℃,and 3 min of residence time, respectively. Consequently, pinolenic acid was effectively enriched from pine nut oil by lipase-catalyzed ethanolysis. Moreover, PBR system was more effective than a batch reaction system when recovery yield, saving energy, and industrial aspect were considered.
High purity monoglyceride was produced in a solvent free system from pine nut oil by enzymatic reaction. To synthesize monoglyceride, two different enzymatic reactions namely glycerolysis, and esterification were tested and esterification was more suitable reaction than glycerolysis. Two immobilized lipases and two free type lipase were screened for their ability to produce monoglyceride using glycerol and fatty acids (derived from pine nut oil) as substrates. Lipase G from Penicillium camembertii, which is a nonspecific lipase, gave the highest activity. Hence, in subsequent trials, Lipase G was employed to catalyze the production of monoglyceride containing PLA residues. Effect of several parameters such as temperature, molar ratio (fatty acid to glycerol),moisture, and enzyme loading was investigated. Optimal conditions for production of monoglyceride in batch reaction system were molar ratio of 1:5 (fatty acid to glycerol),temperature of 20℃,and enzyme loading of 600 U/g (by weight of total substrates),an d moisture content of 2%,respectively. Vacuum was applied to control moisture content in reaction mixture. Trials for starting time of vacuum was carried out and optimal condition was after reaction time of 4 h. Effect of degree of vacuum on the esterification between fatty acid and glycerol for production of monoglyceride was investigated. Optimal degree of vacuum was 1 torr. Incubation trials for production of high purity monoglyceride were carried out at low temperatures. The temperatur eranges tested was between 10 and -20℃. Optimal incubation conditions were incubation temperature of -10℃, and incubation time of 6 days.Consequently, high purity monoglyceride from pine nut oil was successfully produced by esterification of fatty acids derived from pine nut oil with glycerol using a free type lipase from Penicillium camembertii (Lipase G). The concentration and purity of monoglyceride produced at optimal condition was 88%, and 91%, respectively. In particular, distillation step can be omitted by utilization of technology developed in this study.
The monoglyceride containing PLA was applied to produce two processed foods namely mayonnaise, and soy milk. There was no difference in the emulsion stability between commercial monoglyceride (containing stearic acid) and monoglyceride containing PLA produced from this study. Moreover, synergistic effect in the emulsion stability of mayonnaise was showed when mixed monoglyceride of commercial monoglyceride, and monoglyceride containing PLA was used. There was no significant difference in the sensory evaluation between two soy milks prepared with commercialmonoglyceride,and monoglyceride containing PLA. Consequently, monoglyceride containing PLA produced from this study was useful emulsifier to produce emulsion foods namely mayonnaise, and soy milk.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.