초록

○ 연구결과
-죽자초추출물로부터 살충활성물질 분리동정 :알칼로이드 화합물 2종
-표준화
-죽자초 시제품 살충효과 검정 및 농가실증 :진딧물 방제가 88∼98%
-제형화
-죽자초 시제품 안전성 평가 :급성경구,급성경피,어독성,잔류농약,중금속
-시제품의 적용확대시험 :꽃매미 93.3% 살충효과

Abstract ▼

Ⅳ. Study Results
1. Isolation and identification of insecticidal components from Papaveraceae Plantsonaphids
Alcohol extracts from the seed of Macleaya cordata have shown a significant insecticidal effect against the growth of the cotton aphid, Aphis gossypii Glover. In this study, we aimed to

Ⅳ. Study Results
1. Isolation and identification of insecticidal components from Papaveraceae Plantsonaphids
Alcohol extracts from the seed of Macleaya cordata have shown a significant insecticidal effect against the growth of the cotton aphid, Aphis gossypii Glover. In this study, we aimed to isolate the principal component of M. cordata to manifest its insecticial effect on A. gossypii. The dried seed of M. cordata were extracted with 80% aqueous MeOH, and the concentrated extracts were partitioned successively with EtOAc, n-BuOH, and H₂O. During a search for an insecticidal compound against the aphids, activity-guided fractionation led to the isolation of two alkaloids from an n-BuOH fraction through repeated silica gelcolumn chromatographic separations. Based on NMR, MS, and IR spectroscopic data, the chemical structures of the compounds were determined to be 8-hydroxydihydrochelerythrire and 8-methoxydihydrosanguinarine, respectively. This was the first study in which 8-methoxydihydrosanguinarine was isolated from M. cordata.
8-Hydroxydihydrochelerythrine and 8-methoxydihydrosanguinarine showed significant insecticidal effects against the cotton aphid,73.6±14.6% and 76.1±7.9% at100ppm, respectively
Tubers of Corydalis turtschaninovii were extracted with 80% aqueous MeOH, and the concentrated extract was successively partitioned with n-hexane, EtOAc, n-BuOH, and H₂O.An activity-guided fractionation to search forinsecticidalcompounds againstaphids led to the isolation of six alkaloidsfrom the n-hexane and EtOAcfractionsusing arepeatedsilicagel, an octadecylsilicagel(ODS), and Sephadex LH-20column chromatographic separations. Based on the spectroscopic data of NMR, MS, and IR, the chemicalstructures of the compounds were determined to be:(+)-stylopine (1), (+)-corydaline (2), demethylcorydalmine (3), isocorypalmine (4), glaucine (5), and pseudoprotopine (6). The compounds showed significantinsecticidal effects against A. gossypii, 69.7±Q7.2%, 46.9±m2.4%, 68.5±8.6%, 75.5±･4.2%, 80.2±9.7%,and78.9±11.3% at 1000ppm, respectively.
2.Quantitative analysis of index components using HPLC
HPLC method for quantitative analysis of index components was established. Index components were designated as three insectividal alkaloids isolated from Papaveraceae Plants. HPLC analysis was carried out for quantification of index components in the sample products.
To test the stability of the insecticidal components in the sample products HPLC anlysis was performed and index component was used by sanlguinarine. The sample products were sprayed on the leaves of tomato and the leaves were collected on 1, 3, 7, and 14 days after. The collected leaves were freeze-fryed and used for HPLC analysis. The index component, sanguinarine, was detected in the leaves collected only on 1 day after.
3. Manufacture of sample products
To select the best solvents to manufacuture sample products, five solvents including methanol and ethanol was used to extract Macleaya cordata.
Among them, ethanol extracts showed the highest insecticial activity.
20 sample products were manufactured using Macleaya cordata extracts, Chajhu Extract, tobacco extracts and some minerals and evaluated for emulsibility, throwing power, and insecticidal activity on aphids. To investigate the insecticidal characteristics of sample products, the variance of insecticidal effect according to the treatment concentration and life clycles of aphids, expression rate of drug effects, toxicity, residue of drug effect, penetration and translocation of drugs were evaluated. The sample products including 1,000 ppm active materials showed significant insecticidal activity through all life cycles of aphids, and insecticidal effects was expressed from 24 hrs after treatment of drugs.
4. Evaluation for insecticidal effects of sample products
Macleaya cordata extracts were treated on the leaves of cucumber and red pexpper cultivated in pots, and it showed very high insecticidal effects against the cotton aphid on cucumber and red pepper 98.7 and 96.0, respectively. The same test performed for cucmber cultivated in greenhouse also showed the significant insecticidal effexts against the cotton aphid 87.6% and 98.0% at 3 days and 7 days, respectively. after treatment.
Macleaya cordata extracts also showed significant insecticidal effects against different insects, Limois emelianocvi Oshanin and leafhopper, which was almost same activiy as the well-known insecticides, derris, sophora and a pyrethrum flower extracts.
5. Safety test of sample products
Safety test of sample products was carried out as for oral tixicity, dermal toxicith, and fish toxicity to submit to public-announcenent list as environment-friendly organic agricultural materials. Fish toxicity was evaluated as III grade, LD50 values in acute oral toxicity and dermal toxicity were determined as 1111.5 and 2000 mg/kg, respectively, which indicated the sample product was very safe comparing to registration standard value, 200mg/kg. And analysis of 177 agricultural pesticides and 8 heavy metals including Zn and Cu in the sample products showed no detection in the samples.
6. Industralization of sample products
To steady secure raw materials, Macleaya cordata, cultivation methods in domestic and foreign area were studied. Finally, Supply contract was agreed with a manufacture company at Hoonan province in Chian, which is the major production area of Macleaya cordata. And mass production of the extracts using 1500 L extractor was establshed. The sample materials was public-announced as the list of environment-friendly organic agricultural materials. No. of environment-friendly organic agricultural materials, Choongstar, was 11-유기-5-307, which will be available for two years and public-announced on the home-page of RDA until A pril 13th in 2013.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 10
• CONTENTS ... 17
• 목차 ... 18
• 제1장 연구개발과제의 개요 ... 19
• 제1절 연구개발의 목적 및 필요성 ... 19
• 제2절 연구개발의 범위 및 연구내용 ... 21
• 제2장 국내외 기술개발 현황 ... 23
• 제1절 생산 및 시장현황 ... 23
• 제2절 국내외 기술개발 현황 ... 25
• 제3장 연구개발수행 내용 및 결과 ... 26
• 제1절 식물 유래 진딧물 살충 물질의 분리 동정 ... 26
• 1. 진딧물 살충용 식물추출물 선발 ... 26
• 2. 현호색으로부터 진딧물 살충 물질의 규명 ... 35
• 제2절 HPLC를 이용한 지표 성분 분석 방법 확립 및 표준화 ... 42
• 1. HPLC를 이용한 지표성분의 분석 ... 42
• 2. HPLC를 이용한 제품의 안정성 분석 ... 50
• 제3절 제형화를 통한 시제품 개발 ... 54
• 1. 제형화를 위한 최적용매 선발 ... 54
• 2. 시제품제조 ... 56
• 3. 시제품의 살충작용 특성 조사 ... 58
• 제4절 시제품의 해충 살충효과 검정 ... 62
• 1. 시제품의 진딧물 살충효과 검정 ... 62
• 2. 시제품의 진딧물 살충효과 농가실증 ... 66
• 3. 시제품의 적용확대시험 ... 68
• 제5절 시제품의 안전성 평가 ... 71
• 1. 시제품의 독성 평가 ... 71
• 2. 시제품의 환경안전성 평가 ... 74
• 제6절 시제품의 산업화 연구 ... 77
• 제4장 목표달성도 및 관련분야에의 기여도 ... 85
• 제1절 목표달성도 ... 85
• 제2절 기술발전 기여도 ... 88
• 제5장 연구개발 성과 및 성과활용 계획 ... 89
• 제1절 연구개발성과 ... 89
• 제2절 성과활용계획 ... 98
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 100
• 제7장 참고문헌 ... 101
• 끝페이지 ... 103