초록 ▼

ㅁ 본연구는 신경세포 특이적인 패턴인식수용체의 발현과 그 기능을 밝히는 연구임. 본 연구를 통하여 다음과 같은 연구결과를 얻었음.
√ 뇌피질, 해마신경세포, 신경세포주 배양 후, 패턴인식수용체 발현을 테스트함. 그 결과 TLR2, 9, 11, 13 mRNA및 TLR12의 단백질 발현을 확인함. 또한 해마 신경세포주 HT22에서 TLR1, 2, 3, 4, 6, 8, 9, 12의 발현을 확인 함.
√ 일차배양 DRG 신경세포에 발현되는, TLR3, 4, 7의 agonist 인, polyIC, LPS, R837은 신경세포 사멸

ㅁ 본연구는 신경세포 특이적인 패턴인식수용체의 발현과 그 기능을 밝히는 연구임. 본 연구를 통하여 다음과 같은 연구결과를 얻었음.
√ 뇌피질, 해마신경세포, 신경세포주 배양 후, 패턴인식수용체 발현을 테스트함. 그 결과 TLR2, 9, 11, 13 mRNA및 TLR12의 단백질 발현을 확인함. 또한 해마 신경세포주 HT22에서 TLR1, 2, 3, 4, 6, 8, 9, 12의 발현을 확인 함.
√ 일차배양 DRG 신경세포에 발현되는, TLR3, 4, 7의 agonist 인, polyIC, LPS, R837은 신경세포 사멸에 영향이 없음을 확인 함.
√ 대신 R837이 특정한 감각신경세포 내 칼슘 신호를 유발한다는 새로운 사실을 발견함.
√ 또한 R837에 의해 극심한 itch behavior가 유발됨을 행동학적 실험으로 발견함.
√ TLR3 agonist인 poly(IC)에 의해 DRG 감각신경세포 사멸이 유도됨.
√ DRG 일차배양 감각세포에서 imiquimod 처리 후, 일군의 세포 내 칼슘 신호가 증가하는 것을 확인 함.

Abstract ▼

Recent studies show that pattern-recognition receptors, originally identified on the innate immune cells, are also expressed in certain neuronal cell types. Furthermore, it was suggested that these pattern-recognition receptors are involved in the neuronal cell death, and proliferation and different

Recent studies show that pattern-recognition receptors, originally identified on the innate immune cells, are also expressed in certain neuronal cell types. Furthermore, it was suggested that these pattern-recognition receptors are involved in the neuronal cell death, and proliferation and differentiation of neuronal stem cells. In this regard, we propose to systemically characterize the expression profiles of all members of pattern-recognition receptors in different subtypes of neurons. Then, we will elucidate the role of pattern-recognition receptors in neuronal cell death, and differentiation and proliferation of neuronal stem cells.
For this purpose, we will perform the following investigations.
 Systemic characterization of pattern-recognition receptors in different subtypes of neurons
-- characterize the constitutive expression profile of pattern-recognition receptors
-- characterize the regulation of pattern-recognition receptors after various death-inducing stimuli
 Regulation of neuronal cell death via pattern-recognition receptors
-- characterize the neuronal cell death after pattern-recognition receptor stimuli
-- mecahnism of neuronal cell death after pattern-recognition receptor stimuli
 Study on the proliferation and differentiation of neurons via pattern-recognition receptors
-- Effects of pattern-recognition receptors on embyonic neuronal stem cell differentiation
-- Effects of pattern-recognition receptors on embryonic neuronal stem cell proliferation
 Studies on the neuron-specific intracellular signaling pathways via pattern-recognition receptors
-- intracellular signaling pathways involved in neuronal cell death
-- intracellular signaling pathways involved in neuron proliferation and differentiation
 Development of strategy for neuroprotection targeting pattern-recognition receptors
-- neuroprotective molecule screening using organotypic hippocampal slice culture system
-- Target/drug validation in in vivo neurodegeration animal model